1. www.wilksIR.com InfraCal Biodiesel Blend Analyzer Biodiesel in Diesel Calibration

2. Calibration Standards Calibration Standards are available from Wilks Enterprise

3. Calibration Standards Or you can make your own

4. Calibration Standards Biodiesel – choose the most common feedstock Diesel -- use the local diesel

5. Mix at least 5 standards within the expected measurement range. Mix at least 5 standards within the expected measurement range. A maximum of 20 calibration points can be entered in the calibration table A maximum of 20 calibration points can be entered in the calibration table Calibration Standards

6. Reference for Mixing Volumetric Standards % Diesel Biodiesel 519 ml1 ml 1018 ml2 ml 1517 ml3 ml 2016 ml4 ml 2515 ml5 ml 3014 ml6 ml 3513 ml7 ml 4012 ml8 ml 4511 ml9 ml 5010 ml10 ml % Diesel Biodiesel 559 ml11 ml 608 ml12 ml 657 ml13 ml 706 ml14 ml 755 ml15 ml 804 ml16 ml 853 ml17 ml 902 ml18 ml 951 ml19 ml 100020 ml

7. Allow InfraCal Analyzer to warm up Allow InfraCal Analyzer to warm up for at least 1 hour before calibration for at least 1 hour before calibration Make sure crystal on the sample Make sure crystal on the sample plate is clean plate is clean Calibration with Prepared Standards

8. Cleaning the Crystal on the Sample Plate Add enough hexane or appropriate solvent to cover crystal Wipe slowly across the crystal in one direction, wiping back and forth tends to spread residual fuel Repeat cleaning to insure previous fuel is off Repeat cleaning to insure previous fuel is off

9. Zeroing Press and hold the ZERO button until the display reads bAL. Press and hold the ZERO button until the display reads bAL. Recall ZERO CAL RUN bAL

10. A multiplier value to 3 decimal places will be displayed when zero is established. The actual value is only of interest when reporting problems to the factoryA multiplier value to 3 decimal places will be displayed when zero is established. The actual value is only of interest when reporting problems to the factory Recall ZERO CAL RUN 1.267 Zeroing

11. Press RUN. The display should read 0.0 + 2.0. If not, repeat the zero processPress RUN. The display should read 0.0 + 2.0. If not, repeat the zero process Recall ZERO CAL RUN 0.0 Zeroing Note: The result should be zero within +/- 0.2 after calibration and between +/-2.0 prior to calibration.

12. Run each standard at least 3 times starting with the zero standard (100% diesel)- cleaning between each sample Average results for each standard Enter results in a calibration table in column “A” and the corresponding calibration value in column “C” (see next slide) Calibration with Prepared Standards

13. Since the InfraCal does not allow a concentration of zero to be entered into the calibration table, the first concentration point must be calculated at 0.1%. See next slide for calculation…

14. To do this, use the following equation:A01=((A02-A00)/C02)*0.1+A00 A01 is the calculated relative absorbance value to enter into the instrument for C01= 0.1A01 is the calculated relative absorbance value to enter into the instrument for C01= 0.1 A00 is the relative absorbance of the 0% biodiesel (pure diesel) standardA00 is the relative absorbance of the 0% biodiesel (pure diesel) standard A02 is the relative absorbance of the second biodiesel standard (5% standard with standards from Wilks)A02 is the relative absorbance of the second biodiesel standard (5% standard with standards from Wilks) C02 is the concentration of the second biodiesel standard (5.0 with standards from Wilks)C02 is the concentration of the second biodiesel standard (5.0 with standards from Wilks)

15. Note: This calculation is done automatically in the Excel file that can be found on the CD included with the instrument. Contact: tech@WilksIR.com tech@WilksIR.com if you would like that file emailed to you

16. AAbsorbanceC Calibration Standard AO1= Calculated value CO1= 0.1 AO2= 4.2 CO2= 2.5 AO3= 8.1 CO3= 5.0 AO4= 14.9 AO4= 14.9 CO4= 10.0 CO4= 10.0 Example Calibration Table Note: Because a 0.0 value can’t be entered into the calibration, the value for C01 will be“0.1”

17. Entering Values into EDIT program Make sure the Cal Lock switch on the back ofMake sure the Cal Lock switch on the back of the InfraCal is in the “I” position the InfraCal is in the “I” position

18. Press the CAL button for two seconds, until CALPress the CAL button for two seconds, until CAL appears on the display appears on the display Recall ZERO CAL RUN CAL Entering Values into EDIT program

19. Recall ZERO CAL RUN Edit Press RECALL until Edit is displayedPress RECALL until Edit is displayed Entering Values into EDIT program

20. Recall ZERO CAL RUN n= Press CAL. The display will read n= for a short timePress CAL. The display will read n= for a short time Entering Values into EDIT program

21. Recall ZERO CAL RUN 4 Set the number to the number of standards (for our example it will be 4) using the up and down arrowsSet the number to the number of standards (for our example it will be 4) using the up and down arrows Entering Values into EDIT program

22. Recall ZERO CAL RUN A01 Press CAL and the display will read A01= for a short timePress CAL and the display will read A01= for a short time Entering Values into EDIT program

23. AAbsorbanceC Calibration Standard AO1= (0.3) Calculated value CO1= 0.1 AO2= 4.2 CO2= 2.5 AO3= 8.1 CO3= 5.0 AO4= 14.9 AO4= 14.9 CO4= 10.0 CO4= 10.0 Using the up and down arrows, scroll to the first absorbance value (for our example it will be 0.3)Using the up and down arrows, scroll to the first absorbance value (for our example it will be 0.3) Entering Values into EDIT program

24. Recall ZERO CAL RUN 0.3 Using the up and down arrows, scroll to the first absorbance value (for our example the calculated value will be 0.3)Using the up and down arrows, scroll to the first absorbance value (for our example the calculated value will be 0.3) Entering Values into EDIT program

25. Recall ZERO CAL RUN C01 Press CAL again and the display will read C01=Press CAL again and the display will read C01= Entering Values into EDIT program

26. AAbsorbanceC Calibration Standard AO1= 0.3 CO1= 0.1 AO2= 4.2 CO2= 2.5 AO3= 8.1 CO3= 5.0 AO4= 14.9 AO4= 14.9 CO4= 10.0 CO4= 10.0 Using the up and down arrows, scroll to the first calibration value (for our example it will be 0.1)Using the up and down arrows, scroll to the first calibration value (for our example it will be 0.1) Entering Values into EDIT program

27. Recall ZERO CAL RUN 0.1 Using the up and down arrows, scroll to the first absorbance value (for our example it will be 0.1)Using the up and down arrows, scroll to the first absorbance value (for our example it will be 0.1) Entering Values into EDIT program

28. Recall ZERO CAL RUN A02 Press CAL again and the display will read A02=Press CAL again and the display will read A02= Entering Values into EDIT program

29. AAbsorbanceC Calibration Standard AO1= 0.3 CO1= 0.0 AO2= 4.2 CO2= 2.5 AO3= 8.1 CO3= 5.0 AO4= 14.9 AO4= 14.9 CO4= 10.0 CO4= 10.0 Using the up and down arrows, scroll to the second absorbance value (for our example it will be 4.2)Using the up and down arrows, scroll to the second absorbance value (for our example it will be 4.2) Entering Values into EDIT program

30. Recall ZERO CAL RUN C02 Press CAL again and the display will read C02=Press CAL again and the display will read C02= Entering Values into EDIT program

31. AAbsorbanceC Calibration Standard AO1= 0.3 CO1= 0.0 AO2= 4.2 CO2= 2.5 AO3= 8.1 CO3= 5.0 AO4= 14.9 AO4= 14.9 CO4= 10.0 CO4= 10.0 Using the up and down arrows, scroll to the second calibration value (for our example it will be 2.5)Using the up and down arrows, scroll to the second calibration value (for our example it will be 2.5) Entering Values into EDIT program

32. AAbsorbanceC Calibration Standard AO1= 0.3 CO1= 0.0 AO2= 4.2 CO2= 2.5 AO3= 8.1 CO3= 5.0 AO4= 14.9 AO4= 14.9 CO4= 10.0 CO4= 10.0 Do the same for the remaining calibration valuesDo the same for the remaining calibration values Entering Values into EDIT program

33. Recall ZERO CAL RUN IDLE After the last value, the display will read IDLEAfter the last value, the display will read IDLE Entering Values into EDIT program The calibration is now stored and active in the InfraCal

34. First insure sample plate is clean Add enough hexane or appropriate solvent to cover crystal Wipe slowly across the crystal in one direction, wiping back and forth tends to spread residual fuel Repeat cleaning to insure previous fuel is off Repeat cleaning to insure previous fuel is off Sample Analysis

35. Apply approximately 2ml of the fuel sample to Apply approximately 2ml of the fuel sample to the crystal ensuring that the entire crystal is the crystal ensuring that the entire crystal is covered covered

36. Recall ZERO CAL RUN 0.0 Sample Analysis Press RUNPress RUN

37. Recall ZERO CAL RUN 5.7 Sample Analysis After 15 seconds the result is displayed After 15 seconds the result is displayed

38. For questions please contact us at: tech@wilksIR.com, or 203-855-9136 InfraCal Biodiesel Blend Analyzer Biodiesel in Diesel Calibration