Free Powerpoint Templates Page 1 Free Powerpoint Templates Electrophoretic Mobility Shift AssayElectrophoretic Mobility Shift Assay (EMSA) Service by Profacgen.

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Free Powerpoint Templates Page 1 Free Powerpoint Templates Electrophoretic Mobility Shift AssayElectrophoretic Mobility Shift Assay (EMSA) Service by Profacgen

Free Powerpoint Templates Page 2 First Page : Electrophoretic mobility shift assay (EMSA, also called gel retardation assay or gel shift assay) is an in vitro method to detect the interaction between proteins and nucleotides. It is originally used to detect transcription factors, and is now further developed into investigating DNA-protein interactions, RNA-protein interactions, and even DNA-RNA interactions. Profacgen provides professional EMSA service to identify potential DNA or RNA binding proteins with high efficiency and accuracy.

Free Powerpoint Templates Page 3 Second Page : The principle of EMSA is based on the electrophoretic mobility discrepancy between biomolecules with different molecular masses. Nucleotides move slower during electrophoresis when bound with proteins, thereby giving a lagged band in the final detection. EMSA can be used to qualify and quantify proteins that specifically bind to given nucleotides. We can evaluate the binding affinity according to the quantity of the lagged band, and even calculate the associate and dissociate constant. EMSA is often performed concurrently with DNase I footprinting assay and primer extension experiments when studying transcription initiation, DNA replication, DNA repair or RNA processing and maturation.DNase I footprinting assay

Free Powerpoint Templates Page 4 Profacgen has a mature EMSA service experiment platform and extensive experience in nucleotide-protein binding analysis. We perform strict examination on both nucleotide and protein to ensure they are qualified for EMSA before the initiation of each project. We can use radioactive isotope and also other labeling agents such as biotin and FAM to label nucleotides. Unlabeled nucleotides are used as specific competitors to eliminate non- specific nucleotide-protein interactions. We also have advanced instrument for signal detection ensuring high sensitive and high signal-to-noise ratio results.EMSA service

Free Powerpoint Templates Page 5 Service work flow: Nucleotide and protein preparation (optional) Protein and nucleotide QC test (EMSA will be stopped if QC fails) Nucleotide labeling and purification Electrophoresis of nucleotide-protein complexes Membrane transfer and cross-link by UV (optional) Signal detection

Free Powerpoint Templates Page 6 Our advantages: State-of-the-art technological platform Short turn-around time Best price in the market Consistent results and detailed experimental reports

Free Powerpoint Templates Page 7 Contact Profacgen Mailing Address: 45-1 Ramsey Road, Shirley, NY 11967, USA Tel: Website:

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