1. Grab a lab packet and complete the pre-lab questions on the first page
9/24/15
Admit Slip
Grab a lab packet and complete the pre-lab questions on the first page
Date:
9/24/15
Topic:
Enzyme Lab
Page # ___

2. Today in Room 315… ADMIT SLIP: Complete the pre-lab questions
Date:
9/24/15
Topic:
Enzyme Lab
Page # ___
Today in Room 315…
ADMIT SLIP: Complete the pre-lab questions
HW: Write the Abstract on the back of the lab packet
CONTENT OBJ: SW investigate the effects of temperature, and substrate/enzyme concentration on the rate of enzyme activity
LANG. OBJ: SW write a lab protocol of their own creation
ANNOUNCEMENTS: Stay on top of your work!!
AGENDA:
Discuss admit slip
Write and carry out protocol
Complete Part B of lab
EXIT SLIP: Clean workspace!

3. Pre-Lab Questions
Brief discussion

4. Today’s Lab Hydrogen Peroxide
In today’s lab, we will be using chicken liver to investigate the reaction of catalase within the body. Liver cells (along with other cells in your body) produce the poisonous substance hydrogen peroxide as a by-product of cellular respiration. In order to keep the cells alive, the body uses an enzyme called catalase to break up the substance into harmless substances. This is the reaction diagram, which shows how catalase breaks up hydrogen peroxide (H202) into water (H20) and oxygen (O2).

5. Factors Affecting Reaction?
What factors could cause the rate of the reaction to change? (Increase or decrease)
Think about it: person eating a hamburger
What would cause him to eat (break down the meat) at a faster rate or slower rate?
Now what about the enzyme catalase?

6. Write these at the top of Page 2 in your lab packet!
Possible Experiments
Test the affect of…
-Temperature
-Substrate concentration (amount of hydrogen peroxide)
-Enzyme concentration (amount of liver)
…on the rate of enzyme activity
Write these at the top of Page 2 in your lab packet!

7. Materials List
Using the materials available and keeping the possible variables in mind, design an experiment!

8. Lab Protocol
As a group, pick one variable to test, and then design a protocol that allows you to test for that variable
Plan to use 4 test tubes (one as a control)
NOW: Fill out page two, write out your protocol, and check with me for the OK to get started!

9. Part B
Upon completing your own experiment, carefully follow the directions for Part B on the last page, and answer the questions accordingly!
When you have completely finished, make sure to clean up as described on the bottom of the last page!

10. Homework
Write an Abstract for your lab report using the last page of your lab packet

11. Catalase Reaction Admit Slip
Directions: Fill in the boxes with the components of the catalase reaction above.
Date:
9/25/15
Topic:
Enzyme Notes
Page # ___

12. Today in Room 315… ADMIT SLIP: Label components of catalase reaction
Date:
9/25/15
Topic:
Enzyme Notes
Page # ___
Today in Room 315…
ADMIT SLIP: Label components of catalase reaction
HW: Answer the apple question (last slide of ppt)
CONTENT OBJ: SW draw graphs of the relationship between enzyme activity and various factors
LANG. OBJ: SW speak with partners to determine best answer for in-class quiz
ANNOUNCEMENTS: Attendance…
AGENDA:
Review Lab
Enzyme Graphing
Open Note Quiz
EXIT SLIP: Paste HW into journal

13. Lab Overview
Testing the Factors that Affect Enzyme Function in Catalase

14.
Virtual Lab

15. http://www.mhhe.com/biosci/genbio/virtual_labs/ BL_11/BL_11.html
Virtual Lab

16. Bio 2 Only:
Worksheet: Factors Affecting Enzyme Activity (see website for download)
Follow this website:

17. Factors that Affect Enzyme Function*
You know three of them! What are they? pH
Salinity
Temperature
Activators
Substrate concentration
Inhibitors
Enzyme concentration

18. Enzyme Limits
Our results showed that as enzyme concentration increased, so did the rate of reaction
Does that trend go on indefinitely?
If we added TONS of enzyme, would the reaction just happen faster and faster and FASTER???

19. Enzyme Concentration*
What does this graph represent??

20. Enzyme Concentration
We know from our experiment that as enzyme  = the reaction rate 
More enzyme = more chances to collide with substrate
But eventually, what happens?
Reaction levels off, because not all enzymes can find substrate!

21. Substrate Concentration*
What about substrate concentration? Does the rxn rate increase go on indefinitely??
No, eventually the enzyme becomes saturated, meaning all enzymes are tied up with substrate

22. https://www.youtube.com/watch?v=0nwI0xnG8W 4
Effect of Temp & pH 4

23. Temperature*
What did the results from the experiment show, when testing cold, room temp, and heated liver?
Enzymes have an ideal temperature range, because they are proteins
When proteins get too hot, they denature (3D structure destroyed = unable to function), and the reaction stops

24. What are the names of ORGANISMS that live in thermal hot springs??
Different Ranges*
Extremophiles!
Different enzymes have different ideal temperature ranges

25. pH*
Just like temperature, enzymes have an ideal pH where they work best (ie the top of the graph)

26. Book Quiz Open Note Book Quiz
Read pages on Enzymes in MCAS Book
Answer questions in this format:
“1) A: It is a carbohydrate”
Book Quiz
Note: You do not have to write the question or explain your answer.
3. This is a 15 point quiz grade.
Therefore if you finish 2/3 of it, and get all those answers right, you will get a 10/15 or a 66%. That is a D.
4. You have until the end of the class to finish, but no additional time.
5. You may work in groups of 2 or 3, but no more. Choose partners WISELY!
6. When you are done, you should complete your homework. (See Ms. Gately for the slip)
Label a lined piece of paper (will be handed out) with your name, partners (if any) and the numbers 59-73
Your answers should be written as follows:
59) A. It is a carbohydrate
You do not have to write the question, but make sure to include the full answer (not just the letter).
This is a quiz- do not ask me for help! 

27. Homework