1. InfraCal Ethanol Blend Analyzer Ethanol in Gasoline Calibration for Low Levels www.WilksIR.com

2. Calibration Standards Calibration Standards are available from Wilks Enterprise or you can make your own

3. Mix at least 5 standards within the expected measurement range Mix at least 5 standards within the expected measurement range A maximum of 20 calibration points can be entered in the calibration table A maximum of 20 calibration points can be entered in the calibration table Calibration Standards

4. Reference for Mixing Volumetric Standards % Gasoline Ethanol 519 ml1 ml 1018 ml2 ml 1517 ml3 ml 2016 ml4 ml 2515 ml5 ml 3014 ml6 ml 3513 ml7 ml 4012 ml8 ml 4511 ml9 ml 5010 ml10 ml % Gasoline Ethanol 559 ml11 ml 608 ml12 ml 657 ml13 ml 706 ml14 ml 755 ml15 ml 804 ml16 ml 853 ml17 ml 902 ml18 ml 951 ml19 ml 100020 ml

5. Zeroing on Gasoline: Background Measurement For the most accurate measurement, an ethanol free gasoline sample from the same stock as the ethanol/gas sample to be analyzed can be used to zero out any differences in gasoline makeup.

6. Since ethanol free gasoline is not always available, the ethanol can be extracted from the gasoline in order to zero the analyzer Zeroing on Gasoline: Background Measurement

7. To extract the ethanol, the gasoline sample is mixed with a salt water solution which collects the ethanol, leaving ethanol free gasoline Zeroing on Gasoline: Background Measurement

8. If ethanol free gasoline of the same type to be measured is available, this step can be skipped Note: If the gasolines to be analyzed are similar in chemical makeup, or if the measurement requirements allow up to a 1% error, zeroing can be done on a clean crystal without the base gasoline or the extraction step Zeroing on Gasoline: Background Measurement

9. Salt Water for Extraction 1.Add 1 liter of distilled water to container 2.Add 20g (1 tablespoon) of sodium chloride (table salt) to the water. The exact amount is not important. to the water. The exact amount is not important. 3. Stir the solution until all the salt is dissolved Note: This solution can be made in any quantity as long as the ratio of water to salt is kept similar Background Measurement

10. Ethanol Extraction: 1. Add 2 parts salt water and 1 part gasoline of sample to be analyzed to glass container (~ 10 ml of salt water to 5 ml gasoline) 2. Cover the container and shake for 30 seconds 3. Allow the solution to settle (~ 30 seconds) There should now be two layers in the container. The top layer is ethanol free gasoline 4. There should now be two layers in the container. The top layer is ethanol free gasoline

11. Clean the crystal with a delicate task wipe cleaning from one end of the crystal to the other Background Measurement

12. Using a pipette, carefully remove approximately 2 ml of zero sample from the top layer of the extracted gasoline. Do not allow the pipette to come in contact with the bottom layer. Background Measurement

13. Apply the sample so that the entire crystal is wet with sample and cover with the stainless steel cover Background Measurement

14. Press and hold the ZERO button until the display reads bAL Recall ZERO CAL RUN bAL Background Measurement

15. Recall ZERO CAL RUN 1.267 A multiplier value to 3 decimal places will be displayed when zero is established. The actual value is only of interest when reporting problems to the factory Background Measurement

16. Recall ZERO CAL RUN 0.0 Press RUN. The display should read 0.0 + 0.2 If not, repeat the zero process Background Measurement

17. Run each standard at least 3 times Average results for each standard Enter results in a calibration table in column “A” and the corresponding calibration value in column “C” (see next slide) Calibration with Prepared Standards

18. AAbsorbanceC Calibration Standard AO1= 4.2 CO1= 2.5 AO2= 8.1 CO2= 5.0 AO3= 14.9 AO3= 14.9 CO3= 10.0 CO3= 10.0 Example Calibration Table

19. Entering Values into EDIT program Make sure the Cal Lock switch on the back ofMake sure the Cal Lock switch on the back of the InfraCal is in the “I” position the InfraCal is in the “I” position

20. Press the CAL button for two seconds, until CALPress the CAL button for two seconds, until CAL appears on the display appears on the display Recall ZERO CAL RUN CAL Entering Values into EDIT program

21. Recall ZERO CAL RUN Edit Press RECALL until Edit is displayedPress RECALL until Edit is displayed Entering Values into EDIT program

22. Recall ZERO CAL RUN n= Press CAL. The display will read n= for a short timePress CAL. The display will read n= for a short time Entering Values into EDIT program

23. Recall ZERO CAL RUN 3 Set the number to the number of standards (for our example it will be 3) using the up and down arrowsSet the number to the number of standards (for our example it will be 3) using the up and down arrows Entering Values into EDIT program

24. Recall ZERO CAL RUN A01 Press CAL and the display will read A01= for a short timePress CAL and the display will read A01= for a short time Entering Values into EDIT program

25. Using the up and down arrows, scroll to the first absorbance value (for our example it will be 4.2)Using the up and down arrows, scroll to the first absorbance value (for our example it will be 4.2) Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 4.2 CO1= 2.5 AO2= 8.1 CO2= 5.0 AO3= 14.9 AO3= 14.9 CO3= 10.0 CO3= 10.0

26. Recall ZERO CAL RUN 4.2 Using the up and down arrows, scroll to the first absorbance value (for our example it will be 4.2)Using the up and down arrows, scroll to the first absorbance value (for our example it will be 4.2) Entering Values into EDIT program

27. Recall ZERO CAL RUN C01 Press CAL again and the display will read C01=Press CAL again and the display will read C01= Entering Values into EDIT program

28. Using the up and down arrows, scroll to the first calibration value (for our example it will be 2.5)Using the up and down arrows, scroll to the first calibration value (for our example it will be 2.5) Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 4.2 CO1= 2.5 AO2= 8.1 CO2= 5.0 AO3= 14.9 AO3= 14.9 CO3= 10.0 CO3= 10.0

29. Recall ZERO CAL RUN 2.5 Using the up and down arrows, scroll to the first absorbance value (for our example it will be 2.5)Using the up and down arrows, scroll to the first absorbance value (for our example it will be 2.5) Entering Values into EDIT program

30. Recall ZERO CAL RUN A02 Press CAL again and the display will read A02=Press CAL again and the display will read A02= Entering Values into EDIT program

31. Using the up and down arrows, scroll to the second absorbance value (for our example it will be 8.1)Using the up and down arrows, scroll to the second absorbance value (for our example it will be 8.1) Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 4.2 CO1= 2.5 AO2= 8.1 CO2= 5.0 AO3= 14.9 AO3= 14.9 CO3= 10.0 CO3= 10.0

32. Recall ZERO CAL RUN C02 Press CAL again and the display will read C02=Press CAL again and the display will read C02= Entering Values into EDIT program

33. Using the up and down arrows, scroll to the second calibration value (for our example it will be 5.0)Using the up and down arrows, scroll to the second calibration value (for our example it will be 5.0) Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 4.2 CO1= 2.5 AO2= 8.1 CO2= 5.0 AO3= 14.9 AO3= 14.9 CO3= 10.0 CO3= 10.0

34. Do the same for the remaining calibration valuesDo the same for the remaining calibration values Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 4.2 CO1= 2.5 AO2= 8.1 CO2= 5.0 AO3= 14.9 AO3= 14.9 CO3= 10.0 CO3= 10.0

35. Recall ZERO CAL RUN IDLE After the last value, the display will read IDLEAfter the last value, the display will read IDLE Entering Values into EDIT program The calibration is now stored and active in the InfraCal

36. InfraCal Ethanol Blend Analyzer Ethanol in Gasoline Calibration for Low Levels For questions please contact us at: tech@wilksIR.com, or 203-855-9136