Heart profile

Human heart What Is the Heart? the heart is a muscular organ that pumps blood to the body. heart is at the center of the circulatory system. An electrical system controls the heart and uses electrical signals to contract the heart's walls. When the walls contract, blood is pumped into the circulatory system. Inlet and outlet valves in the heart chambers ensure that blood flows in the right direction.

Human heart. the heart is vital to our health and nearly everything that goes on in our body. Without the heart's pumping action, blood can't move throughout your body. healthy heart supplies our body with the right amount of blood at the rate needed to work well. If disease or injury weakens our heart, the body's organs won't receive enough blood to work normally.

Heart structure the circulatory system or cardiovascular system consist of the heart and blood vessels, the heart is the key organ in the circulatory system. The volume of the heart like size of our fist is almost located between the lungs on the left side. 1)Heart contains four hollow spaces called champers: left atrium the right atrium the left ventricle the right ventricle

Heart structure 2)blood vessel: a vessel is defined as a hollow container for carrying something: a cup, a bucket, a tube. Blood vessels, then, are hollow container for carrying blood. Located throughout the body. There are three varieties of blood vessels: arteries, veins, and capillaries. During blood circulation, the arteries carry blood away from the heart. The capillaries connect the arteries to veins. Finally, the veins carry the blood back to the heart.arteriesveinscapillaries

heart function the primary function of the heart is to pump blood throughout the body. The regular and continuous contracting of heart muscle, myocardium, generates and sustains an arterial blood pressure necessary to provide adequate perfusion of organs.

heart profile The major heart test that do on the clinical laboratory is 1)lactate dehydrogenize(LDH) 2) creatine kinase (ck. ck-mb) 3)tropinin 1

Lactate dehydrogenase This enzyme catalyzes the reversible reaction between pyruvic and lactic acids. The lactate dehydrogenase (LDH) test measures the amount of LDH in the blood LDH is most often measured to check for tissue damage. The enzyme LDH is in many body tissues, especially the heart, liver, kidney, skeletal muscle, brain, blood cells, and lungs so LDH can be found in many tissues in the body, total LDH is not specific for heart disease.

Lactate dehydrogenase LDH exists in 5 forms, which differ slightly in structures LDH-1 is found primarily in heart muscle and red blood cells. LDH-2 is concentrated in white blood cells. LDH-3 is highest in the lung. LDH-4 is highest in the kidney, placenta, and pancreas. LDH-5 is highest in the liver and skeletal muscle. All of these can be measured in the blood A typical range is IU/L

Creatine Kinase – CK enzyme present in high concentration in the cytoplasm of myocytes and is the most widely used enzyme for evaluation of neuromuscular disease. In muscles, this enzyme functions by making ATP available for contraction by the phosphorylation of ADP from creatine phosphate by catalyzing the reversible phosphorylation of creatine by ATP to form phosphocreatine + ADP. Phosphocreatine is the major storage form of high energy phosphate in muscle

Creatine kinase There are four principle isoenzymes of CK:- 1-CK-1: BB isoenzyme, found mostly in the brain 2-CK-2: MB isoenzyme, highest concentration in cardiac muscle, with lesser amounts in skeletal muscle 3-CK-3: MM isoenzyme, found in skeletal muscle, with lesser amounts in cardiac muscle 4-CK-Mt: Found in mitochondrial membranes, and comprises 15% of total cardiac CK activity.

Creatine kinase-mb The creatine kinase-MB fraction (CK-MB) is part of total CK and more specific for cardiac muscle that other striated muscle. It tends to increase within 3 to 4 hours of myocardial necrosis, then peak in a day and return to normal within 36 hours. It is less sensitive than troponins Total Creatine Kinase (Total CK)Normal Value: ng/ml Creatine Kinase-MB (CK-MB)Normal Value:0-3 ng/ml

Troponin 1 Troponin I and T are structural components of cardiac muscle. They are released into the bloodstream with myocardial injury. They are highly specific for myocardial injury--more than CK-MB--and help to exclude elevations of CK with skeletal muscle trauma. Troponins will begin to increase following within 3 to 12 hours, about the same time frame as CK-MB Normal Value:<0.4 ng/ml

Lactate dehydrogenase LDH is most often measured to check for tissue damage. The enzyme LDH is in many body tissues, especially the heart, liver, kidney, skeletal muscle, brain, blood cells, and lungsenzyme A typical range is IU/L (international units per liter Lactate dehydrogenase is an enzyme that catalyzes the conversion of lactate to pyruvate. It is not tissue-specific, being found in a variety of tissues, including liver, heart and skeletal muscle

Elevated LDH levels Elevated LDH levels : 1- Hemolysis; serum has higher levels than plasma as the enzyme is released from cells during clotting.. 2- Physiologic: Exercise 3-Liver disease: Increased LDH1 and LDH2 4-Muscle disease: Increased LDH5.

L actate dehydrogenase Higher-than-normal levels may indicate 1- Heart attackHeart attack 2-Hemolytic anemiaHemolytic anemia 3-Liver disease (for example, hepatitis)Liver diseasehepatitis 4-Low blood pressure 5- Muscle injury 6-PancreatitisPancreatitis 7-Tissue dead

Hemolytic anemiaanemia Hemolytic anemia is a condition in which there are not enough red bloodanemia cells in the blood, due to the premature destruction of red blood cells Causes Hemolytic anemia occurs when the bone marrow is unable to increase production to make up for the premature destruction of red blood cells.).

Creatine Kinase – CK CK has a very short half life, < 1 hour in horses. Activity increases quickly (peaks at 6-12 hours) and returns to normal within hours Total Creatine Kinase (Total CK)Normal Value: ng/ml Creatine Kinase-MB (CK-MB)Normal Value:0-3 ng/ml The creatine kinase-MB fraction (CK-MB) is part of total CK and more specific for cardiac muscle

Causes of increased CK 1-Physiologic: CK values in young puppies are higher than in adults 2-· Muscle disease: Detection of increased activity in serum is useful as an indicator of muscle injury. 3-Artifact: Hemolysis will increase CK values as analytes in red blood cellsor their membranes (adenylate kinase) contribute to the enzymatic reaction for CK, artifactually increasing values.

Creatine Kinase Creatine Kinase (CK): Unexpectedly High Asymptomatic high CK: General – More common in males than females – May occur at all ages

heart attack A heart attack occurs when blood flow to a part of your heart is blocked for a long enough time that part of the heart muscle is damaged or dies. caused by a blood clot that blocks one of the coronary arteries. The coronary arteries bring blood and oxygen to the heart. If the blood flow is blocked, the heart is starved of oxygen and heart cells die

Creatine Kinase (CK): Low Muscle disease Hyperthyroidism Multiorgan failure Rheumatic diseases: Active inflammation

Troponins Troponin Normal Value:<0.4 ng/ml highly specific for myocardial injury-more thanCK-MB Troponins will begin to increase within 3 to 12 hours, about the same time frame as CK-MB

Ischaemic Heart Disease schaemic Heart Disease, otherwise known as Coronary Artery Disease, is a condition that affects the supply of blood to the heart. The blood vessels are narrowed or blocked due to the deposition of cholesterol plaques on their walls. This reduces the supply of oxygen and nutrients to the heart musculature, Ischemic heart disease is caused by an imbalance between the myocardial blood flow and the metabolic

Laboratory Procedure Manual Analyte: Lactate Dehydrogenase Matrix: Refrigerated Serum Method: Beckman Synchron LX20

TEST PRINCIPLE The LX20 with LD reagent (using lactate as substrate) utilizes an enzymatic rate method to measure LD activity in biological fluids. In the reaction, the LD catalyzes the reversible oxidation of L-Lactate to Pyruvate with the concurrent reduction of ß-nicotinamide adenine dinucleotide (NAD) to ß-nicotinamide adenine dinucleotide hydroxylase (reduced form; NADH). The system monitors the rate of change in absorbance at 340 nm over a fixed time interval. The rate of change in absorbance is directly proportional to the activity of LD in the sample

references ml ml _dehydrogenase.pdf

By: rahaf babgi Alaa alamoudi