1. Probe set Gene SymbolGene TitleRefSeq Transcript IDp-value Fold- Change 226218_atIL7Rinterleukin 7 receptorNM_0021850.0159686.98753 206545_atCD28CD28 molecule NM_001243077 /// NM_001243078 /// NM_0061390.01659456.92511 210512_s_atVEGFAvascular endothelial growth factor A NM_001025366 /// NM_001025367 /// NM_001025368 /// NM_001025369 /// NM_0010253700.03008455.98444 210690_atKLRC4 killer cell lectin-like receptor subfamily C, member 4NM_0134310.04353955.81104 201694_s_atEGR1early growth response 1NM_0019640.006171365.16785 204798_atMYB v-myb myeloblastosis viral oncogene homolog (avian) NM_001130172 /// NM_001130173 /// NM_001161656 /// NM_001161657 /// NM_0011616580.000635864.94855 206546_atSYCP2synaptonemal complex protein 2NM_0142580.03203833.95423 208078_s_atSIK1salt-inducible kinase 1NM_1733540.03154493.74018 218829_s_atCHD7 chromodomain helicase DNA binding protein 7NM_0177800.001247223.41881 230170_atOSMoncostatin MNM_0205300.0381832.97723 212803_atNAB2 NGFI-A binding protein 2 (EGR1 binding protein 2)NM_0059670.0326752.79983 202431_s_atMYC v-myc myelocytomatosis viral oncogene homolog (avian)NM_0024670.009486682.7585 1555167_s_atNAMPT nicotinamide phosphoribosyltransferaseNM_005746 /// NM_1827900.009784632.6474 205479_s_atPLAUplasminogen activator, urokinaseNM_001145031 /// NM_0026580.002245632.63127 204562_atIRF4interferon regulatory factor 4 NM_001195286 /// NM_002460 /// NR_036585 /// NR_0460000.03599472.43914 229464_atMYEF2myelin expression factor 2NM_0161320.01853762.43343 1569149_atPDLIM7PDZ and LIM domain 7 (enigma) NM_005451 /// NM_203352 /// NM_203353 /// NM_2136360.01270952.29946 210229_s_atCSF2 colony stimulating factor 2 (granulocyte-macrophage)NM_0007580.04381072.29885 209921_atSLC7A11 solute carrier family 7 (anionic amino acid transporter light chain, xc- system)NM_0143310.02728862.29328 1560274_atWTAPWilms tumor 1 associated proteinNM_004906 /// NM_152857 /// NM_1528580.005070622.26454 203372_s_atSOCS2suppressor of cytokine signaling 2 NM_001270467 /// NM_001270468 /// NM_001270469 /// NM_001270470 /// NM_0012704710.03589222.21727 218918_atMAN1C1 mannosidase, alpha, class 1C, member 1NM_0203790.03922922.1999 217594_atZCCHC11 zinc finger, CCHC domain containing 11 NM_001009881 /// NM_001009882 /// NM_0152690.01377472.18895 219355_atCXorf57chromosome X open reading frame 57NM_001184782 /// NM_0180150.04016622.17188 220358_atBATF3 basic leucine zipper transcription factor, ATF-like 3NM_0186640.01102112.15717 232879_atCRTC3 CREB regulated transcription coactivator 3NM_001042574 /// NM_0227690.008678652.13572 203140_atBCL6B-cell CLL/lymphoma 6 NM_001130845 /// NM_001134738 /// NM_001706 /// NM_1389310.001282912.1305 213596_atCASP4 caspase 4, apoptosis-related cysteine peptidaseNM_001225 /// NM_033306 /// NM_0333070.01880632.12182 211139_s_atNAB1 NGFI-A binding protein 1 (EGR1 binding protein 1)NM_0059660.02956772.10901 239648_atDCUN1D3 DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae)NM_1734750.02016872.09991 232487_atSFT2D1SFT2 domain containing 1NM_1451690.04893792.09813 220843_s_atDCAF13DDB1 and CUL4 associated factor 13NM_0154200.03176072.0711 242260_atMATR3Matrin 3 NM_001194954 /// NM_001194955 /// NM_001194956 /// NM_018834 /// NM_199189 /// N0.01278172.06872 221569_atAHI1Abelson helper integration site 1 NM_001134830 /// NM_001134831 /// NM_001134832 /// NM_0176510.04245712.01295 205891_at ADORA2B /// LOC10065 2851 /// LOC10065 3318 adenosine A2b receptor /// uncharacterized LOC100652851 /// uncharacterized LOC1 NM_000676 /// XM_003403468 /// XM_0034036840.0434844-2.00623 209201_x_atCXCR4chemokine (C-X-C motif) receptor 4NM_001008540 /// NM_0034670.0307514-2.01292 Supplementary Table 1: Differentially expressed genes between persisting and non-persisting clones (page1)

2. Probe set Gene SymbolGene TitleRefSeq Transcript IDp-value Fold- Change 217542_atMDM2 Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) NM_001145336 /// NM_001145337 /// NM_001145339 /// NM_001145340 /// NM_002392 //0.02245-2.03109 225974_atTMEM64transmembrane protein 64NM_001008495 /// NM_0011462730.013722-2.0312 239118_atKCNA2 potassium voltage-gated channel, shaker-related subfamily, member 2NM_001204269 /// NM_0049740.0382185-2.05798 219439_atC1GALT1 core 1 synthase, glycoprotein-N- acetylgalactosamine 3-beta- galactosyltransferaseNM_0201560.0280939-2.06772 209784_s_atJAG2jagged 2NM_002226 /// NM_1451590.00311047-2.08176 205174_s_atQPCTglutaminyl-peptide cyclotransferaseNM_0124130.00719158-2.08608 235318_atFBN1fibrillin 1NM_0001380.0400623-2.10427 239208_s_atYBEYYbeY metallopeptidase (putative)NM_001006114 /// NM_0581810.0101511-2.11358 238935_atRPS27Lribosomal protein S27-likeNM_0159200.0146375-2.11699 229242_atTNFSF15 tumor necrosis factor (ligand) superfamily, member 15NM_001204344 /// NM_0051180.00274354-2.12396 204149_s_atGSTM4glutathione S-transferase mu 4 NM_000850 /// NM_147148 /// NM_147149 /// NR_0245380.00208468-2.14684 227030_atIKZF3IKAROS family zinc finger 3 (Aiolos) NM_001257408 /// NM_001257409 /// NM_001257410 /// NM_001257411 /// NM_0012574120.0115875-2.15546 219305_x_atFBXO2F-box protein 2NM_0121680.0410813-2.16626 223207_x_atPHPT1phosphohistidine phosphatase 1 NM_001135860 /// NM_001135861 /// NM_0141720.00035939-2.18144 230224_atZCCHC18 zinc finger, CCHC domain containing 18NM_001143978 /// NR_0266940.0382822-2.18652 202887_s_atDDIT4DNA-damage-inducible transcript 4NM_0190580.0227037-2.18986 227193_atSLC30A4 solute carrier family 30 (zinc transporter), member 4NM_0133090.00401091-2.2385 205692_s_atCD38CD38 moleculeNM_0017750.0204432-2.24379 209295_at TNFRSF10 B tumor necrosis factor receptor superfamily, member 10bNM_003842 /// NM_147187 /// NR_0271400.00054101-2.24849 211833_s_atBAXBCL2-associated X protein NM_004324 /// NM_138761 /// NM_138762 /// NM_138763 /// NM_138764 /// NR_0278820.00021409-2.27993 1556308_atPRRT3proline-rich transmembrane protein 3NM_2073510.0124444-2.29529 218560_s_atJMJD4jumonji domain containing 4NM_001161465 /// NM_0230070.00652396-2.35608 204613_atPLCG2 phospholipase C, gamma 2 (phosphatidylinositol-specific)NM_0026610.0109426-2.36139 209644_x_atCDKN2Acyclin-dependent kinase inhibitor 2A NM_000077 /// NM_001195132 /// NM_058195 /// NM_058196 /// NM_0581970.00840063-2.3787 1553311_atC20orf197 chromosome 20 open reading frame 197NM_1736440.0407092-2.38472 207813_s_atFDXRferredoxin reductase NM_001258012 /// NM_001258013 /// NM_001258014 /// NM_001258015 /// NM_0012580160.00294989-2.39201 207167_atCD101CD101 molecule NM_001256106 /// NM_001256109 /// NM_001256111 /// NM_0042580.0179875-2.39784 218634_atPHLDA3 pleckstrin homology-like domain, family A, member 3NM_0123960.00075392-2.40344 242961_x_atDDX58 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58NM_0143140.0251973-2.42169 202672_s_atATF3activating transcription factor 3 NM_001030287 /// NM_001040619 /// NM_001206484 /// NM_001206485 /// NM_0012064862.46E-02-2.42884 209366_x_atCYB5Acytochrome b5 type A (microsomal) NM_001190807 /// NM_001914 /// NM_1489230.044212-2.43949 244413_atCLECL1C-type lectin-like 1 NM_001253750 /// NM_001267701 /// NM_1720040.0449327-2.44188 225912_atTP53INP1 tumor protein p53 inducible nuclear protein 1NM_001135733 /// NM_0332850.0102333-2.46809 227522_atCMBL carboxymethylenebutenolidase homolog (Pseudomonas)NM_1388090.00698173-2.48743 200644_at MARCKSL 1MARCKS-like 1NM_023009 /// NR_0528520.0378812-2.5054 204584_atL1CAML1 cell adhesion molecule NM_000425 /// NM_001143963 /// NM_0240030.0431925-2.51809 224937_atPTGFRN prostaglandin F2 receptor negative regulatorNM_0204400.0246948-2.59062 Supplementary Table 1: Differentially expressed genes between persisting and non-persisting clones (page2)

3. Probe set Gene SymbolGene TitleRefSeq Transcript IDp-value Fold- Change 231732_atSMPD3 sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase IINM_0186670.00018914-2.6679 206090_s_at DISC1 /// TSNAX- DISC1 disrupted in schizophrenia 1 /// TSNAX- DISC1 readthrough NM_001012957 /// NM_001012958 /// NM_001012959 /// NM_001039383 /// NM_0011645370.0430998-2.70058 203725_atGADD45A growth arrest and DNA-damage- inducible, alpha NM_001199741 /// NM_001199742 /// NM_0019240.00080435-2.70983 204114_atNID2nidogen 2 (osteonidogen)NM_0073610.0238238-2.72711 210762_s_atDLC1deleted in liver cancer 1 NM_001164271 /// NM_006094 /// NM_024767 /// NM_1826430.00167513-2.77946 202644_s_atTNFAIP3 tumor necrosis factor, alpha-induced protein 3NM_0062900.0371965-2.86641 226137_atZFHX3zinc finger homeobox 3NM_001164766 /// NM_0068850.0182354-2.8687 206307_s_atFOXD1forkhead box D1NM_0044720.0261468-2.87306 204748_atPTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygeNM_0009630.0306956-2.93479 229254_atMFSD4 major facilitator superfamily domain containing 4NM_1816440.00137903-2.94215 210609_s_atTP53I3tumor protein p53 inducible protein 3 NM_001206802 /// NM_004881 /// NM_1471840.0255089-2.95456 209016_s_atKRT7keratin 7NM_0055560.0396377-3.07262 221538_s_atPLXNA1plexin A1NM_0322420.016441-3.07289 226668_atWDSUB1 WD repeat, sterile alpha motif and U- box domain containing 1 NM_001128212 /// NM_001128213 /// NM_1525280.0459202-3.13932 227221_atZMAT3zinc finger, matrin-type 3NM_022470 /// NM_1522400.00030794-3.14659 208923_atCYFIP1cytoplasmic FMR1 interacting protein 1NM_001033028 /// NM_0146080.00526256-3.215 225606_atBCL2L11BCL2-like 11 (apoptosis facilitator) NM_001204106 /// NM_001204107 /// NM_001204108 /// NM_001204109 /// NM_0012041100.0333319-3.25634 229971_atGPR114G protein-coupled receptor 114NM_1538370.0153434-3.2795 206907_atTNFSF9 tumor necrosis factor (ligand) superfamily, member 9NM_0038110.00179722-3.2986 206508_atCD70CD70 moleculeNM_0012520.00246867-3.33596 226225_atMCCmutated in colorectal cancersNM_001085377 /// NM_0023870.0209347-3.38257 207426_s_atTNFSF4 tumor necrosis factor (ligand) superfamily, member 4NM_0033260.00050034-3.83688 229797_atMCOLN3mucolipin 3NM_001253693 /// NM_0182980.0404844-3.8634 228937_atLACC1 laccase (multicopper oxidoreductase) domain containing 1NM_001128303 /// NM_1532180.00276434-3.96617 206873_atCA6carbonic anhydrase VINM_0012150.00508658-4.07431 208890_s_atPLXNB2plexin B2NM_0124010.0103517-4.4534 208131_s_atPTGIS prostaglandin I2 (prostacyclin) synthaseNM_0009610.0449316-4.49543 205569_atLAMP3 lysosomal-associated membrane protein 3NM_0143980.00381878-4.6834 204777_s_atMALmal, T-cell differentiation protein NM_002371 /// NM_022438 /// NM_022439 /// NM_0224400.0236599-4.79926 211144_x_at TARP /// TRGC2 TCR gamma alternate reading frame protein /// T cell receptor gamma constant 2NM_001003799 /// NM_0010038060.0497887-5.9034 201417_atSOX4SRY (sex determining region Y)-box 4NM_0031070.00129016-6.44274 213135_atTIAM1 T-cell lymphoma invasion and metastasis 1NM_0032530.0222017-6.71067 236313_atCDKN2B cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)NM_004936 /// NM_0784870.00837304-7.81711 Supplementary Table 1: Differentially expressed genes between persisting and non-persisting clones (page3)

4. Supplementary Figure 1 % IL-7R expressing cells in the infusion bag correlates with persistence of CD8+ effector clones. Correlation between pre-infusion % of IL-7R expressing cells in the infusion bag of CD8+ T effector clones (n=9) and peripheral blood persistence (% CD8+Tet+) at day 30 after adoptive transfer. % IL-7R cells Clonal persistence (% CD8+Tet+) R 2 = 0.71 P = 0.004

5. Supplementary Figure 2 Representative phenotypic profiling of IL7R+ and IL7R- subsets found within infused effector clones Pre-infusion phenotype of IL7R+ and IL-7R- subsets identified in infused M4 clone by flow cytometry. For reference, phenotyping of IL7R+ and IL-7R- subsets within open repertoire autologous PBL (CD3+CD8+ gated) is shown. Gray shaded histogram represents isotype staining. Staining of IL-7R+ cells are shown in red outline and IL-7R- cells are shown in blue outline. Numbers in plots are similarly color coded to denote % shift from isotype.

6. Supplementary Figure 3 IL-7R expression correlates with c-myc mRNA expression in CD8+ effector clones. Correlation between IL-7R cell surface expression (MFI) and c-myc mRNA (per 10 5  -actin copies) expression in infused CD8+ effector clones (n=6).

7. Supplementary Figure 4 IL-7R expression correlates with induced pSTAT5 expression in CD8+ effector clones. Correlation between IL-7R cell surface expression (MFI) and IL-7-induced pSTAT5 expression in infused CD8+ effector clones (n=7).