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Methedology SCN Resistant Soy inoculated with Rhizobium, C.elegans and Paecilomyces lilacinus SCN Resistant Soy inoculated with Rhizobium and C.elegans.

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Presentation on theme: "Methedology SCN Resistant Soy inoculated with Rhizobium, C.elegans and Paecilomyces lilacinus SCN Resistant Soy inoculated with Rhizobium and C.elegans."— Presentation transcript:

1 Methedology SCN Resistant Soy inoculated with Rhizobium, C.elegans and Paecilomyces lilacinus SCN Resistant Soy inoculated with Rhizobium and C.elegans SCN Resistant Soy inoculated with Resistant Rhizobium, C.elegans and copper sulfate SCN Resistant Soy inoculated with Rhizobium, C.elegans and Chitin SCN Resistant Soy inoculated with Rhizobium, C.elegans and Clove Oil SCN Resistant Soy inoculated with Rhizobium, C.elegans and Pasteria Penetrans キ Plant HeightThe soy will be grown in individual pots with a sand soil mixture and the varidables will be measured: キ Nodule number キ C.elegan count キ Nitrogen Content キ Leaf Number キ Leaf Quality X5 The mean will be calculated + standard deviation and the data will be analysed with and ANOVA and Sheffe post hoc test SCN Resistant Soy, Chitin, Pasteuria Penetrans, Rhizobium, and C.Elegans SCN Resistant Soy, Clove Oil, Chitin, Rhizobium, and C.Elegans Rhizobium Leguminosarum Culture Rhizobium in petri dishes with agar that has a concentration of (1,2,3,4mM CuSo 4 ) and collect surviving rhizobium Collect surviving Rhizobium and culture them at a higher concentration. This graph shows the evolution of a resistant strain of Rhizobium. The x axis shows trial while the y axis shows resistance to Copper Sulfate (Cuso4). The resistance increased after each trial and rose to 30 millimoles. This graph shows average nitrogen plant and soil content. The x axis shows group while the y axis shows content. Groups 1 and 5 were the lowest, the other groups were similar. A statistical analysis by an ANOVA was taken (p<.05), followed by a Sheffe post hoc test. (n=400) Paecilomyces lilacinus ControlClove Oil Chitin Pasteuria Penetrans Resistant Rhizobium and CUSO 4 Chitin and Pasteuria Penetrans Chitin and Clove Oil N=400 This graph shows average nodule number in each pot. The x axis shows groups while the y axis shows nodule number. Group 7,8,4 had the most and group 5 had the least. A statistical analysis by an ANOVA was taken (p<.05), followed by a Sheffe post hoc test. (n=400) Paecilomyces lilacinus ControlClove Oil Chitin Pasteuria Penetrans Resistant Rhizobium and CUSO 4 Chitin and Pasteuria Penetrans Chitin and Clove Oil N=400 This graph shows the average number of leaves per plant in each group. The x axis shows the groups while the y axis shows the amount of leaves. Group 4 had the most leaves and group 5 had the least. A statistical analysis by an ANOVA was taken (p<.05), followed by a Sheffe post hoc test. n=400 Paecilomyces lilacinus ControlClove Oil Chitin Pasteuria Penetrans Resistant Rhizobium and CUSO 4 Chitin and Pasteuria Penetrans Chitin and Clove Oil N=400 This graph shows the average amount of C.Elegans in each plant. The x axis shows groups while the y axis shows C.Elegans. Group 2,3 had the most and Group 5,7 had the least. A statistical analysis by an ANOVA was taken (p<.05), followed by a Sheffe post hoc test. (n=400) Paecilomyces lilacinus ControlClove Oil Chitin Pasteuria Penetrans Resistant Rhizobium and CUSO 4 Chitin and Pasteuria Penetrans Chitin and Clove Oil N=400 This graph shows the average percentage of healthy leaves per plant. The x axis show the groups while the y axis shows the percentage. Group 7 had the most healthy leaves and group 5 had the most unhealthy leaves, while other groups were similar. A statistical analysis by an ANOVA was taken (p<.05), followed by a Sheffe post hoc test. (n=400) Paecilomyces lilacinus ControlClove Oil Chitin Pasteuria Penetrans Resistant Rhizobium and CUSO 4 Chitin and Pasteuria Penetrans Chitin and Clove Oil N=400 Paecilomyces lilacinus ControlClove Oil Chitin Pasteuria Penetrans Resistant Rhizobium and CUSO 4 Chitin and Pasteuria Penetrans Chitin and Clove Oil This graph shows the average height of each plant. The x axis shows the groups while the y axis shows the height in cm. The plants in group 7 were the tallest and in group 1 and 5 were the shortest. A statistical analysis by an ANOVA was taken (p<.05), followed by a Sheffe post hoc test. (n=400) N=400 This graph shows the average size of the nodules. The x axis shows group and the y axis shows nodule size in mm. Group 4,7 had the largest nodules and group 1,5 had the smallest nodules. A statistical analysis by an ANOVA was taken (p<.05), followed by a Sheffe post hoc test. (n=400) Paecilomyces lilacinus ControlClove Oil Chitin Pasteuria Penetrans Resistant Rhizobium and CUSO 4 Chitin and Pasteuria Penetrans Chitin and Clove Oil N=400 Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8


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