1. BioMEMS: Microfluidics Optimization & Cell Culture Maintenance D. Michael Ackermann, John Payne, Hillary Samples, James Wells Group # 23 Advisor: Franz Baudenbacher, PhD.

2. The Problem: MEMS bioreactor Design Bioreactor Design Bioreactor –Accurate nano/picoliter fluid delivery –Structure for cell culture Bioreactor Maintenance System Bioreactor Maintenance System –Temperature control –Metabolic Measurements –Photographic surveillance

3. The Bioreactor Cell culture structure: Research Defined Cell culture structure: Research Defined Fluid Delivery Fluid Delivery –Nutrient Delivery – Syringe Pumps –Drug/Toxin Delivery – MEMS pumps

4. Bioreactor: The Plan Literature ReviewFall Literature ReviewFall –Picoliter Pump Designs –Cell Culture Structure Training/Analysis of Current Designs Training/Analysis of Current Designs –Microfluidic Profiling (External Pumps) Design MEMS PumpJan Design MEMS PumpJan Implementation into Reactor Feb Implementation into Reactor Feb Analysis/Design Correction Mar Analysis/Design Correction Mar

5. BioReactor: Current Work MEMS Fabrication MEMS Fabrication –Expose and Develop Photo-resist –Pour PDMS onto template –Attach PDMS to slide, making channels

6. The Maintenance System Temperature Control Temperature Control Integrated Heating Block Provide CO2 Incubation Provide CO2 Incubation Maintain Viable pH Level Monitor Metabolic Activity Monitor Metabolic Activity pH, glucose levels, etc Real-time Photography Real-time Photography LabVIEW Image Acquisition Builder

7. Maintenance System: The Plan Cell Culture Background & Training Fall Cell Culture Background & Training Fall –NanoDelivery, Inc. Laboratory –Splitting, re-Suspension, Medium Change, Microscopy, etc Incorporate System Inputs Jan Incorporate System Inputs Jan Heater, CO2 Line, Injection scope Implement Measurement Acquisition Systems Jan Implement Measurement Acquisition Systems Jan Design Image Acquisition Program Feb Design Image Acquisition Program FebLabVIEW Real-Time photography -Cellular responses & Maintenance in Bioreactor Analysis Mar Analysis Mar Controlled Delivery

8. Current Work: Bioreactor Maintenance System Learning Background and Technique Delivery into bioreactor Grow Cells Maintenance for future generation cell line  Cell Splitting  Change medium

9. Total Integrated System BioReactor+ Maintenance System Controlled Environment Controlled Input Measurement System Controlled Environment Controlled Input Measurement System

10. Obstacles Communication with advisor Communication with advisor Website – access denied! Website – access denied! Training Requirements Training Requirements