2. Transcription Definitely not Transformation, Transduction or Translocation!

3. What is a Gene? One Gene – One Enzyme One Gene – One Polypeptide One Gene – One RNA transcript A GENE IS A SEGMENT OF DNA THAT CODES FOR - - - - - - - - AN RNA TRANSCRIPT!!!!

4. GenerRNA Component of Ribosome tRNA Carries amino acids to Ribosome mRNA Codes for Polypeptide snRNA Helps to splice primary transcripts

5. Which of the following will get translated? A.rRNA B.tRNA C.mRNA D.snRNA 1.A only 2.B only 3.C only 4.D only 5.A & B 6.B & C 7.C & D 8.A, B, & C 9.A, B, C, & D

6. Which of the following are directly involved with translation? A.rRNA B.tRNA C.mRNA D.snRNA 1.A only 2.B only 3.C only 4.D only 5.A & B 6.B & C 7.C & D 8.A, B, & C 9.A, B, C, & D

7. What best represents rRNA? 1.Red 2.Blue 3.Green 4.Brown

8. What best represents tRNA? 1.Red 2.Blue 3.Green 4.Brown

9. What best represents snRNA? 1.Red 2.Blue 3.Green 4.Brown

10. Life is just an RNA SAMWICHH!

11. Hey Check out this basic transcription animation! http://www- class.unl.edu/biochem/gp2/m_biology/animat ion/gene/gene_a2.htm http://www- class.unl.edu/biochem/gp2/m_biology/animat ion/gene/gene_a2.htm l

12. What did the idiot scribe represent? 1.RNA primase 2.RNA polymerase 3.DNA polymerase 4.Reverse transctriptase 5.Helicase

13. What is/are the substrate (s) for RNA polymerase? 1.ATP, GTP, UTP, CTP 2.ATP only 3. d ATP, d GTP, d CTP, d TTP 4.ATP, GTP, CTP, TTP 5.Amino acids

14. How many RNA polymerases are there? THREE RNA Polymerase I – Transcribes nucleolar genes into rRNA RNA Polymerase II – Transcribes genes into mRNA (which eventually turns into a polypeptide) RNA Polymerase III – Transcribes genes into tRNA and snRNA

15. How does an RNA Polymerase “FIND” a gene to transcribe? TRANSCRIPTION FACTORS FIND A TATA BOX ON THE PROMOTER REGION!!!!!!!!!!!!!!!!!!!!

20. Check Out the Process in a MovEEE http://vcell.ndsu.edu/animations/transcriptio n/index.htm http://vcell.ndsu.edu/animations/transcriptio n/index.htm

21. What did Sir Spliceolot accomplish? 1.Cut out different DNA sections called introns. 2.Cut out different RNA sections called exons. 3.Cut out different RNA sections called introns. 4.Cut out different DNA sections called exons.

22. Why are introns called introns? They are INTERVENING noncoding sequences found within a gene!

23. Why are exons called exons? They are coding sections of a gene that get EXPRESSED as a component of a protein!

24. What really splices out the introns? 1.Cutterase 2.Dicerase 3.Spliceosome 4.Blitzenase 5.Rudolphase

25. What really is a SPLICEOSOME? snRNA small protein BIG PROTEIN snRNP SPLICEOSOME