1. New targets since 7/22/05: Tbru015978AAADihydroorotate dehydrogenase Chunks since 6/17/05: Lmaj002537BAIProtein tyrosine phosphatase Lmaj002537BAFProtein tyrosine phosphatase Targets dropped since 6/13/05: Lmaj012767AAA (49% seq ID to solved structure) Requested re-purification: Lmaj012795AAA (TA) Pviv005676AAA (MH) Tbru003034AAA (TA) Tcru006532AAA (MH) Crystal Structures - Unit 1

2. StatusTargetFunction SolvedLmaj003965AAAHypothetical Tbru019101AAAPutative riboflavin kinase Tbru020260AAAHypothetical RefiningLmaj006129AAHHypothetical Lmaj006873AAAHypothetical End of the pipeline

3. StatusTargetFunction Lack phasing powerLmaj00884AAAHit-family protein Lmaj002537AAAProtein tyrosine phosphatase Lmaj004542AAAMevalonate kinase Pfal008434AAARibose 5-phosphate epimerase Lmaj012795AAAHypothetical Diffract poorlyLbra003107AAAHypothetical Lmaj00592AAANADP dependent alcohol dehydrogenase Lmaj003759AAAHypothetical (was DNA binding protein) Lmaj012767AAAProteasome activator protein Lmaj013098AAAHypothetical Pfal006597AAAUbiquitin conjugating enzyme E2 Pviv005676AAAAdenosine deaminase Lbra003107AAAHypothetical Tbru003034AAAAcetyltransferase Tbru016838AASHypothetical Tcru006532AAAHypothetical Tbru018269AAAHypothetical Tbru013996AAAHypothetical SeMet in progressTcru011138AAAUbiquitin conjugating enzyme E2 Pviv009166AAAPhosphatidylethanolamine binding protein In the pipeline

4. Lmaj006129AAA 1.6Å PDB ID code 2AR1 Problematic target –No Se incorporation –No HA derivatives –Sacrificed several crystallographers Phases obtained from Lmaj006129AAHSeMet chunk Initial trace from fully automatic Elves program MR of chunk model into parent data Thanks to Lori Anderson & Rochester PEP Dali found 1ZCE, Z score = 19 Hypothetical Protein Atu2648 Agrobacterium Tumefaciens Northeast Structural Genomics Target