1. 38166-06(9/27/06) Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

2.  Arrowhead education center - Okefenokee Joe  black rat snake: Elaphe obsoleta obsoleta  http://www.okefenokeejoe.com/ http://www.okefenokeejoe.com/ http://www.youtube.com/watch?v=_U2 knmkrKA0 http://www.youtube.com/watch?v=_U2 knmkrKA0 “Okefenokee Joe sings kin to the wind” Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

3.  2 masses: The masses first appeared in August of 2006 and grew very rapidly..  Ventral mandible  Dorsolateral to the eye  DX: Abscess, granuloma, lymphoma/neoplasia  Surgical excision  Histopathology and culture Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

7.  Subcutaneous tissue, Granulomas, Multifocal to coalescing, with Intralesional hyphae and arthroconidiating hyphae Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

8.  Pantoea agglomorans  Enterococcus fecalis  Staphylococcus intermedius Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

9.  Sabouraud’s Dextrose agar  Mycobiotic agar  Incubate at 28- 30 o C for four weeks  Examine twice a week for growth Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

10. “ MYCOLOGY MESS” Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

13.  Macroscopic  Color: wide range  Size: wide range  Texture: wide range  Microscopic- LPCB  Inconsistency in use of terms to describe the microscopic morphology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

19.  Isolate forwarded to The University of Texas Health Science Center at San Antonio for further morphological Characterization  “Chrysosporium anamorph of Nannizziopsis Vriesii” Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

20.  independently populating organism with different morphology: Same Biochemical and physiological properties  Anamorph: asexually propagating thallus  Teleomorph: Sexuallly propagating thallus  Total organism = Holomorph Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

21.  Independently propagating anamorphs  Pseudallescheria boydii  Graphium eumorphum  Scedosporium apiospermum Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

22.  No Matches Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

23.  Morphologic charecterization  Molecular charecterization Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

24.  Chrysosporium ophidiicola Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

26.  Thanks to  VDIL, Bacteriology and Histopathology section  Dr. Deanna Sutton  Dr. Melissa Vanmeter  Professor Guarro Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

27. Questions? Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only