1. Manganese Oxidizing Bacteria Cynthia McClain HMC 2012 July 19, 2012

2. Mn(II) Mn(III,IV) Soluble Mn-oxide/oxyhydroxide precipitate Most MnOx in nature formed biotically!

3. Niche Physical and chemical gradients

4. Manganese Oxidizing Bacteria Multicopper oxidase or peroxidase proteins

5. Isolation 1.Collect water or sediment sample (slurry) 2.Streak 100-200µl supernatant on K media plate: MnCl 2, peptone, yeast extract 3.Incubate 1-3 weeks 4.Restreak brown colonies 5.Test with Leucoberbelin Blue (LBB)

6. Experimental Results Different morphologies – Color – Size Positive LBB – Soil – Sand from mouth of Elkhorn Slough (2) – Kirby Park oily sheen (1) – Baby seal beach sand (1)

7. Movie of LBB Test Sand from mouth of Elkhorn Slough

8. Soil

10. Kirby Park Oily Sheen

12. Baby Seal Beach

15. Manganese Oxidizing Bacteria Phylogeny Why oxidize Mn? Environmental Significance

16. Thank you!