1. OXFAM-DELAGUA Water analysis kit

2. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 2 Contents 1.Components 2.Capabilities 3.Maintenance 4. Summary of bacteriological analysis process Título de la presentación

3. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 3 1. Componentes 1. Components

4. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 4

5. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 5

6. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 6 –Filtration apparatus –Incubator –Culture Medium –Petri Dishes –Sampling Methods –Turbidimeter –pH and Chlorine meters –Consumables

7. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 7 Filtration apparatus

8. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 8 Spares case

9. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 9 External battery

10. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 10 2. Capabilities

11. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 11 CAPABILITIES Turbidity pH Free and total chlorine Bacteriological analysis (thermotolerant faecal coliforms)

12. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 12 3. Maintenace

13. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 13 GENERAL MAINTENANCE Weekly Wash, rinse and dry the filtration apparatus Apply a smear of silicone grease to the black rubber O-ring Charge the internal battery fully at the end of each week CAREFULLY READ THE MANUAL Monthly Check the incubator temperature and recalibrate if necessary Check all the kit components

14. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 14 BATTERY Never allow the internal battery to discharge fully. Never leave the incubator switched on for more than 18 consecutive hours Always recharge the battery at the end of the working period in the field. Recharge monthly during storage. CAREFULLY READ THE MANUAL

15. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 15 4. Summary of the bacteriological analysis process

16. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 16 Preparation: –Sterilization of equipment: (autoclave, pressure cooker, methanol, boiling) –Petri: 15 min in autoclave or with methanol –Filtration apparatus: 15 min with methanol –Culture medium (38 gr/500 ml distilled water) –Petri dishes (Up to 16 daily samples) Filtration process Filtration volume (10 ml, 50 ml,100 ml) Filtration control Incubation (16-18 H 44ºC) Counting colonies: Yellow colour (faecal coliforms)

17. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 17 Presentation of results The results are presented as CFU/100ml CFU (Colony forming units) 100 ml of sample (treated or very clean water) have been filtered: CFU/100ml = nº of identified colonies as coliforms 50 ml of sample have been filtered: CFU/100ml = nº of identified colonies as coliforms x 2 10 ml of sample have been filtered: CFU/100ml = nº of identified colonies as coliforms x 10 It is possible to use smaller volumes in samples with higher contamination. The results should be calculated multiplying by the correspondent dilution factor.

18. OXFAM-DELAGUA water analysis kit WATSAN M15 ERU 18 Thank you……….