Products > HEK-293 Transfection Reagent (Epithelial Kidney Cells) Altogen Biosystems offers the HEK-293 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. HEK-293 Transfection Reagent is an advanced formulation of lipid-based reagents, and it is developed to provide high transfection efficiency with HEK-293. This cell line is a good host for studying kidney cells. When cultured in vitro, HEK-293 cells are a monolayer and adhere to the surface of the culture flask. Other applications include drug discovery, gene expression studies, molecular and cell biology research applications. Purchase HEK-293 Transfection Kit at Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA Telephone   Fax   

HEK-293 Cell Line Description and Applications Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA Telephone   Fax    Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products High Density HEK-293 Atcc.org Products > HEK-293 Transfection Reagent (Epithelial Kidney Cells) The HEK-293 cell line originates from human embryonic kidney cells in 1977 that were transformed with sheared adenovirus 5 DNA in tissue culture. The HEK-293 cell line is described as a cellular test tube because of its application to a variety of research. The HEK- 293 transformation incorporated the viral genome into human chromosome 19. HEK-293 cells have applications for research related to gene therapy and therapeutics.

HEK-293 Transfection Protocol 1. Plate 10, ,000 HEK-293 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection 2. Wash with 1xPBS and add 0.5 ml of fresh growth medium 3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and 750 ng DNA (or mRNA), or 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for minutes 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with HEK-293 cells (from step 2) 7. Incubate cells at 37ºC in a humidified CO 2 incubator 8. Assay for phenotype or target gene expression hours after transfection Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection If the viability of HEK-293 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA Telephone   Fax    Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products Products > HEK-293 Transfection Reagent (Epithelial Kidney Cells)

General Lipoplex-mediated Transfection Mechanism of Action Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA Telephone   Fax    Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products Products > HEK-293 Transfection Reagent (Epithelial Kidney Cells)

HEK-293 Transfection Kit Product Details Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA Telephone   Fax    Two component formulation enhances lipid mediated transfection efficiency Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA Kit includes Transfection Enhancer reagent and recommended transfection protocol High transfection efficacy in the presence of serum Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid Reproducible transfection results Works well for standard reverse transfection and high-throughput applications Developed and manufactured by Altogen Biosystems ( Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products Products > HEK-293 Transfection Reagent (Epithelial Kidney Cells)

Data Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into HEK-293 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT- PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3). Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products Products > HEK-293 Transfection Reagent (Epithelial Kidney Cells)

Data Figure 2. Protein expression of Lamin A/C in HEK-293 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into HEK-293 cells following Altogen Biosystems transfection protocol. At 72 hours post- transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products Products > HEK-293 Transfection Reagent (Epithelial Kidney Cells)

Pre-optimized transfection protocol for HEK-293 cell line Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing Free of serum and protein of animal origin Compatible with standard and reverse transfection methods (both protocols provided in the kit manual) Easy to use HEK-293 transfection protocol ensures great performance with expedited experimental timeline Equally efficient for single or multiple transfections Can be used for transient transfection and development of stable HEK-293 cell lines Bio-degradable after endocytosis Used for preclinical research worldwide HEK-293 Transfection Kit Benefits Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA Telephone   Fax    Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products Products > HEK-293 Transfection Reagent (Epithelial Kidney Cells)