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1 PCR RESULTS! Josef Lautin Erica Forsberg Zdravka Boskovic Henrik Kleinhans Adam Kundurakis Håkan Lundberg
2 First PCR run 25 cycles (30 sec + 30 sec + 1 min ) + 5 min 95 °C + 65 °C + 72 °C Polymerase: Taq Results: None
3 Results of first PCR run Our vector (red) and lack of PCR product (orange)
4 Second PCR run 25 cycles 1 min + (15 sec + 15 sec + 15 sec) 95 °C + 60 °C + 72 °C Polymerase: DreamTaq Results: Good
5 Results of second PCR run Our second PCR run result
6 Results of third PCR run
7 Results discussion First 3 wells (red) were from our own colonies, 4 th one (orange) from Lasse. 3 rd one, not the expected DNA-fragment.
8 What might have gone wrong First PCR might have been a pipetation error. Third PCR, third colony: The PCR prep. might have failed, since if there was a colony it should have been ok.
PCR condition EF taq Tm : 45~60 ℃ Template : gDNA 2 95 ℃ 2 min 95 ℃ 30 sec Tm 20 sec (30cycles) 72 ℃ 4 min 72 ℃ 5 min 4 ℃ 연대의대 소아과 - PCR results (gDNA.
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