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PCR condition EF taq Tm : 45~60 ℃ Template : gDNA 2 95 ℃ 2 min 95 ℃ 30 sec Tm 20 sec (30cycles) 72 ℃ 4 min 72 ℃ 5 min 4 ℃ 연대의대 소아과 - PCR results (gDNA.

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Presentation on theme: "PCR condition EF taq Tm : 45~60 ℃ Template : gDNA 2 95 ℃ 2 min 95 ℃ 30 sec Tm 20 sec (30cycles) 72 ℃ 4 min 72 ℃ 5 min 4 ℃ 연대의대 소아과 - PCR results (gDNA."— Presentation transcript:

1 PCR condition EF taq Tm : 45~60 ℃ Template : gDNA 2 95 ℃ 2 min 95 ℃ 30 sec Tm 20 sec (30cycles) 72 ℃ 4 min 72 ℃ 5 min 4 ℃ 연대의대 소아과 - PCR results (gDNA 2) 4 Kb · gDNA 2 를 template 로 하여 4kb size 의 PCR product 를 얻어 sequencing 의뢰함 Tm: 45 / 47.5 / 51.4 / 53.9 / 56 / 58.8 ( ℃ )

2 PCR condition EF taq Tm : 45~56 ℃ Template : gDNA 1 95 ℃ 2 min 95 ℃ 30 sec Tm 20 sec (30cycles) 72 ℃ 4 min 72 ℃ 5 min 4 ℃ 4 Kb · gDNA 1 을 template 로 하여 4kb size 의 PCR product 를 얻어 sequencing 의뢰함 연대의대 소아과 - PCR results (gDNA 1) Tm: 51.6 / 53.1 / 54.3 / 55.2 ( ℃ )

3 연대의대 소아과 - PCR results gDNA 1gDNA 2

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