Date of download: 7/3/2016 Copyright © 2016 SPIE. All rights reserved. Static autoregulation. The classic static autoregulation curve shows a plateau of cerebral blood flow (CBF) versus mean arterial pressure (MAP) in the MAP range from about 50 mmHg to about 170 mmHg. Figure Legend: From: Cerebral blood flow and autoregulation: current measurement techniques and prospects for noninvasive optical methods Neurophoton. 2016;3(3): doi: /1.NPh

Date of download: 7/3/2016 Copyright © 2016 SPIE. All rights reserved. Dynamic autoregulation. Schematic illustration of the mean arterial pressure (MAP) and cerebral blood flow (CBF) transients induced by the fast release of pneumatic thigh cuffs (at time 0) for the assessment of dynamic autoregulation. MAP quickly drops and CBF passively follows this fast change. Then MAP remains at a value lower than at baseline for about 5 s, and during this time autoregulation mechanisms increase CBF toward its precuff-release value. After this initial ∼ 5 s period, baroreflex mechanisms increase MAP, and CBF changes reflect both MAP changes and autoregulatory effects. Figure Legend: From: Cerebral blood flow and autoregulation: current measurement techniques and prospects for noninvasive optical methods Neurophoton. 2016;3(3): doi: /1.NPh

Date of download: 7/3/2016 Copyright © 2016 SPIE. All rights reserved. Three basic approaches to the measurement of cerebral blood flow. (a) The Fick principle, (b) the central volume principle, and (c) the Doppler effect or autocorrelation methods. (a) A global CBF measurement is based on recording time traces of the arterial and venous blood concentrations ([x]a and [x]v, respectively) of a diffusible and physiologically inert intravascular tracer x over a time Δt that is sufficiently long to achieve equilibrium in the blood–brain tracer diffusion (in the case of the Kety–Schmidt method, the intravascular tracer was nitrous oxide (N2O), breathed continuously by the subject [see Sec ]). (b) A regional CBF measurement in a volume of interest VT is based on the measurement of the temporal dynamics of the arterial and tissue concentration (Ca and CT, respectively) of an intravascular bolus (H and A represent the peak value and the total area under the curve of the temporal trace of CT (this approach is the basis for a number of nuclear medicine [Sec. 3.3], X-ray [Sec. 3.4], and MRI techniques [Sec. 3.5]). (c) The Doppler effect applies to ultrasound or optical waves that interrogate the brain tissue at a certain frequency f, and results in a frequency shift (Δf) and in a decay rate for the normalized intensity autocorrelation function (g2) that are directly related to the speed of blood flow (these methods are employed by Doppler ultrasound [Sec ], laser Doppler flowmetry [Sec ], and diffuse correlation spectroscopy [Sec ]). Figure Legend: From: Cerebral blood flow and autoregulation: current measurement techniques and prospects for noninvasive optical methods Neurophoton. 2016;3(3): doi: /1.NPh

Date of download: 7/3/2016 Copyright © 2016 SPIE. All rights reserved. Arterial spin labeling (ASL). Schematic representation of the basic approach for blood flow measurement with arterial spin labeling MRI. The water in the arterial inflow to the brain is magnetically tagged in the labeling plane. In the imaging plane, the change in tissue magnetization is directly related to blood flow and yields a measure of CBF. Figure Legend: From: Cerebral blood flow and autoregulation: current measurement techniques and prospects for noninvasive optical methods Neurophoton. 2016;3(3): doi: /1.NPh

Date of download: 7/3/2016 Copyright © 2016 SPIE. All rights reserved. Transcranial Doppler ultrasound. (a) The location of the transtemporal window on the left side of a human subject. (b) A transcranial Doppler (TCD) probe placed against the left transtemporal window has access to the left MCA for measuring blood flow velocity. Reproduced from Ref. 85 with permission. Figure Legend: From: Cerebral blood flow and autoregulation: current measurement techniques and prospects for noninvasive optical methods Neurophoton. 2016;3(3): doi: /1.NPh

Date of download: 7/3/2016 Copyright © 2016 SPIE. All rights reserved. Thermal diffusion flowmetry. Schematic diagram of the thermal diffusion probe (TDP) showing the active, heated thermistor at the probe tip, which produces a thermal measurement field in the surrounding tissue. The size of the thermal measurement field is dependent on the tissue thermal properties and the perfusion: high perfusion produces a smaller thermal field. The diameter of the field (2r) is approximately 4 mm for typical values of thermal properties and perfusion. The passive thermistor, mounted 5 mm proximal to the probe tip, monitors the tissue baseline temperature variations. Reproduced from Ref. 92 with permission of Springer. Figure Legend: From: Cerebral blood flow and autoregulation: current measurement techniques and prospects for noninvasive optical methods Neurophoton. 2016;3(3): doi: /1.NPh

Date of download: 7/3/2016 Copyright © 2016 SPIE. All rights reserved. Diffuse correlation spectroscopy (DCS). (a) Schematic of typical DCS instrumentation that consists of a long-coherence length source coupled to a multimode fiber for light delivery to the tissue, photon-counting detector(s), and an auto-correlator board that computes the intensity of the autocorrelation function, g2(τ), based on photon arrival times (b), (c) Sample g2(τ) curves obtained over the frontal cortex in a subject under baseline conditions (black) and under hypercapnia (3% inspired carbon dioxide, gray). The increased decay rate of g2(τ) during hypercapnia reflects the increase in CBF by vasodilation. Reproduced from Ref. 104 with permission of the Society of Photo Optical Instrumentation Engineers (SPIE). Figure Legend: From: Cerebral blood flow and autoregulation: current measurement techniques and prospects for noninvasive optical methods Neurophoton. 2016;3(3): doi: /1.NPh

Date of download: 7/3/2016 Copyright © 2016 SPIE. All rights reserved. Finger photoplethysmography. A photoplethysmography sensor, which is an optical system featuring a light emitting diode (LED) and an optical detector (photodiode), is built within a finger inflatable cuff. The pressure in the finger cuff is adjusted by an air control unit placed around the wrist of a subject. When no pressure is applied in the cuff (left panel) the optical signal detected is sensitive to the arterial expansion and contraction at the cardiac rate and shows typical intensity fluctuations due to the systolic and diastolic phases of the heart cycle (“Pulse wave” in the figure). In other words the features of the optical signal reflect the dynamic changes in arterial blood volume. When the finger cuff is inflated by a fast pneumatic servo system to achieve arterial unloading at zero transmural pressure (i.e., the arterial pressure equals the cuff pressure) the optical signal flattens out (right panel). In this situation, there is no change in the arterial blood volume and the blood flows in and out of the arterial compartment are the same (“constant flow rate” in the figure). Reproduced from Ref. 139 with permission. Copyright © 2014 Elsevier Masson SAS. All rights reserved. Figure Legend: From: Cerebral blood flow and autoregulation: current measurement techniques and prospects for noninvasive optical methods Neurophoton. 2016;3(3): doi: /1.NPh