1. BIO 185 THE BIOLOGY OF THE CELL Winter 2014 Instructors: Walter J. Bruyninckx Office: Science Center,217 Phone - office: 866-7242 - home: 866-4522 (before 9:00 PM) e-mail:bruyninx@hanover.edubruyninx@hanover.edu Office Hours: Monday 9:00 - 10:00 am Tuesday12:00 - 5:00 pm Wednesday 12:00 - 4:00 pm Thursday 12:00 - 3:00 am Friday9:00 – 10:00 am

7. Venus in front of the mirror (1613)

8. Bacchus (1638)

9. The Union of Earth and Water (1618)

17. Bio 185

18. Bio 185. Cell and Molecular Biology  Molecules of the living material  Cellular organization of living material  Energy metabolism  Molecular biology: central dogma  Cancer biology

19. Bio 185 Cell and Molecular Biology ACTIVITIES: 1.Lectures 2.Preparing a Notebook 3.Study 4.Readings 5.Lab 6.Exams.

20. DueAuthors Scientific America TitlePages 1/11 Gerstein Mark and Levitt Michael November 1998 Simulating water and molecules of life100-105 1/18Welch William J.May 1993How cells respond to stress56-64 2/01 Goldberg Alfred L., Elledge Stephen J. and Harper J. Wade January 2001 The cellular chamber of doom68-73 2/15 Rothman James E. and Orci Lelio March 1996 Budding vesicles in living cells70-75 2/22McCarthy Richard E1985 H+ ATPases in oxidative and photosynthetic phosphorylation BioScienc e 35 p7- 30 3/15 Lau Nelson C. and Bartel David P. August 2003 Censors of the genome34-41 3/22Ast GilApril 2005The alternative genome58-65 4/05 Scott John D. and Pawson Tony June 2000Cell communication: the inside story72-79 4/12Weinberg Robert A. September 1996 How cancer arises62-70

21. ExamdateLecturespoints% Exam 101/25The molecular basis of Life (Lecture1-11)8013.33 Exam 202/08Cell structure (Lecture 12-20)8013.33 Exam 303/08Energy metabolism (Lecture 21-28)8013.33 Exam 403/28Molecular Biology (Lecture 29-40)8013.33 Exam 504/15Cancer (Lecture 40-49)8013.33 Cmprh. ex04/15Lectures (1-49)50 8.33 Lab exam04/0950 8.33 Lab reports50 8.33 Readings50 8.33 Total600 Bio 185. Molecular Cell Biology Grading

23. Bio 185 Cell and Molecular Biology http://vault.hanover.edu/~bruyninx/  Bio185Bruyninckx Bio185Content Bio185Exercises Bio185LabData Bio185PowerPoint Bio185ReadingAssignments Bio185Syllabus

24. Bio 185. Molecular Cell Biology Course policies

25. 3. Course policies. 0. Do NOT wear a baseball cap (in whatever direction) in class or lab. 1. Your active and attentive presence in class and lab is expected. 2. Any work handed in after the time is due will not be graded. 3. All written material turned in for credit must be typewritten and paper- copy (I am sorry, we cannot be your printer). Multiple pages need to be stapled together! 4. All writing that is not your own independent work must be properly referenced. Please refer to the Student Handbook (pages 2 to 3) and the policy statement on academic dishonesty. 4. There is no opportunity to make up quizzes, laboratories, assignments. 5. For safety reasons, eating, drinking and smoking are not permitted in the laboratory or class. Shoes must be worn at all times in the laboratory, as there may be broken glass and other hazardous material on the floor. 6. Turn off your cell phone or other similar devices when in class, lecture, or study session. 7.Limit use of a computer in class or lab to the scheduled class activities. 8.Please, no smelly food!

26. WkDateTitleTopic 101/08IntroductionTopic-1 201/15Laboratory toolsTopic-1 301/22Introduction to bio-moleculesTopic-2 401/29Characterization of enzymes-1Topic-3 502/05Characterization of enzymes-2Topic-3 602/12Cell membrane and osmosisTopic 4 702/19Cell StructureTopic 4 803/05Respiration and fermentationTopic 5 903/12PhotosynthesisTopic 6 1003/19Analysis of proteinsTopic 9 1103/26DNA and Cell cycleTopic 7, 8 1204/02Gene regulation: lac operonTopic 10 1304/09Lab Exam

27. LAB REPORT FORMAT: (1 report per group!) LAB TITLE: Properties and Function of Enzymes. Experiment 1. Goal: one sentence. Method: Relevant. Results: (based on class data). A (mean and SEM) is significantly different (P<0.05) from B (mean and SEM). Conclusion: A is different from B. Explanation.

29. Figure 1. Standard curve of yellow dye at 420 nm. We measured the optical density at 420 nm of four concentrations of yellow dye (1, 2, 3, and 4 drops per sample) using a Spectronix 21 spectrophotometer. The OD of Standard 1 ((0.217 ± 0.0070) was significantly smaller (P<0.05, Student’s t-test) than that of Standard 2 (0.410 ± 0.0057), the OD of Standard 2 was significantly smaller (P<0.05) than that of Standard 3 (0.619 ± 0.0045) and the OD of Standard 3 was significantly smaller (P<0.05) than that of Standard 4 (0.806 ± 0.0087). We calculated the standard curve (linear regression y = 0.1977x + 0.0186, R 2 = 0.9996).We measured the optical density at 420 nm of an unknown concentration of yellow dye (OD equals 0.699 ± 0.0054) and used the standard curve to determine its concentration (3.44 drops per sample).