PAGE, Analyzing proteins & Evolution. Timeline: Monday 1/28: Lecture Tuesday 1/29: Pre-Lab Quiz, Run Gel, Stain and De- stain (someone plan to stay late)

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Presentation transcript:

PAGE, Analyzing proteins & Evolution

Timeline: Monday 1/28: Lecture Tuesday 1/29: Pre-Lab Quiz, Run Gel, Stain and De- stain (someone plan to stay late) Thursday: Analyze Gels

Prep. For lab dilute stains and buffers ice? boiling water bath

PAGE: Polyacrylamide Gel Electrophoresis Separates Proteins by size or charge Used to estimate size of DNA/RNA fragments DNA loaded into the well will run positive through the pores of the Polyacrylamide Gels Sorts DNA according to length Smaller strands will run longer because they can fit through more pores in the gels than bigger strands of DNA The Gel can be seen under UV light or stained with Ethidium Bromide to be seen with the human eye

Analyzing Proteins: PAGE can provide: Size of DNA molecules after restriction enzyme digestion Genetic Fingerprinting Desired protein was properly isolated Base Pair Size Whether food is Genetically Modified What the Relationship is between species (through shared base pairs)

Evolution PAGE Analysis: Help clarify the connections between species how they are related Evolution of fish species The genes that connect them The differences in genetic material due to surroundings

Polyacrylamide gels Tutorial 23 and 25 from disc.