1. Dr.Arnolds Ubelis (arnolds@latnet.lv) The project: “DEVELOPMENT OF NANOTECHNOLOGY BASED BIOSENSORS FOR AGRICULTURE – BIOSENSORS-AGRICULT” PIRSES-GA-2012-318520 Duration 01.09.2012-31.08. 2016 Budget 292,600.00 Euro PEOPLE, MARIE CURIE ACTIONS Call: FP7-PEOPLE-2012-IRSES International Research Staff Exchange Scheme

2. Dr.Arnolds Ubelis (arnolds@latnet.lv) Coordinator Dr.Arnolds Ubelis arnolds@latnet.lv Association FOTONIKA-LV University of Latvia Scientific secretary Dr.Roman Viter Viter_r@mail.ru Technical assistant – Darta Ubele ( Darta.Ubele@gmail.com).Ubele@gmail.com

3. Dr.Arnolds Ubelis (arnolds@latnet.lv) EU Project Officer DAHON OLIVIER European Commission Research Executive Agency Unit P2 COV2 14/52 B-1049 Brussels/Belgium +32 2 296 4372, olivier.dahon@ec.europa.eu

4. Dr.Arnolds Ubelis (arnolds@latnet.lv)ConsortiumParticipant Legal entity Department 1. Riga, Latvia University of Latvia, LU Association FOTONIKA-LV, (http://www.lu.lv/Fotonika-lv/) http://www.lu.lv/Fotonika-lv/ 2. Linköping, Sweden University of Linköping, Sweden Department of Physics, Chemistry and Biology(IFM) (www.ifm.liu.se ) www.ifm.liu.se 3.Montpellier, France CNRS, Montpellier, France European Institute of Membranes (www.iemm.univ-montp2.fr) 4. Kyiv, Ukraine National University of Life and Environmental Sciences Department of Molecular Biology, Microbiology and Biosecurity (http://nubip.edu.ua/en/) 5. Odessa, Ukraine Odessa National I.I. Mechnikov University Faculty of Physics, Faculty of Biology and Faculty of Chemistry (www.onu.edu.ua,) www.onu.edu.ua 6.Minsk, Belarus National Academy of Sciences of Belarus Institute of Biophysics and Cell Engineering of NASB, (http://ibp.org.by/en/)

5. Dr.Arnolds Ubelis (arnolds@latnet.lv) Exchange scheme EU Non-EU Staff exchange Research teams will implement:: -experimental work; -seminars, workshops; -summer school, conferences; -research training days. Benefits and outcomes : -scientific publications; -knowledge transferre; -ideas for new projects; - new proposals.

6. Dr.Arnolds Ubelis (arnolds@latnet.lv) Mutual Exchange program:   Scholars go abroad either as Experts (senior researchers ) or as Trainees (young researchers) for a period of ca. three months;   Participating in research;   Teaching or research training;   Additional input to this IRSES research project, Master Thesis, PhD thesis or similar projects on a biosensors activity, invited lectures; Exchange balance:   EU --- non-EU countries: in total ca. 53 PM spent abroad;   Non –EU --- EU countries: in total ca. 101 PM spent in EU. Time frame for staying abroad preferably: Minimum 1 month, maximum 12 months per researcher (more than 1 location possible)

7. Dr.Arnolds Ubelis (arnolds@latnet.lv) Totally for 4 project’s years - 154 months/visits = 292 600 € The first reporting period (1-24months) - 96 months/visits = 182 400 € The second reporting period (24-48months) - 58 months/visits = 110 200 € Participant Number of months/ visits x € 1. Riga, Latvia 30 x 1900 = 57 000 € 2. Linköping, Sweden 16 x 1900 = 30 400 € 3. Montpellier, France 7 x 1900 = 13 300 € 4. Kyiv, Ukraine 31 x 1900 = 58 900 € 5. Odessa, Ukraine 39 x 1900 = 74 100 € 6.Minsk, Belarus 31 x 1900 = 58 900 €

8. Dr.Arnolds Ubelis (arnolds@latnet.lv) Table 2: List of Work Packages Work package n° Work package title Involved teams (lead team in bold) Start month End mont h 1 Design and investigations of biosensitive layers Riga, Montpellier, Linköping, Odessa, Minsk, Kiev. 120 2 Technology development of optical fiber and on chip sensor systems Riga, Montpellier, Linköping, Odessa, Minsk, Kiev. 1227 3 Theoretical modelling of surface interaction between biomolecules and biosensor surface Riga, Montpellier, Linköping, Odessa, Minsk, Kiev. 1948 4 Sensitivity measurements of optical fiber and on-chip laboratory made biosensors to different toxins Riga, Montpellier, Linköping, Odessa, Minsk, Kiev. 3048

9. Dr.Arnolds Ubelis (arnolds@latnet.lv) Summer Schools   The first summer school   Location: Riga, Latvia   Time: end of August 2013   The second summer school   Location: Odessa, Ukraine   Time: beginning of June 2014   The third summer school   Location: Odessa, Ukraine   Time: end of August 2016 Modules: (suggestions)   Methods of academic research and writing for PhDs   “state-of-the-arts   Theory of the scientific fields   Experimental methods   Data processing (error limits and systematic mistakes)

10. Dr.Arnolds Ubelis (arnolds@latnet.lv) Conference ‘Biosensors for agriculture’ Responsibility ONU Where Odessa, Ukraine When August 2016 In cooperation with 3DOXIDES (FR), WU&Surfix (NL), Ldiamon (ES), Sud-West photonics (GE) Topic(s): Nanomaterials and Methods for detection of biomolecules Participants besides project parnters EU, USA and Asia Audience Researchers, students, PhD students, SMEs community

11. Dr.Arnolds Ubelis (arnolds@latnet.lv) Timeframe, Milestones, Reporting:   Interim report project months 24th;   Milestones: 1) 1)Proposal of algorithms for data management of biosensing measurements. Draft report or draft of publication. 2) 2)Insight and systematisation of state-of the-art knowledge on application of nanomaterials in biosensors 3) 3)Survey of possibilities and elaboration of plans for field experiments to investigate processes on the surface of biosensitive layers.

12. Dr.Arnolds Ubelis (arnolds@latnet.lv) Activities 2012 Project months 1 to 4; a) a)Planned – to start secondment visits in November; b) b)Delayed - due to readiness of consortia to susstain the success by new complementary projects: - involvement of consortium members in writing and submition FP7 –PEOPLE-2013 – ITN project proposal on 22.11.2012: METONANOSENS’ Nr. ID 607534) - involvement of consortium members in writing and submision of FP7 –PEOPLE-2013 –IAPP project proposal on 16.01.2013: Nr. METOXNANOBIO Nr. ID612325 - overestimation of capacity to deal with formalities; - other reasons: annual reporting, exams and re- asignement of contracts in non EU institutions. C) C)Activities of consortium performed in addition beyond planned: see details in slides Nr. 29, 31, 34

13. Dr.Arnolds Ubelis (arnolds@latnet.lv) Short referencies to the Scientific Content of the project in the next slides

14. Dr.Arnolds Ubelis (arnolds@latnet.lv) The Project Concept Biotechnology Nanomaterials Optical technology Biosensors: Application in agriculture

15. Dr.Arnolds Ubelis (arnolds@latnet.lv) Needs for agriculture  -toxins, plant and animal diseases  -rapid express methods of diagnostics  -selectivity to one sort of biomolecules  -low cost of monitoring devices Biosensors – are the solution

16. Dr.Arnolds Ubelis (arnolds@latnet.lv) Biosensor definition (by Wikipedia)  A biosensor is an analytical device for the detection of an analyte that combines a biological component with a physicochemical detector that cannot detect. It consist of:  the sensitive biological element (biological material, e.g. tissue, microorganisms, organelles, cell receptors, enzymes, antybodies, nucleic asids, etc)  the transducer or the detector element (works in a physicochemical way; optical, piezoelectric, electrochemical, etc.) that transforms the signal resulting from the interaction of the analyte with the biological element into another signal (i.e., transducers) that can be more easily measured and quantified;  biosensor reader device with the associated electronics or signal processors that are primarily responsible for the display of the results in a user-friendly way.

17. Dr.Arnolds Ubelis (arnolds@latnet.lv) Advantages of nanomaterials  -low dimensions  -quantum confinement effects  -high surface area  -affinity to bio molecules  -many methods of depositions

18. Dr.Arnolds Ubelis (arnolds@latnet.lv) Optical transducers Indifferent to electromagnetic fields:  Stable in aggressive environment;  Precise measurement;  Low cost and small size (optical fibers);  Able to create network and distance sensing.

19. Dr.Arnolds Ubelis (arnolds@latnet.lv) Who will participate  Experienced researchers (professors, senior researchers, PhD holders with experience more than 4 years);  Early stage researchers (experience less than 4 years after obtaining MS degree);  Managers, engineers, programmers and technical staff

20. Dr.Arnolds Ubelis (arnolds@latnet.lv) Implementation of the project: Linköping Kiev Odessa Minsk -Deposition of metal oxide nanostructures with CVP method; -Structural, optical and electrical characterization; -Bio fictionalization of nanostructures.

21. Dr.Arnolds Ubelis (arnolds@latnet.lv) Riga Kiev Odessa Minsk -Characterization of optical fiber biosensors; -Characterization of biochips. Implementation of the project:

22. Dr.Arnolds Ubelis (arnolds@latnet.lv) Implementation of the project: Montpellier Kiev Odessa Minsk -Deposition of metal oxide nanostructures with ALD method; -Structural, optical and electrical characterization; -Bio fictionalization of nanostructures.

23. Dr.Arnolds Ubelis (arnolds@latnet.lv) Logo – main and addition al modifica tions ideas