1. The Sargasso Sea “Metagenome”
R/V Weatherbird II

2. The Sargasso Sea “Metagenome”
Slide courtesy of KB Heidelberg

3. Sequence reads
1,662,328
sequence (Mbp)
1,361
Contigs
121,477
256.0
Scaffolds
64,398
span (Mbp)
400.0
Mini-scaffolds
217,015
820.7
353.3
Singletons
215,038
169.9
Total nonredundant sequence (Mbp)
779.2
Scaffolds>3x
333
Span>3x (Mbp)
30.9
Annotated genes
1,001,987
16S rRNA genes
1,164

4. Frequency and diversity of Rhodopsins
How common are these genes, was it an isolated occurrence or are they globally distributed?
Are rhodopsins only found in the SAR86 group (g-Proteobacteria) or are they found in diverse groups?
Proteorhodopsin genes are distributed among divergent marine bacterial taxa.
de la Torre et al PNAS. 100:

5. Venter et al Science. 304:66-74

6. The previous data from Monterey Bay still only suggested the rhodopsins were in the Proteobacteria.
Sargasso Sea data set allowed the expansion of the taxa that contain rhodopsins

7. The previous data from Monterey Bay still only suggested the rhodopsins were in the Proteobacteria.
Sargasso Sea data set allowed the expansion of the taxa that contain rhodopsins
CFB

8. Sorcerer II Global Ocean Sampling Expedition