1. © 2011 Fluxion Biosciences. All rights reserved. Brief timeline and flunding history

2. Page 2 © 2011 Fluxion Biosciences. All rights reserved. Fluxion today BioFlux 200BioFlux 1000IonFlux-16IonFlux HT Company: –Venture backed since Dec 2007 –4 products commercialized and selling globally –Segment: Biotools –Over 100 units installed Products: instruments and consumables –BioFlux System for cell-cell interactions –IonFlux Automated Patch Clamp System –IsoFlux rare cell isolation platform – under development

3. Page 3 © 2011 Fluxion Biosciences. All rights reserved. Partial customer list Johnson & Johnson

4. Page 4 © 2011 Fluxion Biosciences. All rights reserved. IonFlux applications Compound profiling –Dose response measurements –Hit confirmation –Single concentration screening Mutant screening –Evaluate functional changes dependent on mutations –Record responses from up to 32 distinct mutants (8 for IF-HT) Structure-function studies –Determine the effect of mutations on channel function –Characterize expression for differentiated stem cells –Recording endogenously expressed ion channels –Screen clones based on ion channel density

5. Page 5 © 2011 Fluxion Biosciences. All rights reserved. IonFlux Technology for APC - Best in Class High quality data –G-seal quality recordings –Ensemble recording for improved success rate Cost effective –Perform APC for about the cost of an MPC setup Simple to use –Ease of use of a ‘plate reader’ –Relevance of patch clamp data –Table top unit Reliable –No robotics required –Only two moving parts

6. Page 6 © 2011 Fluxion Biosciences. All rights reserved. Ensemble vs. single cell recording Ensembles average signals over 20 patched cells (16 ensembles per plate, left panel) The new single cell consumable records from one cell per channel (16 cells per plate for IF-16, right panel) The single cell consumable utilizes the same hardware 20-cell ensemble Single cell

7. Page 7 © 2011 Fluxion Biosciences. All rights reserved.© 2010 Fluxion Biosciences. All rights reserved. Well-Plate Integrated Microfluidics Well-plate integrated microfluidics (WPM) utilizes standard 96- and 384- well plates, but the bottom of the plate is replaced with a microfluidic network interconnected between the wells.

8. Page 8 © 2011 Fluxion Biosciences. All rights reserved. * Sample customer slide – Pfizer UK

9. Page 9 © 2011 Fluxion Biosciences. All rights reserved. High Level Timeline Founded 200620072008200920102011 Grant 1 Ph2 Grant 1 Ph1 Grant 2 Ph2 Grant 2 Ph1 BioFlux product launch IonFlux product launch Series A Series B

10. Page 10 © 2011 Fluxion Biosciences. All rights reserved. Grant pipeline and success rates

11. Page 11 © 2011 Fluxion Biosciences. All rights reserved. SBIR: the good, the bad and the ugly The good: –Non-dilutive capital! –Can be the catalyst for new projects, encourages innovation –Early $$, which are the hardest The bad: –Long timelines: 9 mo. from application to funding –<200k is low capital for Phase I awards –Can be a distraction from the central mission The ugly: –Review process dependent on random events –In our case, projects with the greatest commercial worth didn’t get funded