Facilitating tissue collection for translational research Jane Rogan Business Manager

MCRC Biobanking: The Basic Concept Make quality tissue collection and retrieval easy for the researcher Regulatory Framework Sample Collection Logistics Sample Quality Informatics Sample Access

Collaborating Trusts MCRC Biobank Pennine Acute Hospitals Trust The Christie NHS Foundation Trust South Manchester NHS Foundation Trust Salford Royal NHS Foundation Trust HPB Breast Colorectal Skin Urology Gynaecology Haematological Malignancies Lung Breast Head & Neck Colorectal Breast Upper GI Urology Neurology

Building a successful Biobank

Donations from over 6000 patients

Donations from 400 patients

Biobank Project Applications Total Applications: 86 Total Approved: 75 Average Approval Time: 30 working days (median)

The “Six-Pack” 1.Fresh Frozen Diseased Tissue 2.Fresh Frozen Non-involved Tissue 3.FFPE Disease Tissue 4.FFPE Non-involved Tissue 5.Pre-operative Blood sample 6.Pre-operative Urine sample

Melanoma Tissue Collection Pathway Collect Fresh From Theatres Fresh Cut-Up in Pathology Diagnostic Sample Research Sample Mouse model of tumour Frozen Tissue for Nucleic Acid Extraction Fixed Tissue for Staining

Evolving the Collections RequirementConsiderations Prospective Fresh Tissue Availability of tissue Staff available for sample delivery Media for keeping samples viable Use for Animal Models Specific consent for animal models Size of tumour tissue Alternative Sample Types (fluids, plucked hair) Routes / logistics of sample collection Serial Blood Collection inc CTCs Biobank Technician presence in clinic Past samples (e.g. pre- treatment/primary tumours) Specific consent Requesting blocks from local hospitals Sampling tumour in different areas Further input from pathology Clinical Trial Sample Collection Space for sample storage Different protocols for each trial

Biobank Projects by Type Total Approved Projects By Type Active Projects By Type (Total 33*)

Tissue Microarrays Current disease areas include prostate, bladder, lung Link to clinical databases Recently obtained Section 251 Support which allows the Biobank to link archival samples to clinical data Support through Surgical Oncology Research Theme – Data Manager employed

External Projects utilising Biobank Resource Stratified Medicine Programme – Phase 2 –Lung focus –Biopsy samples from late stage patients 100k Genome Project –Lung, Colorectal, Prostate TracerX –Facilitating sample collection MesoBank –Mesothelioma

Research Biopsies Working with clinical teams to introduce sample collection as a research only procedure How tumours change over time Comparing primary and metastatic tumours in patients Research into why patients are relapsing on targeted treatments Pilot to being in Malignant Melanoma

Key Success Drivers Established network model with existing infrastructure and pathways for sample collection Excellent clinical engagement across all cancer disease types Robust governance framework for tissue and data Proven track record of providing quality samples Research needs drive the sample collection, not the other way round

Thank you