1. BIOSAFETY (HEALTH SAFETY) IN THE CLINICAL LAB Dr. Osama h al jiffri

2. Clinical lab technologists are in frequent contact with patient and other biological samples and materials some of which are potentially hazardous to health and life.

3. GENERAL CONSIDERATIONS 1- Before working in the lab, each technologist must be immunized against potentially infective diseases that may be transmitted from patients and biological samples such as; hepatitis, polio, tetanus, tuberculosis (BCG), malaria and cholera (in endemic areas)1- Before working in the lab, each technologist must be immunized against potentially infective diseases that may be transmitted from patients and biological samples such as; hepatitis, polio, tetanus, tuberculosis (BCG), malaria and cholera (in endemic areas) 2- Consider ALL patients specimens are a serious source of infection2- Consider ALL patients specimens are a serious source of infection 3- Strict adherence to safety rules and regulations must be practiced when collecting, handling, transporting and storing patient specimen3- Strict adherence to safety rules and regulations must be practiced when collecting, handling, transporting and storing patient specimen

4. GENERAL CONSIDERATIONS 4- Always wear personal protective materials when collecting, handling, transporting and storing patient specimen. These include; disposable gloves face masks, labcoat, apron or gown, hair cover and shoes.4- Always wear personal protective materials when collecting, handling, transporting and storing patient specimen. These include; disposable gloves face masks, labcoat, apron or gown, hair cover and shoes. 5- NEVER EAT, DRINK, SMOKE, CHEW GUM IN THE LAB5- NEVER EAT, DRINK, SMOKE, CHEW GUM IN THE LAB 6- Never place or store food, drinks, or any material for human consumption (including prescription drugs and medicines) in the lab freezers or fridges6- Never place or store food, drinks, or any material for human consumption (including prescription drugs and medicines) in the lab freezers or fridges 7- NEVER MOUTH PIPETTE7- NEVER MOUTH PIPETTE

5. GENERAL CONSIDERATIONS 8- Always cover wounds or cuts (however small) with proper medical plaster and wear gloves or shoes to cover the whole area.8- Always cover wounds or cuts (however small) with proper medical plaster and wear gloves or shoes to cover the whole area. 9- Never wear the normal head gear (eqal & ghutra) during working in the lab.9- Never wear the normal head gear (eqal & ghutra) during working in the lab. 10- Always wash your hands thoroughly with an antiseptic, bactericidal soap before and after wearing gloves10- Always wash your hands thoroughly with an antiseptic, bactericidal soap before and after wearing gloves

6. GENERAL CONSIDERATIONS 11- Always wash your hands thoroughly with an antiseptic, bactericidal soap before leaving the lab11- Always wash your hands thoroughly with an antiseptic, bactericidal soap before leaving the lab 12- Button up the lab coat while working in the lab12- Button up the lab coat while working in the lab 13- When there is a chance of sample sprays or splashes, then disposable nylon apron or gown must be worn on top of the lab coat together with safety glasses or goggles13- When there is a chance of sample sprays or splashes, then disposable nylon apron or gown must be worn on top of the lab coat together with safety glasses or goggles 14- Never wear the lab coat outside the lab except for work connected with the lab work14- Never wear the lab coat outside the lab except for work connected with the lab work

7. GENERAL CONSIDERATIONS 15- Handle needles and sharp objects with great care to avoid injury15- Handle needles and sharp objects with great care to avoid injury 16- Dispose off needles and sharp objects in the sharps disposal bin16- Dispose off needles and sharp objects in the sharps disposal bin 17- Ensure that the lab has eye-wash kit and solution17- Ensure that the lab has eye-wash kit and solution 18- The lab must have a suitable antiseptic, bactericidal, fungicidal and antiviral solution (Na hypochlorate; colorox, cidex or any suitable bleach) to remove and decontaminate spills from specimens18- The lab must have a suitable antiseptic, bactericidal, fungicidal and antiviral solution (Na hypochlorate; colorox, cidex or any suitable bleach) to remove and decontaminate spills from specimens

8. GENERAL CONSIDERATIONS 19- The lab must have a suitable antiseptic, bactericidal, fungicidal and antiviral spray [Na hypochlorate (COLOROX); Lysol, Medispray] to remove and decontaminate spills from specimens on bench surfaces19- The lab must have a suitable antiseptic, bactericidal, fungicidal and antiviral spray [Na hypochlorate (COLOROX); Lysol, Medispray] to remove and decontaminate spills from specimens on bench surfaces 20- The lab must have updated spill kits for chemical spillage (acid spills, alkali spills, mercury spills, toxic materials spills)20- The lab must have updated spill kits for chemical spillage (acid spills, alkali spills, mercury spills, toxic materials spills) 21- A staff common room must be available which could be used for injured or ill staff member21- A staff common room must be available which could be used for injured or ill staff member

9. PROCEDURE FOR HANDLING A PATIENT SPECIMEN OF KNOWN POTENTIAL INFECTION HAZARD 1- All materials for collection must be sterile and disposable1- All materials for collection must be sterile and disposable 2- Wear proper protection gear2- Wear proper protection gear 3- Wash your hands thoroughly with an antiseptic, bactericidal soap before and after wearing gloves3- Wash your hands thoroughly with an antiseptic, bactericidal soap before and after wearing gloves 4- Collected specimen must be placed in tightly sealed double bag4- Collected specimen must be placed in tightly sealed double bag

10. PROCEDURE FOR HANDLING A PATIENT SPECIMEN OF KNOWN POTENTIAL INFECTION HAZARD 5- Both the specimen container and the bag must be clearly labelled with proper warning sign e.g. BIOLOGICAL HAZARDHEPATITIS RISK OR HIV RISK5- Both the specimen container and the bag must be clearly labelled with proper warning sign e.g. BIOLOGICAL HAZARDHEPATITIS RISK OR HIV RISK 6- The request form should be placed in another bag accompanying the specimen and it must bear the same warning sign as the specimen6- The request form should be placed in another bag accompanying the specimen and it must bear the same warning sign as the specimen 7- All needles, syringes, gloves, masks, aprons, gowns, hair cover and overshoes that were used during collection must be placed in a special biohazard bag which bears proper warning and sealed properly and taken for incineration7- All needles, syringes, gloves, masks, aprons, gowns, hair cover and overshoes that were used during collection must be placed in a special biohazard bag which bears proper warning and sealed properly and taken for incineration

11. PROCEDURE FOR HANDLING A PATIENT SPECIMEN OF KNOWN POTENTIAL INFECTION HAZARD 8- When the specimen arrives in the lab, it must be placed in the safety cabinet until analysis8- When the specimen arrives in the lab, it must be placed in the safety cabinet until analysis 9- The specimen may be analyzed by its own in separate run or may be placed to be the last in a normal routine run9- The specimen may be analyzed by its own in separate run or may be placed to be the last in a normal routine run 10- In the analyzer it must be followed by a hypochlorate (or any disinfectant) wash10- In the analyzer it must be followed by a hypochlorate (or any disinfectant) wash

12. PROCEDURE FOR HANDLING A PATIENT SPECIMEN OF KNOWN POTENTIAL INFECTION HAZARD 11- All pipettes, centrifuge, analyzers and any other device used for the specimen must be thoroughly disinfected and cleaned with hypochlorite or other strong disinfectant11- All pipettes, centrifuge, analyzers and any other device used for the specimen must be thoroughly disinfected and cleaned with hypochlorite or other strong disinfectant 12- After finishing with the specimen, place it in a double plastic bag which must then be tightly sealed and labelled as BIOHAZARD and taken for incineration12- After finishing with the specimen, place it in a double plastic bag which must then be tightly sealed and labelled as BIOHAZARD and taken for incineration 13- If storage of the specimen is required, then the tightly sealed double bag must be placed in a sealed rigid plastic box then place in a specified area in the fridge or freezer13- If storage of the specimen is required, then the tightly sealed double bag must be placed in a sealed rigid plastic box then place in a specified area in the fridge or freezer

13. سيرة ذاتية أخرى ● نماذج منشورات FID العنوانالوصف تاريخ الإضافة 6370 فيروسات محاضرة رقم (1) Papilloma viruses and Poxviruses 11/7/200 8 5:43:56 PM تحميل الملف 6373 فيروسات محاضرة رقم (2) Viral Agents Causing Gastroent eritis 11/7/200 8 5:53:55 PM تحميل الملف 13534 BIOSAFE TY-01 12/26/20 08 5:15:12 PM تحميل الملف 13537 BIOSAFE TY-02 12/26/20 08 5:39:23 PM تحميل الملف الملفات www.kau.edu.sa/ojiffri