Presentation is loading. Please wait.

Presentation is loading. Please wait.

Fig. S1 siControl E2 G1: 45.7% S: 26.9% G2-M: 27.4% siER  E2 G1: 70.9% S: 9.9% G2-M: 19.2% G1: 57.1% S: 12.0% G2-M: 30.9% siRNF31 E2 A B siRNF31 siControl.

Published byHoward Wiggins Modified over 8 years ago

Similar presentations

Presentation on theme: "Fig. S1 siControl E2 G1: 45.7% S: 26.9% G2-M: 27.4% siER  E2 G1: 70.9% S: 9.9% G2-M: 19.2% G1: 57.1% S: 12.0% G2-M: 30.9% siRNF31 E2 A B siRNF31 siControl."— Presentation transcript:

1 Fig. S1 siControl E2 G1: 45.7% S: 26.9% G2-M: 27.4% siER  E2 G1: 70.9% S: 9.9% G2-M: 19.2% G1: 57.1% S: 12.0% G2-M: 30.9% siRNF31 E2 A B siRNF31 siControl siRNF31+ ERα plasmid G1: 57.6% S: 22.6% G2-M: 20.3% G1: 61.7% S: 13.8% G2-M: 24.9% G1: 55.4% S: 18.9% G2-M: 26.2%

2 Fig. S2 siControl siRNF31 T47D RNF31 ER  GAPDH RNF31 ER  GAPDH siControl siRNF31 MDAMB175 120 kDa 66 kDa 37 kDa 120 kDa 66 kDa 37 kDa 120 kDa 66 kDa 37 kDa siControl siRNF31 SMARTpool siRNF31 oligo 1 RNF31 ER  GAPDH A B siRNF31 oligo 2 1.2 1.0 0.8 0.6 0.4 0.2 0 Relative mRNA level RNF31 (mRNA)

3 Fig. S3 AB DC NA *** Relative luciferase activity NA Relative luciferase activity Relative mRNA level NA Relative mRNA level ***

4 Input IP: RNF31 IB: ER  IP IgG IP RNF31 Input IP IgG IP ER  IP: ER  IB: RNF31 Fig. S4 A B Input IgG IP RNF31 ER  IgG Input IgG IP RNF31 Ig G ER  Ig G Input IgG IP RNF31 C Input IgG IP ERα RNF31 Ig G RNF31 Ig G RNF31

5 Fig. S5 nucleus cytosol

6 Fig. S6 RNF31 ER  Merge Merge with nuclear stain

7 Fig. S7 Fold enrichment over IgG control 

8 Gene nameSequence RNF31 ForwardACCCCCTATTGAGAGAGATTGCT RNF31 ReverseTGGAGCCTGGGACAGAGG ERα ForwardGCT ACG AAG TGG GAA TGA TGA AAG ERα ReverseTCT GGC GCT TGT GTT TCA AC PS2 ForwardCAT CGA CGT CCC TCC AGA AGA G PS2 ReverseCTC TGG GAC TAA TCA CCG TGC TG Cyclin D1 Forward CAC GCG CAG ACC TTC GT Cyclin D1 Reverse GGG CGG ATT GGA AAT GAA C ADORA1 Forward GGA TCG ATA CCT CCG AGT CA ADORA1 Reverse GAG AAT CCA GCA GCC AGC TA 36B4 Forward GGCGACCTGGAAGTCCAACT 36B4 Reverse CCATCAGCACCACAGCCTTC PKIB Forward CHIP TCA CTG CGG GAT ATT GGC TTA PKIB Reverse CHIP AGC TGG TCT TCT TCC TCC TAA ACT G ADORA1 Forward CHIP GAT GGA TGG GAA CAC ATT GGT ADORA1 Reverse CHIP TGG TGG CGG AGC ACA AA ERα Forward CHIP GGG ATC GCT CCA AAT CGA ERα Reverse CHIP CTT GCC CTG ACA TTG GCT TAA PS2 Forward CHIP CCT CCC GCC AGG GTA AAT AC PS2 Reverse CHIP CCG GCC ATC TCT CAC TAT GAA 18S Forward CHIP GCTTAATTTGACTCAACACGGGA 18S Reverse CHIP AGCTATCAATCTGTCAATCCTGTC Real-time PCR primers used in this study Table S1

9 TCGA KMplot.com All samples Symbolr2r2 P-valueSymbolr2r2 P-value ACE00.0300.37ACE00.2500.00 ADA00.0300.31ADA00.0600.01 ADORA100.0400.19ADORA100.1100.00 APOA100.1700.00APOA100.0800.00 AREG00.0700.05AREGNA AURKA00.0800.01AURKA-0.0901.00 BTG200.0600.08BTG200.1400.00 CCNG200.0200.53CCNG2-0.1001.00 CSF1R00.0100.05CSF1R00.2100.00 CYP1A100.0200.04CYP1A100.2200.00 ESR100.1800.00ESR100.1300.00 GJB200.0000.06GJB2NA GREB100.2100.00GREB100.1100.00 HMOX100.0100.05HMOX100.0100.37 IGFBP400.1400.00IGFBP400.2000.00 KCNMA100.0700.03KCNMA100.0700.00 PDZK100.1800.00PDZK1NA SGK300.0300.34SGK3NA TFF100.2400.00TFF100.0600.00 TGFB300.1000.00TGFB300.1700.00 ACE2-0.1700.00ACE2-0.0100.04 ACPP-0.0100.04ACPP00.1800.00 CAV2-0.3000.00CAV2-0.0801.00 CCNE1-0.1000.00CCNE1-0.0901.00 CD36-0.1600.00CD36-0.0601.00 CDKN1A-0.0200.57CDKN1A00.1400.00 CXCL1-0.1200.00CXCL1-0.0200.05 CXCL12-0.0900.01CXCL1200.0000.45 CXCR4-0.0200.58CXCR4-0.1001.00 CXCR7-0.1700.00CXCR7-0.0300.06 CYP19A1-0.0700.02CYP19A100.1600.00 CYP1B1-0.1800.00CYP1B1-0.0701.00 EGFR-0.2100.00EGFR00.0200.17 EGR3-0.0100.06EGR300.1000.00 NR5A2-0.1400.00NR5A200.1700.00 PPARA-0.2000.00PPARA00.1300.00 REL-0.0700.02REL-0.0100.04 S100A8-0.1500.00S100A8-0.1001.00 TRPV6-0.0900.00TRPV6-0.0300.06 WISP2-0.0300.39 WISP200.0600.01 Table S2 RNF31 expression correlates with expression of known ERα target genes Yellow: p-value < 0.05; Red: positive correlation; Green: negative correlation

Download ppt "Fig. S1 siControl E2 G1: 45.7% S: 26.9% G2-M: 27.4% siER  E2 G1: 70.9% S: 9.9% G2-M: 19.2% G1: 57.1% S: 12.0% G2-M: 30.9% siRNF31 E2 A B siRNF31 siControl."

Similar presentations

Introduction to perl programming: the minimum to know! Bioinformatic and Comparative Genome Analysis Course HKU-Pasteur Research Centre - Hong Kong, China.

Introduction to perl programming: the minimum to know! Bioinformatic and Comparative Genome Analysis Course HKU-Pasteur Research Centre - Hong Kong, China.
Supplementary Figure S1 (A) Change of reporter activity levels after actinomycin D treatment. HEK293T cells were transiently transfected with the reporter.

Supplementary Figure S1 (A) Change of reporter activity levels after actinomycin D treatment. HEK293T cells were transiently transfected with the reporter.
Uses of Cloned Genes sequencing reagents (eg, probes) protein production insufficient natural quantities modify/mutagenesis library screening Expression.

Uses of Cloned Genes sequencing reagents (eg, probes) protein production insufficient natural quantities modify/mutagenesis library screening Expression.
The genetic code.

The genetic code.
Center for Biological Sequence Analysis The Technical University of Denmark DTU Chromatin and Gene Expression in E. coli Dave Ussery Biological Sequence.

Center for Biological Sequence Analysis The Technical University of Denmark DTU Chromatin and Gene Expression in E. coli Dave Ussery Biological Sequence.
Center for Biological Sequence Analysis Prokaryotic gene finding Marie Skovgaard Ph.D. student

Center for Biological Sequence Analysis Prokaryotic gene finding Marie Skovgaard Ph.D. student
Restriction Enzymes Lecture 15: 1 11/20/2006 1. Definition: enzymes that recognize specific double-stranded sequences and hydrolyze the phosphodiester.

Restriction Enzymes Lecture 15: 1 11/20/ Definition: enzymes that recognize specific double-stranded sequences and hydrolyze the phosphodiester.
 -GLOBIN MUTATIONS AND SICKLE CELL DISORDER (SCD) - RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLP)

 -GLOBIN MUTATIONS AND SICKLE CELL DISORDER (SCD) - RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLP)
ATG GAG GAA GAA GAT GAA GAG ATC TTA TCG TCT TCC GAT TGC GAC GAT TCC AGC GAT AGT TAC AAG GAT GAT TCT CAA GAT TCT GAA GGA GAA AAC GAT AAC CCT GAG TGC GAA.

ATG GAG GAA GAA GAT GAA GAG ATC TTA TCG TCT TCC GAT TGC GAC GAT TCC AGC GAT AGT TAC AAG GAT GAT TCT CAA GAT TCT GAA GGA GAA AAC GAT AAC CCT GAG TGC GAA.
Supplementary Fig.1: oligonucleotide primer sequences.

Supplementary Fig.1: oligonucleotide primer sequences.
Gene Mutations Worksheet

Gene Mutations Worksheet
Transcription & Translation Worksheet

Transcription & Translation Worksheet
Crick’s early Hypothesis Revisited. Or The Existence of a Universal Coding Frame Axel Bernal UPenn Center for Bioinformatics Jean-Louis Lassez Coastal.

Crick’s early Hypothesis Revisited. Or The Existence of a Universal Coding Frame Axel Bernal UPenn Center for Bioinformatics Jean-Louis Lassez Coastal.
1 Essential Computing for Bioinformatics Bienvenido Vélez UPR Mayaguez Lecture 5 High-level Programming with Python Part II: Container Objects Reference:

1 Essential Computing for Bioinformatics Bienvenido Vélez UPR Mayaguez Lecture 5 High-level Programming with Python Part II: Container Objects Reference:
In vitro expression of BVDV capsid protein Corpus Christi College, University of Oxford Glycobiology Institute, Department of Biochemistry KOR SHU CHAN.

In vitro expression of BVDV capsid protein Corpus Christi College, University of Oxford Glycobiology Institute, Department of Biochemistry KOR SHU CHAN.
Today… Genome 351, 8 April 2013, Lecture 3 The information in DNA is converted to protein through an RNA intermediate (transcription) The information in.

Today… Genome 351, 8 April 2013, Lecture 3 The information in DNA is converted to protein through an RNA intermediate (transcription) The information in.
Figure S1. Sequence alignment of yeast and horse cyt-c (Identity~60%), green highly conserved residues. There are 40 amino acid differences in the primary.

Figure S1. Sequence alignment of yeast and horse cyt-c (Identity~60%), green highly conserved residues. There are 40 amino acid differences in the primary.
Dictionaries.

Dictionaries.
IGEM 8.3.10 - 8.6.10. Arsenic Bioremediation Possibly finished biobrick for ArsR by adding a RBS and terminator. Will send for sequencing today or Monday.

IGEM Arsenic Bioremediation Possibly finished biobrick for ArsR by adding a RBS and terminator. Will send for sequencing today or Monday.
 The following material is the result of a curriculum development effort to provide a set of courses to support bioinformatics efforts involving students.

 The following material is the result of a curriculum development effort to provide a set of courses to support bioinformatics efforts involving students.

Similar presentations

Ads by Google