Date of download: 6/25/2016 Copyright © 2016 SPIE. All rights reserved. (a) Comparison of the SERS spectrum from the S440 reporter molecule (inset) and.

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Date of download: 6/25/2016 Copyright © 2016 SPIE. All rights reserved. (a) Comparison of the SERS spectrum from the S440 reporter molecule (inset) and 800-nm peak-emission quantum dots, both excited at 785 nm, and (b) schematic of SERS AuNPs with 60-nm colloidal gold core, adsorbed SERS reporter molecule, 30-nm thick silica encapsulant, and polyethylene glycol surface coating. Figure Legend: From: Filter-based method for background removal in high-sensitivity wide-field-surface- enhanced Raman scattering imaging in vivo J. Biomed. Opt. 2012;17(7): doi: /1.JBO

Date of download: 6/25/2016 Copyright © 2016 SPIE. All rights reserved. Raman spectra of 40 pM control gold nanoparticles (no reporter molecule: dotted line) and SERS-active nanoparticles (solid line) measured on a background of ex vivo mouse skin; SERS signals are typically a small perturbation ( ≪ 5%) on the broad tissue background. Figure Legend: From: Filter-based method for background removal in high-sensitivity wide-field-surface- enhanced Raman scattering imaging in vivo J. Biomed. Opt. 2012;17(7): doi: /1.JBO

Date of download: 6/25/2016 Copyright © 2016 SPIE. All rights reserved. SERS bandpass images of 40 pM SERS-active AuNPs in solution. (a) White light image showing the laser irradiation volume, and (b) to (d) SERS bandpass images centered at 890, 900, and 910 nm, respectively; excitation at 785 nm with 1 s integration time per image. Circle indicates the laser illumination spot. Figure Legend: From: Filter-based method for background removal in high-sensitivity wide-field-surface- enhanced Raman scattering imaging in vivo J. Biomed. Opt. 2012;17(7): doi: /1.JBO

Date of download: 6/25/2016 Copyright © 2016 SPIE. All rights reserved. (left) SERS spectrum illustrating the filter passbands used to isolate the SERS signal (solid) from background (dashed). (right) (a) white-light image of 40 pM SERS-active and control AuNPs on filter paper, (b) 900 nm image showing signal from both samples, (c) linear background-subtracted image demonstrating improved SBR (1.7 to 9.1), and (d) background-subtracted signal shown in fasle color superimposed on the white light image; excited simaltaneously at 785 nm with 1 s image integration. Figure Legend: From: Filter-based method for background removal in high-sensitivity wide-field-surface- enhanced Raman scattering imaging in vivo J. Biomed. Opt. 2012;17(7): doi: /1.JBO

Date of download: 6/25/2016 Copyright © 2016 SPIE. All rights reserved. In vivo imaging of SERS AuNPs subcutaneously at different concentrations in dorsal mouse skin: (a) white-light images, with arrows indicating AuNP injection sites; (b) 900-nm bandpass SERS images showing poor SBRs (1.1, 0.98, and 0.92 at 80, 40, and 20 pM, respectively); (c) background-subtracted images demonstrating improved SBRs (905, 356, and 9.3 at 80, 40 and 20 pM, respectively) and visualization even as low as 20 pM AuNPs; 785-nm excitation with 3 s image integration in all cases. Figure Legend: From: Filter-based method for background removal in high-sensitivity wide-field-surface- enhanced Raman scattering imaging in vivo J. Biomed. Opt. 2012;17(7): doi: /1.JBO