1. Bacterial Infection of WoundBy
Dr. Humodi A. Saeed
Associate Prof. of Medical Microbiology
College of Medical Laboratory Science
Sudan University of Science and Technology
E mail address:

2. Bacterial Infection of Wound

3. The Wound
Definition
A wound is a breach (damage) in a normal tissue (May be superficial or deep) resulting accidentally or of planned surgery.

4. The Wound
Types
1. Early acute wound
2. Long-term chronic wounds

5. Bacterial Infection of Wound
Wound contamination
Wound colonization
Wound Infection

6. Bacterial Infection of Wound
Wound contamination
The presence of non-replicating bacteria in the wound.

7. Bacterial Infection of Wound
Wound contamination
All chronic wounds are contaminated by bacteria.

8. Bacterial Infection of Wound
Wound contamination
contaminants come from the indigenous microflora and/or the environment.

9. Bacterial Infection Wound
Wound colonization:
The presence of replicating bacteria adherent to the wound in the absence of injury to the host.

10. Bacterial Infection of Wound
Wound colonization:
Most of the following organisms are normal skin flora.
1. S. epidermidis,
2. other coagulase –ve Staphylococci.
3. Corynebacterium sp.
4. Brevibacterium sp.
5. Proprionibacterium acnes

11. Bacterial Infection of Wound
Wound Infection
The presence of replicating bacteria within a wound that cause host injury.

12. Bacterial Infection of Wound
Wound Infection
Staphylococcus aureus,
Beta-hemolytic Streptococcus
(S. pyogenes, S. agalactiae)
3. E. coli
4. Proteus
Klebsiella
Anaerobes
Pseudomonas
Acinetobacter .

13. Bacterial Infection of Wound Lab. Diagnosis
specimen collection.
Tissue biopsy
Needle aspirate
Wound swab
(Surface or Deep wound)

14. Bacterial Infection of Wound Lab. Diagnosis
Suspected organism
Bite wounds
Eikenella corrodens, Pasteurella multocida, Pasteurella canis, Staphylococcus aureus, group A Streptococcus, mixed anaerobe, many gram-negative bacilli.
Surgical wounds ­
Staphylococr:us aureus, coagulase-negative Staphylococcus, groups A and B Streptococci, Clostridium perfringens, Corynebacterium species many other bacteria.
Traumatic wounds
Bacillus species, Staphylococcus aureus, group A Strepiococcus, many gram­ negative bacilli.

15. Thank you
I would like to thank
Dr. Neal R. Chamberlain