Presentation on theme: "Purple Non Sulfur Bacteria Hopkins Microbiology Course Ann Lesnefsky 7-15-2011."— Presentation transcript:
Purple Non Sulfur Bacteria Hopkins Microbiology Course Ann Lesnefsky
Metabolism of PNS Bacteria Organic acid electron donor Photosynthesis for energy generation – Bacterial chlorophyll b – Absorbs light in the infrared range Currently PNS bacteria are being studied for H 2 production
Course Enrichment Method for PNS – Plated samples from the slough on succinate and ethanol plates, incubated anaerobically with light – Course’s media and Holly’s media – Restreak single colonies for isolation
TA Enrichment Method for PNS TA’s enriched for PNS by filtering – Filter both water above mat and slurry of mat material – Place filter face down on plate – Flip and all allow PNS bacteria to grow on filter
Carbon Source Enrichment Method Succinate (# of colonies before restreak) Ethanol (# of colonies before restreak) No filterCourse 43 6 Holly 0 0 FilterCourse Many Some Holly Few None
Microscopy of PNS Colonies isolated from 5 different plates – 4 plates from strawberry – 1 from Kerby Park All looked like stubby rods
Recommendations Next year use the filter method.