Presentation on theme: "Purple Non Sulfur Bacteria"— Presentation transcript:
1 Purple Non Sulfur Bacteria Hopkins Microbiology CourseAnn Lesnefsky
2 Metabolism of PNS Bacteria Organic acid electron donorPhotosynthesis for energy generationBacterial chlorophyll bAbsorbs light in the infrared rangeCurrently PNS bacteria are being studied for H2 production
3 Course Enrichment Method for PNS Plated samples from the slough on succinate and ethanol plates, incubated anaerobically with lightCourse’s media and Holly’s mediaRestreak single colonies for isolation
4 TA Enrichment Method for PNS TA’s enriched for PNS by filteringFilter both water above mat and slurry of mat materialPlace filter face down on plateFlip and all allow PNS bacteria to grow on filter
5 Many Some Few Carbon Source Enrichment Method Succinate Ethanol (# of colonies before restreak)EthanolNo filterCourse436HollyFilterManySomeFewNone
6 Microscopy of PNS Colonies isolated from 5 different plates 4 plates from strawberry1 from Kerby ParkAll looked like stubby rods
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