1. Histology Slides for the Cardiovascular System Slides are presented in order of magnification As you view the following slides, make sure you can accomplish these goals: 1.Can you identify the vessels and tissue layers observable on the slides? 2.Can you identify the specific structures or layers indicated by the numbered arrows or brackets? At the end of a sequence, you will find the answers to the above for each vessel and layer

2. Overview (40X), Masson stain for connective tissue 4 4 2 1 3 3

3. 1 6 3 3 2 4 5 7 8 9 10 Overview (100X), Masson stain for connective tissue

4. Vein or Artery? 400X 9 10 11 8 12 13

5. 5 5 14 7 6 13 Vein or Artery? 400X

6. Artery, Vein, Nerve Tissue stained with Masson stain Slides 2 through 5: 1.Artery 2.Vein 3.Nerve fascicles 4.Skeletal Muscle Tissue 5.Lumen of the vein (with RBC’s) 6.Vein Tunica Media- smooth muscle layer 7.Vein Tunica Externa- connective tissue layer 8.Lumen of the Artery (with RBC’s) 9.Artery Tunica Externa- connective tissue layer 10.Artery Tunica Media- smooth muscle layer

7. Artery, Vein, Nerve Tissue stained with Masson stain Slides 2 through 5: 11. Artery Tunica Interna- endothelial cells (simple squamous) 12.Artery Tunica Interna- Internal elastic lamina 13.Capillary Tunica Interna- endothelial cells 14.Vein Tunica Interna- endothelial cells (simple squamous)

8. 1 2 3 2 3 2 4 Overview (40X), stained for elastic fibers

9. 8 15 12 2 10 9 16 Vein or Artery? 400X

10. 15 9 10 12 8

11. 6 7 3 1 16 5 Vein or Artery? 400X

12. 14 6 7 5 16 Vein or Artery? 400X

13. Artery, Vein, Nerve Tissue stained for Elastic Fibers Slides 8 through 12: 1.Artery 2.Vein 3.Nerve fascicles 4.Skeletal Muscle Tissue 5.Lumen of the vein 6.Vein Tunica Media- smooth muscle layer 7.Vein Tunica Externa- connective tissue layer 8.Lumen of the Artery (with RBC’s) 9.Artery Tunica Externa- connective tissue layer (demonstrating elastic fibers) 10. Artery Tunica Media- smooth muscle layer

14. Artery, Vein, Nerve Tissue stained for Elastic Fibers Slides 8 through 12: 11. Artery Tunica Interna- endothelial cells (simple squamous) 12.Artery Tunica Interna- internal elastic lamina 13.Capillary Tunica Interna- endothelial cells 14.Vein Tunica Interna- endothelial cells (dark band is due to the elastic fibers in the basement membrane) 15.Artery Tunica Media- external elastic lamina 16.Adipose cells

15. 16 5 Vein or Artery? 400X 8 14 7 6

16. 9 16 8 10 Vein or Artery? 100X 3 3 12 2

17. Vein or Artery? 400X 8 10 9 11 12

18. Artery, Vein, Nerve Tissue, Nonspecific Stain Slides 15 through 16: 1.Artery 2.Vein 3.Nerve fascicles 4.Skeletal Muscle Tissue 5.Lumen of the vein 6.Vein Tunica Media- smooth muscle layer 7.Vein Tunica Externa- connective tissue layer 8.Lumen of the Artery (with RBC’s) 9.Artery Tunica Externa- connective tissue layer (demonstrating elastic fibers) 10. Artery Tunica Media- smooth muscle layer

19. Artery, Vein, Nerve Tissue, Nonspecific Stain Slides 15 through 16: 11. Artery Tunica Interna- endothelial cells (simple squamous) 12.Artery Tunica Interna- internal elastic lamina 13.Capillary Tunica Interna- endothelial cells 14.Vein Tunica Interna- endothelial cells (simple squamous) 15.Artery Tunica Media- external elastic lamina 16.Adipose cells

20. Specific Kind of Tissue? 400X 1 2 3 2 2 4

21. Cardiac Muscle Tissue Slides 20: 1.Nucleus of a Cardiac Muscle Cell 2.Intercalated Disc 3.Branching Fibers 4.Capillary Tunica Interna- endothelial cells

22. Identify Blood cells 3 2 2 1

23. 3 4

24. 3 2 4

25. 3 3 3 6

26. 5 3 5 7

27. 2 3 3 3 7

28. Blood Cells Slides 22-27 1.Basophil 2.Small Lymphocyte 3.Neutrophil 4.Eosinophil 5.Monoctye 6.Large Lymphocyte 7.Platelets