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William S. Klug Michael R. Cummings Charlotte A. Spencer Concepts of Genetics Eighth Edition Chapter 16 Regulation of Gene Expression in Prokaryotes Copyright © 2006 Pearson Prentice Hall, Inc.
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Prokaryotes Exhibit Efficient Genetic Mechanisms to Respond to Environmental Conditions
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Lactose Metabolism in E. coli Is Regulated by an Inducible System
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Figure 16-1 Copyright © 2006 Pearson Prentice Hall, Inc.
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Structural Genes
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Figure 16-2 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-3 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-4 Copyright © 2006 Pearson Prentice Hall, Inc. IPTG
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The Discovery of Regulatory Mutations (constitutive mutants) lacI - lacO C
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The Operon Model: Negative Control
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Figure 16-5 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-5a Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-5b Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-5c Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-6 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-6a Copyright © 2006 Pearson Prentice Hall, Inc. Repressor = trans control element
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Figure 16-6b Copyright © 2006 Pearson Prentice Hall, Inc. Operator = cis control element
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Genetic Proof of the Operon Model
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Table 16-1 Copyright © 2006 Pearson Prentice Hall, Inc. constitutive trans element cis element
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Figure 16-7 Copyright © 2006 Pearson Prentice Hall, Inc.
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The Catabolite-Activating Protein (CAP) Exerts Positive Control over the lac Operon Catabolite repression
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Figure 16-8 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-8a Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-8b Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-9 Copyright © 2006 Pearson Prentice Hall, Inc.
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Crystal Structure Analysis of Repressor Complexes Has Confirmed the Operon Model
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Figure 16-10 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-11 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-11a Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-111b Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-11c Copyright © 2006 Pearson Prentice Hall, Inc.
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The Tryptophan (trp) Operon in E. coli Is a Repressible Gene System
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Figure 16-12 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-12a Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-12b Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-12c Copyright © 2006 Pearson Prentice Hall, Inc.
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Attenuation Is a Critical Process during the Regulation of the trp Operon in E. coli
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Figure 16-13 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-13a Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-13b Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-13c Copyright © 2006 Pearson Prentice Hall, Inc.
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TRAP and AT Proteins Govern Attenuation in B. subtilis TRAP= trp RNA-binding attenuation protein AT= anti-TRAP
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Figure 16-14 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-14a Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-14b Copyright © 2006 Pearson Prentice Hall, Inc.
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The ara Operon Is Controlled by a Regulator Protein That Exerts Both Positive and Negative Control
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Figure 16-15 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-15a Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-15b Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 16-15c Copyright © 2006 Pearson Prentice Hall, Inc.
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