1. Are The Drug Manufacturers Telling The Truth? Mary Kathryn Hardison

2. Objectives The purpose of our experiment was to find out if the label on the aspirin bottle telling how many milligrams were in one tablet were actually correct Why is this important? Drug companies are allowed not less than 90.0 percent and not more than 110.0 percent on the labled amount of aspirin

3. Objectives (cont) To study degradation of aspirin in water To see the effect of temperature on aspirin

4. Hydrolysis of Aspirin + Acetyl salicylic acid MW = 180.157 Salicylic acid 138.121 Acetic acid 60.052

5. What is an HPLC? HPLC uses high pressure to force mobile phase (MP) through closed column containing stationary phase (SP, fine particles) to give high-resolution separations MP – the solvent moving through the column SP – fine particles on the support that stay in place inside the column High Pressure/Performance Liquid Chromatography

8. What We Had to Do to Get Started First we had to make up our mobile phase Then we had to do our standard curves for our samples Now it is show time

10. SA Calibration Curve

11. Aspirin Calibration Curve

12. Show Time! To begin I had to weigh out each tablet Then I ground up 5 pills and weighed out the powder to the amount of one tablet Next I made up my solution and put the solution into vials then into the HPLC

13. Our Results

14. Our Results (cont)

15. What We Found BrandLabel mgAnal mg% Diff. BC Green74287418 GS Coated32541227 Bayer32537816 G.S32539823 Ecotrin500487-3 Anacin40049123

16. Why Is This Helpful to You? The results of this experiment are helpful to you because now you know which brand of aspirin really gives you the correct amount If the aspirin has more Salicylic acid than it is supposed to have the Aspirin will not meet the expiration date on the bottle

17. Summary Through this experience I have learned –how to operate an analytical instrument such as HPLC –preparation of standard solutions –how to analyze chemicals –Now I Know that the samples have close to what they are supposed to have –Water and temperature affect the degradation of aspirin

18. Any Questions??