Presentation on theme: "Linkage and Genetic Mapping"— Presentation transcript:
1Linkage and Genetic Mapping Lecture 1Human Molecular Genetics (Strachan and Read) Chapters 4, 13, 14
2What are genetic diseases? There are two basic types 1. Simple Mendelian (easy to analyse)e.g. Huntington's disease, cystic fibrosis, Duchenne muscular dystrophy follow a simple pattern of inheritance (e.g. autosomal dominant, autosomal recessive, X-linked recessive).There is a complete correlation between genotype and phenotype. If you've got the mutant gene, you'll get the disease.2. Complex or multifactorial (hard to analyse)e.g. many common diseases, such as cancer, asthma, schizophrenia, hypertension, heart disease.The risk of getting the disease is modified by individual's genotype.Evidence for "genetic-ness" of a disease is expressed as l = (risk to 1st degree relatives of patient)/(population risk). For type I diabetes, l = 15 (6%/0.4%).Other factors, especially other genes and environment, also influence risk of getting disease.
3Strategies for cloning a disease gene If you know the biochemical basis of the disease, e.g. there is an enzyme missing from a biochemical pathway, you can go straight to the gene that codes for the enzyme/protein. This called functional cloningIf you have some idea of the pathological basis of the disease, or if there is a similar animal or human disease for whose basis is known, you might be able to guess what the gene might be and test that gene directly in patients: the candidate gene approachIf the only thing you know about the disease gene is its location in the genome from linkage studies, the approach used is called positional cloningPositional cloning can be based on translocations or deletions that disrupt the gene and cause the phenotype (rare), or on linkage mapping in families
4The main questions that have to be answered when planning the strategy What type of inheritance? Simple (which mode?) or complex?How to search? Can you guess at a candidate gene? If so, test it directly in patients and controls to see if a mutation is associated with disease stateAre families available? If so can use the genetic linkage approach - study the inheritance of polymorphic DNA sequences, see if any segregate with the disease. This identifies candidate region of genome.
7Linkage and recombination Mapping disease genes in humans is done by using DNA polymorphisms,These polymorphisms can be genotyped using simple laboratory techniques, mostly based on PCR.In a simple genetic disease polymorphisms are studied in family members to find genetic linkage.If the polymorphism is close to the disease gene on the chromosome there is a low chance of recombination at meiosis and linkage is observed.If the polymorphism and disease gene are far apart or on different chromosomes, linkage is not observed.
9Recombination fractions A recombination event gives ½ parental type (P) and ½ recombinant type (R) offspringRecombination fraction RF = R/R+PRF is between 0 and 0.5 (0 and 50%)The closer together the genes are, the smaller is RFRF = 0.5 for unlinked genes (very far apart or on different chromosomes)
12Lod scoresExperimental animals such as mice or fruit-flies produce large numbers of offspring, so can estimate RF very accurately.Human families only produce small numbers of children.To get statistically significant evidence for linkage, combine evidence from many familiesA complex mathematical procedure, implemented by computer software, is used to generate "Lod scores".Lod score is a statistic that describes the strength of evidence for linkage, at any chosen value of the RF, given the family data available.
14A lod score of 3 or more is considered good evidence for linkage. A lod score of -2 or less is evidence against linkage.Values between -2 and 3 are inconclusive and indicate that more data must be obtained.
15Multipoint linkage mapping Using 1000s of markers, genetic maps have been constructed across the whole genomeMultipoint mapping uses several markers at once to localise a disease gene relative to the other markers in the mapMore efficient process than using one marker at a time
16Multipoint linkage mapping Fig 13.8 from Strachan and Read (3rd edition)