ShCon shNaa10p GAPDH MCL1 EPHB4 RPS6KA5 SMAD1 BFAR EIF4B CYR61 STAT5B NFkbil2 VEGFC TNFRSF1A CyclinD Supplementary Figure S1 RKO cells A B siCon siNaa10p-395siNaa10p-464siNaa10p-649.

Slides:

Advertisements

Similar presentations

25 A hMLH1 B Acetylated H3 Enrichment SW480 RKO Position from +1 BBII ++-- SW480 BIBI ++-- RKO C hMLH1 GAPDH Ab D Methyl-H3-K4 enrichment Position.

Advertisements

Suppl. Figure 1 A B Suppl. Figure 1. Relative (A) miR-21 and (B) miR-224 expression levels in HCC tumors in different stages (I,II,III,IV) assessed by.

Ct log DNA （ pmol) P1P2 Supplementary Fig. S1 Standard curve of the PCR amplification efficiency of transcripts.

Supplementary Figures. Figure Legends Suppl. Figure 1. Flow cytometry analysis to monitor apoptosis levels in MCF-7 cells. As control MCF-7 cells grown.

Normal skin tissue Supplementary Fig 1A KLF4 has a tumor suppressive function in human skin cancer. Skin cancer tissues were deparaffinized and IHC was.

*** * * * Figure S1 Supplementary Figure 1. Analysis of JARID1A–D mRNA levels showed that JARID1D knockdown had insignificant or weak effects on expression.

Supplementary figure 5.

Supplementary Figure 1. Generation of hepatocyte-specific A20 knockout mice. Targeting scheme. Diagram showing the LoxP-flanked (Floxed) and the deleted.

From: Lentiviral Vector-Mediated PAX6 Overexpression Promotes Growth and Inhibits Apoptosis of Human Retinoblastoma Cells Invest. Ophthalmol. Vis. Sci..

From: Genetic Polymorphisms in the Promoter of the Interferon Gamma Receptor 1 Gene Are Associated with Atopic Cataracts Invest. Ophthalmol. Vis. Sci..

Chemotherapy and Cancer Stem Cells

Novel Cis Element for Tissue-Specific Transcription of Rat Platelet-Derived Growth Factor β-Receptor Gene by Yasunori Takata, Yutaka Kitami, Tomikazu Fukuoka,

A B Supplementary figure S3 ShCtl ShLCoR LCoR mRNA (%) Optical density

Smad3 reduces susceptibility to hepatocarcinoma by sensitizing hepatocytes to apoptosis through downregulation of Bcl-2 Yu-An Yang, Gen-Mu Zhang, Lionel.

Volume 135, Issue 5, Pages e2 (November 2008)

A B C Supplementary figure S7

A Requirement for ZAK Kinase Activity in Canonical TGF-β Signaling

Volume 138, Issue 1, Pages e3 (January 2010)

Supplementary Figure 1 A B C siControl siRNF31 siRNF31+siP53 siControl

Metastasis gets site specific

Constitutive and tumor necrosis factor-α-induced activation of nuclear factor-κB in adenomyosis and its inhibition by andrographolide Bin Li, M.S., Ming.

A Requirement for ZAK Kinase Activity in Canonical TGF-β Signaling

A B HT29 HCT15 Colo205 Ls174T LS180 Isotypic control

Prognostic Significance of TAZ Expression in Resected Non-Small Cell Lung Cancer Mian Xie, MD, PhD, Li Zhang, MD, Chao-Sheng He, MD, Jin-Hui Hou, MD,

Volume 138, Issue 2, Pages (February 2010)

Volume 138, Issue 1, Pages e3 (January 2010)

FLAG β-actin p53 SET M.w. kDa D β-actin p53 G9a

Prognostic Impact of CD204-Positive Macrophages in Lung Squamous Cell Carcinoma: Possible Contribution of Cd204-Positive Macrophages to the Tumor-Promoting.

Chandra Goparaju, PhD, Jessica S

Supplementary Figure S1: FGFR4 silencing synergistically enhances the effects of chemotherapy in colon cancer cells. siFGFR4 FGFR4_6 FGFR4_2 Neg Con FGFR4.

Supplementary Figure 2. Effect of IL-32 and siRNA of IL-32 on melanoma, colon and prostate cancer cell growth and apoptosis as well as IL-32 expression.

Differential Modulation of Ku70/80 DNA-Binding Activity in a Patient with Multiple Basal Cell Carcinomas Paola Mazzarelli, Carla Rabitti, Paola Parrella,

AbH AbF p=0.01 p= p=0.004 p=0.001 Fold increase in 4

Inappropriate Gene Activation in FSHD

Molecular Therapy - Nucleic Acids

a b AP-1 Sp1 Nuclear extract Competitor (100X) CDCA 50 µM + - M

Supplementary Information 1

Integrative Functional Genomics Implicates EPB41 Dysregulation in Hepatocellular Carcinoma Risk Xinyu Yang, Dianke Yu, Yanli Ren, Jinyu Wei, Wenting.

mRNA level as % relative to

Histone deacetylase 1 protein depletion affects general histone acetylation and specific gene expression. Histone deacetylase 1 protein depletion affects.

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Estradiol suppresses type I collagen synthesis in mesangial cells via activation of activator protein-1 Sharon Silbiger, Jun Lei, Joel Neugarten Kidney.

Xiangmei Chen, Wenhu Liu, Jianzhong Wang, Xiaodan Wang, Zhiheng Yu

“The Best-Laid Plans … Often go Awry …”

Leslie A. Bruggeman, Scott H. Adler, Paul E. Klotman

Fold Change of hsa-miR-3687 (T/N)(log2)

JNK Regulates Autocrine Expression of TGF-β1

Connective Tissue Growth Factor: Expression in Human Skin In Vivo and Inhibition by Ultraviolet Irradiation Taihao Quan, Tianyuan He, Sewon Kang, John.

LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition Huazhang Wu, Ying.

HDAC11 regulates CCL2 expression by recruiting PU.1.

Klotho is a target gene of PPAR-γ

STAT5a/PPARγ Pathway Regulates Involucrin Expression in Keratinocyte Differentiation Xiuju Dai, Koji Sayama, Yuji Shirakata, Yasushi Hanakawa, Kenshi.

Expression of SENP2 mRNA is regulated by palmitate-induced NF-κB activation. Expression of SENP2 mRNA is regulated by palmitate-induced NF-κB activation.

Volume 2, Issue 2, Pages (August 2012)

Supplemental Figure 1 Cell proliferation (% of mock)

Volume 2, Issue 4, Pages (October 2002)

Bismarck Amoah-Apraku, Mao-Zhong Fang, Nicolas J. Guzman, M.D

Ctip2 directly regulates Notch1 expression in differentiated cells.

A B D C E F Supplementary Figure S4. Epping et al. IP: flag input IgG

Activating mutation of Ras is associated with CDCP1 expression in NSCLC. A, NSCLC cell lines with Ras + B-Raf mutations and wild-type Ras were examined.

IL6 mRNA is not detected in metastatic prostate cancer cells.

A, GP and GR epithelial tumor cells are equally responsive to gefitinib. A, GP and GR epithelial tumor cells are equally responsive to gefitinib. Equal.

Greater induction of apoptosis following EGFR TKI treatment correlates with higher basal BIM expression across a panel of EGFR-mutant lung cancers. Greater.

TDP1 and TOP1 protein levels correlate positively with TDP1 and TOP1 mRNA levels in colorectal cancer cell lines. TDP1 and TOP1 protein levels correlate.

Supplementary Figure S5

Interaction of Cdx1 and Cdx2 with the putative Cdx-binding sites of the human DSC2 promoter. Interaction of Cdx1 and Cdx2 with the putative Cdx-binding.

BHLHB2 is part of a protein–DNA complex associated with the proximal E-box element of Bdnf promoter 4. BHLHB2 is part of a protein–DNA complex associated.

A B C Name Sequence TIMP3 promoter

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

Presentation transcript:

shCon shNaa10p GAPDH MCL1 EPHB4 RPS6KA5 SMAD1 BFAR EIF4B CYR61 STAT5B NFkbil2 VEGFC TNFRSF1A CyclinD Supplementary Figure S1 RKO cells A B siCon siNaa10p-395siNaa10p-464siNaa10p-649 RKO cells Relative mRNA levels

ShConshNaa10pshConshNaa10pshConshNaa10p inputIgGanti-p65 MCL1 promoter ChIP MCL1 promoter inputIgGanti-Naa10p MCL1 promoter siConsip65siConsip65siConsip65 inputIgGanti-Naa10p ChIP A D B Supplementary Figure S2 C MCL1 Probe Naa10p antibody RKO nuclear extract MCL1 probe (200X Cold) IgG +++ － + －－－－－ + － + － + －－－－－ MCL1 probe (400X Cold) －－－ + －－－－－ Free probe Band shift Supershift q C H I P o f M C L 1 p r o m o t e r ( F o l d c h a n g e o f I g G ) IgGNaa10p IP:

A C B Supplementary Figure S3 Relative Naa10p Expression Normal (n=5) Tumor (n=43) Relative Naa10pmRNA Expression Correlation between Naa10pand p65 in lung cancer tissues r=0.277 p=0.056 Naa10p p 6 5 Correlation between Naa10pand MCL1 in lung cancer tissues r=0.467 p<0.001 Naa10p M C L 1 Relative MCL1 mRNA Expression Relative p65mRNA Expression Relative MCL1 Expression Relative p65 Expression Correlation between MCL1and p65 in lung cancer tissues r=0.535 p<0.001 MCL1 p 6 5