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My Excel Macro Based on macro by Frank Millenaar (Utrecht)Frank Millenaar Selects genes of interest from Mas5 output spreadsheet Visualises expression.

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Presentation on theme: "My Excel Macro Based on macro by Frank Millenaar (Utrecht)Frank Millenaar Selects genes of interest from Mas5 output spreadsheet Visualises expression."— Presentation transcript:

1 My Excel Macro Based on macro by Frank Millenaar (Utrecht)Frank Millenaar Selects genes of interest from Mas5 output spreadsheet Visualises expression profiles as combined: data-tables heat-maps Can adapt for different combinations of chips to give different profiles Eg a range of organs. Similar in function to GenevestigATor ….but me if you want to have a go.

2 Input Unformatted NASCAffy (MAS5) output –All 23,000 genes from ATH1 chip –For each gene SpotID Probe Name Gene Name Description Process URL For each array –Signal value –Detection Call –Detection P Value Each replicate chip is separate (3) columns –IE to much data for a human mind to see patterns! List of genes of interest

3 Data for our chosen genes only Can use different chip sets –e.g. organs –e.g. treatments Replicate chips can be meaned For each gene, only: Gene Name For each chip –Signal value (Description) (Process) Signal values colour-coded for visual impact = Heat map output chips genes annotation


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