1. © by Nimmervoll Michaela 10.09.2009 Higher secondary school for chemical ingeneering 1 Nimmervoll The EU-Comenius Life-long-learning-program is financially granted by the European Commission.

2.  History  Properties  Biosynthesis  Adsorption and metabolic processes  Function  Determination 2 Nimmervoll

3.  Remedy for scorbut  1747: James Lind  Ascorbic acid – scorbut  1932: Waugh & King:  Hexuronacid  1933: Harworth & Szent-Györgyi:  Ascorbic acid 3 Nimmervoll

4.  Withe crystalline substance  Molecular weight:  179 g/mol  Melting point:  190°C  Water dissolves  Effectivity:  D-Glucoascorbine acid  Vitamine A, E, B6, B12, K + Testosterone 4 Nimmervoll

5.  Higher plants, most animals by D-Glucose  liver (mammals)  kidney (birds, amphibian, reptils) 5 Nimmervoll

6.  Quickly in whole small intestine  Pass cell mebrane (oxidized, lipophilic form)  Reduction to Ascorbic acid  With circulation to organs  Absorbing capacity: 1000mg 6 Nimmervoll

7.  Biochemical functions:  Hydrogen donator (electrons)  Hydrogen acceptor (electrons)  Hold up body-functions:  Sore cure and keloid  Sterotoninbiosynthesis  Synthesis of THF and folic acid  Resistance infectious disease  Ferric resorption  Radical catcher 7 Nimmervoll

8.  Materials:  Ascorbic acid  2.6.-Dichlorphenolindophenol  Oxalic acid  Chloroform  testing tubes 8 Nimmervoll 2.6.-Dichlorphenolindophenol Leuko - 2.6.-Dichlorphenolindophenol

9.  Implementation  Make 2.6.-Dichlorphenolindophenol (  =0,4 g/l)  Make ascorbic acid standard (  =2,5 g/l)  2ml water in testing tube, and 0,5/1/1,5/2/2,5ml ascorbic acid  + small quantity oxalic acid  + 0,1ml 2.6.-DCPIP  Shake it  Wait 15 sec.  + 1,5ml chloroform  add step-by-step 2.6.-DCPIP till load transfer point 9 Nimmervoll

10. 2.6.-DCPIP blanksample 10 Nimmervoll

11.  http://www.lebensmittelwissen.de/chemie/ ascorbinsaeure/eigenchaften-vitamin-c.php Nimmervoll 11 The EU-Comenius Life-long-learning-program is financially granted by the European Commission.