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 -amanitin CHX 1 2 3 4 5 6 7 8 0 2 4 6 0 2 4 6 (h) K921 Ac-Abl Abl Additional file, Figure S4.

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Presentation on theme: " -amanitin CHX 1 2 3 4 5 6 7 8 0 2 4 6 0 2 4 6 (h) K921 Ac-Abl Abl Additional file, Figure S4."— Presentation transcript:

1  -amanitin CHX 1 2 3 4 5 6 7 8 0 2 4 6 0 2 4 6 (h) K921 Ac-Abl Abl Additional file, Figure S4

2 BAA+1 B ions+1 Y ions+2 B ions+2 Y ionsY 1 L114.17-57.59-14 2 K242.341317.58121.67659.3013 3 P339.451189.41170.23595.2112 4 A410.531092.29205.77546.6511 5 P507.651021.22254.33511.1110 6 P604.76924.10302.89462.559 7 P701.88826.98351.44414.008 8 P799.00729.87400.00365.447 9 P896.11632.75448.56316.886 10 A967.19535.64484.10268.325 11 C Me 1084.36464.56542.68232.784 12 T1185.46347.39593.24174.203 13 G1242.52246.28621.76123.652 14 K Ac -189.23-95.121 Suppl. Fig. 1B. MS/MS of the peptide ion at m/z 477.8, corresponding to the (M+3H)3+ species with a mass of 1431Da.

3 p300 p300 -/- HCT116 1 2 3 4 - + IR (5Gy) K921 Ac-Abl Abl Suppl. Fig. S2. p300 HAT is dispensable for IR-induced K921 acetylation. HCT116 and HCT116/p300 - cells wre either mock-treated or IR (5 Gy) exposed and harvested 4 h post-treatment. Lysates were adjusted for equal protein content and probed with K921 acetyl-Abl (top panel), anti-Abl antibody (middle panel) or anti-p300 antibody (bottom panel).

4  -amanitin CHX 1 2 3 4 5 6 7 8 0 2 4 6 0 2 4 6 (h) K921 Ac-Abl Abl Additional file 3, Figure S3


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