Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the acid fast.

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Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the acid fast and endospore stains Review the structure and function of endospores

Modified Kinyoun’s Acid-fast Stain – Cold Method Heat fix smear Primary stain: Carbol fuchsin for AFB, (5 min) Rinse with water Decolorize briefly (~10 drops) with acid alcohol (~ 30 sec) Immediately rinse with water Counterstain: Methylene blue (1 min) Rinse with water

For Acid Fast Bacteria Use only Carbol fuchsin for AFB!! Acid Alcohol Methylene Blue

Student slide from SS 2015

Endospore Stain Heat fix smear Primary stain: Malachite green, steam (5 min) Rinse thoroughly with water (~ 30 sec) Counterstain: Safranin (1 min)

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