2. WB page 50 - Enzymes are… Proteins Biological Catalysts (speed up rxns without being used up) Generally Specific (act on one type of compound) Either catabolic (ab = a + b) or anabolic (a + b = ab) Catabolism; CutAnabolism; Add

3. Enzyme names Enzymes are named by adding the suffix “-ase” to the substance they act upon. E.g. Lipase (act on lipid), maltase (act on maltose), urease (act on urea)

4. Enzyme Structure They have an active site which attracts the substrate(s) and position the substrates to promote a reaction

5. Lock and Key Hypothesis Usually only one type of substrate molecule will fit into the active site of an enzyme. Substrate = Key Enzyme = lock

6. Maltose is made of two glucose molecules bonded together (1). The maltase enzyme is a protein that is perfectly shaped to accept a maltose molecule and break the bond (2). The two glucose molecules are released (3). A single maltase enzyme can break in excess of 1,000 maltose bonds per second, and will only accept maltose molecules.

7. Induced Fit modelInduced Fit model – partially flexible enzyme The enzyme’s active site is not an exact fit for the substrate. The shape of the enzyme changes when the substrate bind to the active site.

8. How do enzymes work? Enzymes speed up rxns by lowering the activation energy - activation energy (A E ) is the energy required before a rxn can occur. [“jumpstarting” the reaction] - can also be explained by an energy hill graph  A E = energy needed to push the reactants over the energy barrier

9. Energy hill graph

10. DO NOW: Sketch this graph into your notebook

11. What affects how enzymes work?

12. Enzyme Cofactors Alter the shape of the enzyme’s active site slightly to make active site more ‘reactive’ Enables substrates to ‘fit’ that might not have been able to without the cofactor Cofactors can be ions or a vitamin e.g. amylase need Cl ion to convert starch to maltose e.g. vit B2 important coenzyme in cellular respiration. Organic cofactors = coenzymes