Presentation is loading. Please wait.

Presentation is loading. Please wait.

Genetic Reduction of Inositol Triphosphate (InsP 3 ) Increases Tolerance of Tomato Plants to Oxidative Stress Mohammad Alimohammadi 1, Mohamed H. Lahiani.

Published byBriana Elliott Modified over 8 years ago

Similar presentations

Presentation on theme: "Genetic Reduction of Inositol Triphosphate (InsP 3 ) Increases Tolerance of Tomato Plants to Oxidative Stress Mohammad Alimohammadi 1, Mohamed H. Lahiani."— Presentation transcript:

1 Genetic Reduction of Inositol Triphosphate (InsP 3 ) Increases Tolerance of Tomato Plants to Oxidative Stress Mohammad Alimohammadi 1, Mohamed H. Lahiani 1 and Mariya V. Khodakovskaya 1 * 1 Department of Biology, University of Arkansas at Little Rock *Author for correspondence: mvkhodakovsk@ualr.edumvkhodakovsk@ualr.edu Tel: 501- 683-2656 Fax: 501-569-3271

2

3 Figure S1: Transcript profile of genes expressed differently in InsP 5-ptase transgenic line (L7) and with wild- type (WT) exposed to continuous light (50 µmol m -2 s -1 light intensity at 35 °C) for 7 days. Genes are clustered by hierarchical clustering (Euclidean distance). Two independent biological replicates for each plant line are presented here.

4 a b Figure S2: Relative transcript abundance of (a) PR protein (Les.3408.1.S1_at) and (b) wound- induced proteinase inhibitor II prepeptide (Les.1675.1.S1_at) in tomato wild-type (WT) and InsP 5-tase transgenic line L7 exposed to continuous light (50 µmol m-2 s-1 light intensity at 35 °C) for 7 days. Gene expression was analyzed by qRT-PCR. Results are demonstrated as the average of two independent biological replicates. Relative expression levels were normalized to an internal standard (18S transcript) for each treatment. Bars represent standard error (SE) of means.

Download ppt "Genetic Reduction of Inositol Triphosphate (InsP 3 ) Increases Tolerance of Tomato Plants to Oxidative Stress Mohammad Alimohammadi 1, Mohamed H. Lahiani."

Similar presentations

35S P.tAPX or GUS intronNOS T. G 10-90 P.XVET3A Lex A P.ccdB G 10-90 P.XVET3A Lex A P. tAPX or GUS intron Xba I pGWB80/tAPX (or GUS) pMDC7 A B C pMDC7/tAPX.

35S P.tAPX or GUS intronNOS T. G P.XVET3A Lex A P.ccdB G P.XVET3A Lex A P. tAPX or GUS intron Xba I pGWB80/tAPX (or GUS) pMDC7 A B C pMDC7/tAPX.
Figure S1. Genomic PCR of in vitro potato plants transformed with StPTB1 prom (top) and StPTB6 prom (bottom) constructs using nptII-specific primers. Thirty.

Figure S1. Genomic PCR of in vitro potato plants transformed with StPTB1 prom (top) and StPTB6 prom (bottom) constructs using nptII-specific primers. Thirty.
Supplemental figure 1: Correlation coefficients between signal intensities from biological replicates of wild.

Supplemental figure 1: Correlation coefficients between signal intensities from biological replicates of wild.
Supplementary Fig. S1: Hierarchical clustering of AUX/IAA genes, showing significant differential expression under at least one abiotic stress condition.

Supplementary Fig. S1: Hierarchical clustering of AUX/IAA genes, showing significant differential expression under at least one abiotic stress condition.
Figure S1. Alignment of identified AtMYB93/92/53 homologues in land plants, used to infer the phylogeny in Figure 1B. Supporting information Figs S1-S7.

Figure S1. Alignment of identified AtMYB93/92/53 homologues in land plants, used to infer the phylogeny in Figure 1B. Supporting information Figs S1-S7.
Figure SOM1. Functional roles of the genes affected in zmet2-m1 mutants. Although the genes localized on the intracellular membranes were slightly over-represented.

Figure SOM1. Functional roles of the genes affected in zmet2-m1 mutants. Although the genes localized on the intracellular membranes were slightly over-represented.
Gene expression (signal intensity) Control Osmotic Salt Drought Root 0 200 400 600 800 Control 0.5 13 612 24 Gene expression (signal intensity) Treatment.

Gene expression (signal intensity) Control Osmotic Salt Drought Root Control Gene expression (signal intensity) Treatment.
Supplementary Fig. S1: GO classification of 2,318 differentially expressed genes during fruit ripening in tomato. The genes which show differential expression.

Supplementary Fig. S1: GO classification of 2,318 differentially expressed genes during fruit ripening in tomato. The genes which show differential expression.
Supplementary data 2: Reproducibility of metabolite MSI Reproducibility of MSI data was evaluated by mean ± standard deviation (5 biological replications).

Supplementary data 2: Reproducibility of metabolite MSI Reproducibility of MSI data was evaluated by mean ± standard deviation (5 biological replications).
Figure S1 Figure S1. European corn borer (ECB) oral secretions (OS) did not induce the expression of proteinase inhibitors in tomato and maize. (a) Relative.

Figure S1 Figure S1. European corn borer (ECB) oral secretions (OS) did not induce the expression of proteinase inhibitors in tomato and maize. (a) Relative.
Supplemental Figure 1. The cell death phenotype of fhy3 far1 double mutants. A. The cell death phenotype of fhy3-4 far1-2 mutant plants under LD conditions.

Supplemental Figure 1. The cell death phenotype of fhy3 far1 double mutants. A. The cell death phenotype of fhy3-4 far1-2 mutant plants under LD conditions.
Supplementary Material

Supplementary Material
S8 Figure. Expression of genes determined by qPCR matches expression determined from transcriptome assembly. Genes encoding adenylate cyclase, triacylglycerol.

S8 Figure. Expression of genes determined by qPCR matches expression determined from transcriptome assembly. Genes encoding adenylate cyclase, triacylglycerol.
Additional file 8: Estimation of biological variations

Additional file 8: Estimation of biological variations
Blue 10 WT OX OX21 BZS1-OX A B Blue Fluence level (μmol/m2/s)

Blue 10 WT OX OX21 BZS1-OX A B Blue Fluence level (μmol/m2/s)
Microtubule perturbations affect pathway variability η2(P) and transmitted signal P at or upstream of the Ste5 recruitment step Microtubule perturbations.

Microtubule perturbations affect pathway variability η2(P) and transmitted signal P at or upstream of the Ste5 recruitment step Microtubule perturbations.
Fig. S12. Hierarchical tree graph of overrepresented GO terms in biological process category in 18O w.r.t. WT under 100mM NaCl at 24 h stress comparative.

Fig. S12. Hierarchical tree graph of overrepresented GO terms in biological process category in 18O w.r.t. WT under 100mM NaCl at 24 h stress comparative.
Volume 7, Issue 2, Pages (February 2014)

Volume 7, Issue 2, Pages (February 2014)
IGF-1 does not moderate the time-dependent transcriptional patterns of key homeostatic genes induced by sustained compression of bovine cartilage  C.A.

IGF-1 does not moderate the time-dependent transcriptional patterns of key homeostatic genes induced by sustained compression of bovine cartilage  C.A.
(x 10-3) (x 10-4) GST P450 3 12 2 8 1 4 WT mock WT #15 WT mock WT #15

(x 10-3) (x 10-4) GST P WT mock WT #15 WT mock WT #15

Similar presentations

Ads by Google