The Total Mycotoxin Inactivator UltraSorb ®

STRENGTH OF UltraSorb ® : Scientific criteria supporting Scientific criteria supporting the superiority of the product

Unique Composition, combining - Hydrated Alumino Silicates - Mannan Oligo Saccharides - Organic acids - Activated Charcoal

Effects of Unique Composition Effects of Unique Composition Hydrated Alumino Silicates Purpose : attraction and captation of toxines Effects : better growth + better muscle gain

Effects of Unique Composition Effects of Unique Composition Mannan Oligo Saccharides (MOS) Purpose : adhesion pathogenic bacteria and stimulation mucosal immunity Effects : improvement overall intestinal health

Effects of Unique Composition Effects of Unique Composition Organic Acids Purpose : creating an acid environment means less growth/adhesion of pathogenic bacteria Effects : improvement overall intestinal health + more weight gain

Effects of Unique Composition Effects of Unique Composition Activated Charcoal Purpose : absorption of harmful and undesirable substances Effects : improvement overall intestinal health + more weight gain

2) Look at net efficacy = adsorption - desorption 3) Activity spectrum besides aflatoxins : vomitoxin, ochratoxin, zearalenone, T-2 zearalenone, T-2 4) No nutrient binding at maximum dosage recommended for binding mycotoxins in feed binding mycotoxins in feed 1) High binding capacity at recommended dosage rate : supported by scientifically validated test procedure at dosage used in practise 5) Data on ‘in vivo” effect (cfr. Dr. Nick Dale, 1998) 7) Lowest cost in correct dosage range 6) Totally safe (heavy metals, dioxins, pcb’s) Scientific criteria supporting UltraSorb ®

test at 1.5 Kg/ton Test done by Dra. Sala de Miguel - Argentina (lotnbr : 0263) In vitro adsorption/desorption - Official Method of A.O.A.C. (HPTLC) - method with pepsin

Net efficiency: test at 1,5 Kg/ton Binding of Mycotoxins by UltraSorb ® Test done by Dra. Sala de Miguel- Argentina In vitro adsorption/desorption - Official Method of A.O.A.C. (HPTLC) - method with pepsin

Test done by Dra. Sala de Miguel - Argentina (lotnbr : 0263) In vitro adsorption/desorption - Official Method of A.O.A.C. (HPTLC) - method with pepsin test at 1,5 Kg/ton Binding of Mycotoxins by UltraSorb ®

Experiments at INTA - Instituto Nacional de Technologia Agropecuaria, Pergamino B.A.) 2 groups of 80 birds (ranking per 1 gram initial weight difference) : summer conditions (25-30°C) FCR 1,95 1,93 UltraSorb ® No nutrient binding

Minimum four experimental treatments are necessary : Diet 1 - control diet, with neither aflatoxin nor the binding agent ; Diet 2 - which is Diet 1, but containing aflatoxin ; Diet 3 - which is Diet 1, but containing the binder ; and Diet 4 - which is Diet 2 containing aflatoxin and binder. A test tube can never duplicate the conditions of the digestive tract of the animal. These should then be further evaluated in in vivo tests. Mycotoxin binders: Now it is time for real science

Experiments at INTA - Instituto Nacional de Technologia Agropecuaria, Pergamino B.A. Combination of the results from two experiments. Relative value to negative control Without toxins contamination UltraSorb ® does not bind valuable nutrients With toxins contamination UltraSorb ® reduces detrimental effect of aflatoxins Efficacy of UltraSorb ® with and without toxins in the feed

Analyse UltraSorb Legal Limits mg/kg mg/kg Zn : 4,6 --- Cd: 0,4 5,0 Pb : 4,030,0 Cr : 1,0 --- As : 0,9 12,0 Hg: ? --- Analysis RIKILT Institute Netherlands : UltraSorb ® is NEGATIVE on DIOXINS UltraSorb ® and heavy metals: level - legislation

10 piglets : 5 x 2 : Control clean feed = 181 ppb DON ppb ZEA; Contaminated feed = 1600 ppb DON ppb ZEA; UltraSorb ® 2 kg/ton, GENT – Belgium DON toxin in blood (ppb) a c ab a’ c’ b’ Piglet trial with UltraSorb ® - Mycotoxins in blood Screening test (Hobu/IWT project 2004)

10 piglets : 5 x 2 : Control clean feed = 181 ppb DON ppb ZEA; Contaminated feed = 1600 ppb DON ppb ZEA; Mercolite Xtra Forte ® 2 kg/ton, GENT – Belgium ZEA toxin in blood (ppt) a c b a’ c’ b’ Piglet trial with UltraSorb ® - Mycotoxins in blood Screening test (Hobu/IWT project 2004)

Product µg TEQ Condition Ade 6.1 Milbond 75.1 Zeotek 4.92 Myco Ad 7.0 Flobond 27.0 ULTRASORB ® 0.83 Tests done by Xenobiotic Detection Systems inc. (George Clark) More sensible & reliable (Calux) Bioassay at RIKILT (NL) give : NEGATIVE for UltraSorb ® Banned in E.U. DIOXINS (+PCB’s) in Some Commercial Mineral Binders

Source : Agil-Website : march 2001 SORBATOX : 0,44 pcg/gram Condition ADE : 1,30 pcg/gram Maximum for kaolonitic clays = 0,5 pcg / gram (analytical blank : 0,31 pcg/gram - Lab in UK) UltraSorb ® : negative (Calux bio-assay RIKILT-NL - GC/MS) Dioxine levels in SORBATOX and CONDITION Ade

DON in feedFeed Conversion DON in bile % Control UltraSorb ® Experimental Farm - France - Germany / April 2009 In vivo trial with UltraSorb ® on DON in Piglets DON contamination in UltraSorb ® group was more than TWICE the level in control, but no significant difference in performance (FI, DWG, FCR). UltraSorb ® bound the excess of DON (bile data)

Feed Conversion 1,42 1,44 1,46 1,48 1,5 1,52 1,54 1,56 1,58 1,6 1,62 ControlUltraSorb ® (1,5 Kg/T) FCR at 28 days Experimental Farm - Poland - September 2010 UltraSorb ® at 1,5 Kg/T significantly improved FCR in broilers fed with T-2 contaminated feed compared to positive control (commercial toxin binder) - 7% In Vivo trial: Mercolite Xtra Forte ® on T-2 in Broilers

The most economical way for toxin binding starts here Mercordi Animal Care Stadsbeemd 1215 Halen - Belgium Tel.: Fax: UltraSorb ®