Molecular Axon Guidance PC12 cell…. then NGF added NGF is neurotropic and neurotrophic.

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Presentation transcript:

Molecular Axon Guidance PC12 cell…. then NGF added NGF is neurotropic and neurotrophic

Growth FactorsGuidance Factors

Netrin..was one of the first neurotropic factors

Findings

Netrin from FP

Netrin from FP, But perpendicular

Netrin in media

Cell locomotion like GCs Cell moves forward by extending filipodia & lamellipodia Focal adhesions are formed Cell is pulled forward

DiffusibleContact dependent

Extracellular matrix

Proteins of ECM - collagen

Extracellular matrix Proteins of ECM - proteoglycans

Extracellular matrix Proteins of ECM - fibronectin

Extracellular matrix Proteins of ECM - laminin collagen laminin

Interaction of cells with ECM inactive INTEGRINS

Interaction of cells with ECM INTEGRINS functions of outside-in signals differentiation motility growth survival ligands have RGD motif

Interaction of cells with ECM FOCAL ADHESIONS FA

Interaction of cells with ECM FOCAL ADHESION S

Interaction of cells with cells CELL ADHESION MOLECULES

Interaction of cells with ECM and other cells CELL ADHESION MOLECULES VS ECM ATTACHMENT MOLECULES

Tcf/Lef P E-Cadherin  -catenin p120 P Wnt Dsh Frz II Extracellular Space actin filaments  -catenin Ca 2+ Gsk3  APC Axin

Focus is often on adhesive function: Cadherins as molecular glue Cadherins as Master Regulators of Morphology Cadherins Actin Cytoskeleton  -catenin Rho-GTPases Arp2/3 Proliferation CDKI: p27/p21 Wnt Motility Passive Inhibition Rho-GTPases Active Promoter Adhesion AJ’s Type of Cadherin Stabilize others junctions Receptor Signaling EGF FGF PDGF Transcriptional/ Nuclear Role  -catenin/Wnt P120/kaiso In nucleus

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