1. Paul Selleck CSIRO Australian Animal Health Laboratory Hendra Virus – an update

3. Acute Equine Respiratory Syndrome

4. Hendra Virus CPE Livestock Industries

5. Vic Rail also ill

6. Hendra Virus IFA

7. Experimentally Infected Horses

8. No antibody in Queensland horses No antibody in domestic species  wildlife animal wide range of habitats travel long distance birds or bats? Search for the natural host

9. Pteropus Bats 4 Pteropus species in Australia Antibody found in all 4 species Up to 30% seroprevalence

10. Hendra Research Organisation of Paramyxovirus Genome 56 kd53 kd 41 kd68 kd 82 kd220 kd Hendra virus genome > 18 kb NDV Genome size of 15.2 kb NPPMFHNL V

11. Publicity of the first outbreak

12. The Nipah Outbreak Between 98/9 – 99/4: 265 human cases, 105 fatal Resulted in the culling of 1.1 million pigs Virus isolated from brains of fatal human case in 99/3 CDC confirmed Hendra-like virus using anti- HeV sera Pig-to-pig transmission via respiratory route No human-to-human transmission detected!!! Assays developed for Hendra used for Nipah

13. The Nipah Outbreak

15. Media Response CSIRO Family fears for boy whose dog has Hendra Owners of a dog that tested positive to Hendra virus hold fears for their son, 11, who shared a bed with Dusty, but say the family won't let their beloved pet be put down.

16. CSIRO THE owners of a dog that tested positive to the Hendra virus now hold grave fears for their young son. Two-year-old kelpie Dusty tested positive to virus anti-bodies earlier this week and authorities say the family pet had had contact with three horses that died from the virus. Owners Neil and Liz Fearon now say they are concerned for the health of their 11- year-old son who has slept with the dog in his bed. The owners have also said they will not euthanase the dog despite Queensland law requiring infected animals be put down. Media Response

17. IFA Based on M and NP Group Reactive VNT Based on G protein Hev and Niv specific but significant cross reaction ELISA Based on NP Group Reactive Expressed NP and G antigens under development PCR Originally based on M gene Now added NP and L gene PCR G gene PCR under development CSIRO Diagnostic Assays

18. CSIRO Thank you

19. CSIRO