1. Details of… Endoparasites Laboratory Procedures

2. Remember Endoparasites?

3. Major Classes of Endoparasites Helminths  Nematodes  Tapeworms  Flukes Arthropods  Insects  Arachnids Protozoa

4. Nematodes In general are round in cross section, longer than they are wide and are unsegmented. Can be in a variety of lengths. (Males smaller than Females) Significant cause of disease in dogs and cats Have varied life cycles

5. Roundworms Medical Name: Toxocara canis, Toxocara cati, Toxascaris leonina All three of these are common among dogs and cats. Live in the small intestine Vary in length from 3 – 18 cm (average ~ 7” in length)

6. Roundworms – cont’d Methods of transmission:  Direct (fecal/oral)  Transplacental  Transmammary  Predation of foreign hosts

7. Roundworms – cont’d The eggs of T. canis can survive on the soil for several years. (Fresh feces are NOT infective) Eggs are ingested and hatch in stomach, penetrate bowel wall, enter portal bloodstream, wander through the liver and end up in the lungs. They are coughed up and swallowed, molt in stomach and mature in small intestine. Entire life cycle can take as little as 4-5 weeks.

8. Toxocara canis ova

9. Adult Roundworms

10. Toxocara sp. Clinical Signs Heavy infections can produce abdominal cramps and distention, and obstruction of the intestinal tract. Pulmonary edema, coughing, nasal discharge, and increased respiratory rates may also be seen. Vomiting, diarrhea, emaciation, dull hair coat and restlessness are also often seen.

11. Toxocara Transmission to Humans Toxocara larvae can cause:  Visceral larval migrans  Ocular larval migrans  Neural larval migrans

12. Hookworms Medical name: Ancylostoma caninum, Ancylostoma tubaeforme, Ancylostoma braziliense, and Uncinaria stenocephala  Of these, the most commonly seen is Ancylostoma caninum

13. A. caninum Seen in over 20% of adult dogs Live in the small intestine Methods of transmission:  Skin penetration  Direct (Fecal/Oral)  Transmammary/intrauterine

14. A. caninum Growth and maturation process after ingestion is between 18-21 days. Female can lay up to 30,000 eggs per day In environment, there are 3 stages: egg, hatchling and larvae. (Takes 5 days) Larval stage is infective stage.

15. A. Caninum - Migration Once in new host, some will burrow back into intestine to complete maturity Others may burrow from intestines to lungs and mature there.  Ascend up trachea, cause coughing and is swallowed.  Worm goes back to intestine to mature.

16. Ancylostoma ova

17. Adult Ancylostoma caninum

18. Ancylostoma Clinical Signs Major CS are associated with blood loss and GI irritation. Weakness, anemia, diarrhea, bloody or tarry stools, anorexia, depression, and death may also occur. Larvae may wander into other organs causing secondary signs of hepatitis and pneumonia

19. Ancylostoma Transmission to Humans Infective larvae can penetrate the skin causing cutaneous larval migrans

20. Whipworms Medical name:  Trichuris vulpis  Trichuris serrata  Trichuris campanula Of these, most common is Trichuris vulpis

21. T. vulpis Common in young and adult dogs (not often seen in very young b/c of prepatent period of 70- 190 days) Rare in cats Not often seen in very young animals Live in jejunum Method of transmission:  Primarily fecal/oral (direct)

22. T. vulpis Eggs are passed in the stool Eggs are ingested by host and hatch in small intestine. Larvae migrate caudally, where they mature.

23. T. vulpis – Clinical Signs Depend on the number of parasites present, but can include:  Intermittent diarrhea, weight loss, emaciation, anemia, abdominal pain, flatulence, and “flank suckling”.

24. Trichuris vulpis ova

25. Trichuris vulpis Eggs are double operculated  (Have 2 opercules, have an opercule on each end)  Makes it easier for larva to hatch from egg Eggs can live under proper conditions in the soil for up to five years.

26. Adult Whipworm

27. Heartworms Medical name: Dirofilaria immitis Vector is mosquito Common in dogs, cats, ferrets and some humans Pseudoheartworm exists that is not pathogenic (Dipetalonema reconditum)

28. Microfilaria v- Dipetalonema Remember that Microfilaria are one of the juvenile forms of Heartworm Dipetalonema are usually non-pathogenic There are 3 main physical differences between the two:  Head  Tail shape  Type of movement

29. Microfilaria v- Dipetalonema Microfilaria:  Straight tail  Tapered head  Undulating movement (no forward mvmt.) Dipetalonema:  Button-hooked tail  Blunt head  Definite forward movement

30. Dirofilaria immitis Named “Heartworm” because it lodges in the venous return of the heart; primarily the right atria, & right ventricle. Inhibits the flow of blood to and from the heart. Vector is mosquito Prepatent period = 6 months Adults can live up to ~3-5 years Microfilaria can live up to 3 years

32. D. Immitis – Clinical Signs Usually progressive as disease progresses.  Gradual weight loss  Loss of endurance  Coughing  Accumulation of abdominal fluid  Labored breathing  Increased temperature  Cyanotic MM

33. D. Immitis - Diagnosis “SNAP” test  Detects the hormone from the adult female worm Blood smear  Looks for microfilaria in the blood Once diagnosed, disease state is classified (graded) from 1-4

34. Classes of Heartworm Disease Class 1 (Mild)  Occasional cough, fatigue with exercise, or mild loss of condition Class 2 (Moderate)  Occasional cough, fatigue with exercise, or mild to moderate loss of condition Class 3 (Severe)  General loss of condition: fatigue with mild activity; occasional or persistent cough Class 4 (Very Severe) Caval Syndrome http://www.youtube.com/watch?v=VOLzFsNOJ- 4&feature=player_embedded http://www.youtube.com/watch?v=VOLzFsNOJ- 4&feature=player_embedded 

37. “Reverse D” of Caval Syndrome

38. Cestodes Dipylidium caninum (flea tapeworm) Taenia (tapeworm)

39. Dipylidium caninum (Flea tapeworm) Most common tapeworm found in dogs and cats Animal becomes infected by ingesting the intermediate host, the flea. Can infect humans Indicates presence of fleas May see on perianal area or can see egg baskets on fecal flotation. The asexual process of forming segments is termed strobilation

44. Taenia (Tapeworm) Found as taenia pisiformis, taenia hydatigena, and taenia ovis. Acquired by eating cysticerci (larval form) in rabbits. (Predation of the host)

45. Trematodes (Flukes) Platynosomum fastosum (lizard poisoning fluke of cats) Nanopyetus salmincola (salmon poisoning fluke of dogs) Alaria (intestinal flukes of dogs and cats) Fasciola Hepatica (common liver fluke)

46. Platynosomum fastosum (Lizard poisoning fluke of cats) Adult flukes of cats inhabit the liver, gall bladder, bile ducts, and small intestine.

47. Nanophyetus salmincola (Salmon poisoning fluke) Vector for Neorickettsia hemintheca, Salmon poisoning disease in dogs. Called “Elokomin fluke fever” Causes hemorrhagic enteritis with generalized lymphadenopathy, vomiting, diarrhea, lethargy, inappetence, fever, and death. Found in Pacific Northwest region of North America

48. Alaria (intestinal flukes) Found throughout the northern half of North America Mostly non-pathogenic. Lung migration may cause some damage Minor zoonotic potential from larval stages from intermediate hosts.

50. Fasciola Hepatica (Liver Fluke) Requires an ________________ as an intermediate host. Has been identified World-wide and is responsible for substantial losses of cattle and sheep. _____________ stage that migrates to the _________ after ingestion is the infective stage. Feeds on liver tissue for _________ before migrating to the bile duct where it begins to produce eggs. (Can reproduce ___________) Mild infection = about ________ eggs produced per day per fluke with one animal depositing about ______________ eggs daily in a pasture. Is capable of causing disease in humans who _______________________________ growing near snails habitat. Clinical signs in animals include ________________, and __________________. (_____________is only occasionally seen)

52. Fasciola Hepatica Ova

53. Coccidians and Protozoans Are single-celled organisims Are generally very host specific Primarily live in small intestine of dogs and cats. Rarely a problem in the mature animal.

54. Coccidians (Are a type of protozoa) Isospora spp. Toxoplasma gondii Cryptosporidium Sarcocystis

55. Coccidians – cont’d Produce a condition called “coccidiosis” in the young animal. Most common Clinical Sign is diarrhea which can lead to death, possibly due to dehydration and other loss of nourishment. Diagnosed by seeing oocyst in fecal floatation of fresh feces.

56. Eimeria oocyst vs- Nematode ova

57. Toxoplasma Is zoonotic Definitive host is any member of feline family Can cause placental barrier in humans and cause spontaneous abortion.

58. Sporozoites: “Super egg” that can remain dormant for a considerable amount of time in the environment until ingested by a host. Tachyzoites: “fast dividers” Are the eggs that are rapidly dividing and causing infection in the host. Bradyzoites: “slow dividers” Are the eggs that are semi-dormant in a host that is not the definitive host. Can begin life-cycle again if this host is consumed by definitive host. May also cause chronic infection.

59. Toxoplasma gondii (Toxo) May cause transient diarrhea in cats Highly pathogenic to humans, especially fetus in utero

61. Protozoans (Are not necessarily coccidians) Anaplasma (and other blood parasites) Giardia  Are a flagellate protozoan NOT a coccidian  Cause diarrhea  Occur as cysts or trophozoites in fecal floatation  Transmitted primarily by drinking stagnant water

62. Giardia trophozoite