1. Lauren O’Brien, Adrienne Campbell, Lewis Reis, Paul Stewart

2. Bioreactor Operation Main Factors Affecting Bioreactor Productivity Optimization Final Design Cost and Suppliers Alternative Reactors Question Period

3. Purpose: Growth of the hybridoma culture and production of monoclonal antibodies. Bioreactor volume: 18981 L Mab Production: 30.26 kg/batch

4. fermentation temperature dissolved oxygen content supplied nutrient waste product accumulation

5. Effect of Air Flow Rate on mAb Production Initial Air Flow Rate: 1187 kg/hr

6. Effect of Mass Flow Rate of Stream S-108 on Bioreactor Volume and mAb Production Initial Flow Rate of S-108: 10112 kg/hr Initial Medium Percentage: 1.8

7. Effect of Mass Flow Rate of Stream S-109 on Reactor Volume and mAb Production Initial Flow Rate of S-109: 1077 kg/hr Initial Medium Percentage: 15.7

8. ParameterValue Volume (L)22776.41 Mass flow rate of stream S-109 - input (kg/batch) 4000 Mass flow of stream S-112 - output (kg/batch)18044.93 mAb production (kg/batch)62.23

10. In Vivo Inject the hydridoma cells into the peritoneal cavity of a mouse. Hybridoma cells multiply and produce fluid (ascites) in its abdomen. The mice are then ‘tapped’ and the mAb rich fluid is removed Advantages Inexpensive, easy to use, familiar Usually produces very high mAb concentrations that often do not require further concentration procedures that can denature antibody and decrease effectiveness. Disadvantages Contaminating mouse proteins Can cause pain or distress Animal welfare issues

11. Semi-Permeable Membrane Based Systems Hollow Fiber Membrane System Cells are maintained in a compartment separated from the media reservoir by a selective membrane. Cells are perfused continuously with media, but are not exposed to shear. Advantages More effective for supplying nutrients and removing waste High-density cultures, and therefore higher monoclonal antibody concentrations compared to batch Disadvantages More complicated and expensive High-density culture methods require frequent monitoring