1. The Microscope

2.  Spectroscopic Microscope  Light – Compound (Bright field)  Fluorescent  Confocal  Electron- Transmission and scanning

3. Fluorescence Microscopy Confocal Microscopy

4. Illuminating system  Light source  Condenser lens- immediately below specimen  Iris diaphragm- controls amount of light

5. Imaging system  Objectives-4X, 10X, 40X  Ocular- lens to look through; monocular & binocular  Oculars magnify 10 times  Stage- place where specimen are mounted

6. Magnification  Magnification total= Mag obj X Mag ocu  Parfocal  Parcentered  Field of view

7. Stages of making a slide  Sectioning-blade  Mounting-(wet)- glycerin; water  Dehydration-alcohol  Fixing-formaldehyde; glutaraldehyde

8. Determining size of field  Micrometers  Ocular micrometer  Stage micrometer  FOV

9. Use of the micrometer Demonstration