1. Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-N-SH Transfection Reagent (Neuroblastoma Cells, HTB-11)
Altogen Biosystems offers the SK-N-SH Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The SK-N-SH Transfection Reagent is an advanced cationic lipid formulation reagent, and it has been developed to provide high transfection efficiency with the SK-N-SH cell line.
This cell line is a good host for studying brain cancer and cell and molecular biology. When cultured in vitro, SK-N-SH cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase SK-N-SH Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. High Density SK-N-SH cells
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-N-SH Transfection Reagent (Neuroblastoma Cells, HTB-11)
SK-N-SH Cell Line Description and Applications
The SK-N-SH cell line originates from a 4-year old female patient suffering from neuroblastoma. J. L. Biedler established this cell line from the brain tissue of the patient in The SK-N-SH cell line has a high population doubling time and also produces large amounts of dopamine-beta-hydroxylase. The cell has a modal chromosome number of 47, two x chromosomes (and therefore is genetically female), and also contains an abnormality: trisomy 1q. Research indicates this cell line is adenosinergic, glutamatergic and acetylcholinergic. After amyloid-beta peptide treatment, SK-N-SH will increase production of M-CSF. This cell is a good transfection host.
High Density SK-N-SH cells
Atcc.org
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. SK-N-SH Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-N-SH Transfection Reagent (Neuroblastoma Cells, HTB-11)
SK-N-SH Transfection Protocol
1. Plate 10, ,000 SK-N-SH cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with SK-N-SH cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of SK-N-SH cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-N-SH Transfection Reagent (Neuroblastoma Cells, HTB-11)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. SK-N-SH Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-N-SH Transfection Reagent (Neuroblastoma Cells, HTB-11)
SK-N-SH Transfection Kit Product Details
A cationic lipid formulation
Transfection protocols provided for transfection of proteins, DNA, mRNA, siRNA, shRNA and microRNA
Transfection Enhancer reagent provided with the kit
Produce higher level of recombinant protein expression with minimal disruption of normal cell function
Generate physiologically relevant data you can trust
Effective for plasmid DNA/siRNA co-transfection
Easy-to-use transfection protocol with reproducible results
Low cytotoxicity
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-N-SH Transfection Reagent (Neuroblastoma Cells, HTB-11)
Data
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into SK-N-SH cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-N-SH Transfection Reagent (Neuroblastoma Cells, HTB-11)
Data
Figure 2. Protein expression of Lamin A in SK-N-SH cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into SK-N-SH cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. SK-N-SH Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-N-SH Transfection Reagent (Neuroblastoma Cells, HTB-11)
SK-N-SH Transfection Kit Benefits
Pre-optimized transfection protocol for SK-N-SH cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use SK-N-SH transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable SK-N-SH cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   