1. 1 | Program Name or Ancillary Texteere.energy.gov Water Power Peer Review Environmentally Benign and Permanent Modifications to Prevent Biofouling on Marine and Hydrokinetic Devices Zheng Zhang, Ph.D. Semprus BioSciences 1 Kendall Square. Building 1400, Cambridge, MA 02139 617/577-7755 x213 jonathan.zhang@semprusbio.com September 22, 2011

2. 2 | Wind and Water Power Programeere.energy.gov Purpose, Objectives, & Integration Purpose: Develop environmentally benign and permanent modifications to prevent biofouling on marine and hydrokinetic (MHK) devices. Objectives: Offer improvements and enhancements to MHK systems in the following segments related to biofouling: –Energy efficiency –Reliability –Power quality –Environmental impact –Maintenance costs Integration: Expect to outperform currently used nontoxic underwater coatings in biofouling resistance and be ready for the next stage of development with demonstration in MHK systems.

3. 3 | Wind and Water Power Programeere.energy.gov Technical Approach Design and develop modification systems applicable for permanent anti ‐ fouling modifications. (Planned completion date: 9/30/2011) –Monomer and polymer synthesis –Modification formulation development –Curing/applying method development –Characterization and stability evaluation Demonstrate a broad spectrum of anti ‐ biofouling performance, long ‐ term efficacy and biocompatibility. (Planned completion date: 12/31/2011) –Protein adsorption assay –Bacterial/biofilm assay –Barnacle assay –Biocompatibility evaluation –60 ‐ day field test

4. 4 | Wind and Water Power Programeere.energy.gov Key Issues and Unique Approach Key Issues Broad spectrum of anti-fouling efficacy Mechanical properties, applicability, and compatibility to current underwater coatings Long-term stability and anti-fouling performance Translation to field efficacy Unique Approach Molecular design of betaine-based monomers and polymers to resist bio- fouling. Optimized formulations and applying conditions to achieve high anti-fouling efficacy with mechanical performance and stability Modification and application adaptable to current underwater coating systems. Demonstration of anti-fouling performance with model fouling organisms and a 60-day field test

5. 5 | Wind and Water Power Programeere.energy.gov Plan, Schedule, & Budget Schedule: Initiation date: January 1, 2011 Planned completion date: December 31, 2011 Milestones for FY10 and FY11: –Milestone 1: Design modification systems and synthesize polymers applicable for permanent anti ‐ fouling modifications. Planned completion date: September 30, 2011 –Milestone 2: Demonstrate a broad spectrum of anti ‐ biofouling performance, long ‐ term efficacy, and biocompatibility. Planned completion date: December 31, 2011 Budget: Budget History FY2009FY2010FY2011 DOECost-shareDOECost-shareDOECost-share 160K40K

6. 6 | Wind and Water Power Programeere.energy.gov Settlement of Barnacle Cyprids Protein and Bacteria Reduction Aging Test with Artificial Seawater (ASW) Betaine-Modified Silicone (PDMS) Betaine-modified aluminium panels (4”X8”) for a 60-day field test