2. Genetics and Heredity

3. Genetics The study of heredity, how traits are passed from parent to offspring x = or

4. The study of heredity started with the work of Gregor Mendel and his pea plant garden Mendel was an Austrian Monk that lived in the mid 1800’s

5. Inheritance Theory Prior to Mendel 1. Traits “blended” Trait: characteristics to be passed from parent to offspring “bloodlines”: thought traits passed through the blood 2. Problem with blending: cannot account for unexpected traits

6. Mendel noted that the size of pea plants varied. He cross-bred these pea plants to find some surprising results.

7. Steps of Mendel's Experiment

8. Mendel’s cross between tall pea plants yielded all tall pea plants. His cross between small pea plants yielded all small pea plants. Mendels’ cross between tall pea plants and small pea plants yielded all tall pea plants. X = x = X =

9. Here we crossed two peas which contained both tall and short information. T T t t Tt TT tt

10. When Mendel crossed these second generation tall pea plants he ended up with 1 out 4 being small. x =

11. A cross in which only one trait is studied is called monohybrid cross.monohybrid cross

12. 5. Mendel named every generation: Starting generation – P (parent) generation. The following offspring generation was called F1 - first generation(daughter generation), F2 - second filial generation, and so on.

13. PF1F2

14. Mendelian Genetics Mendel studied a number of characteristics in pea plants including: Height - short or TALL

15. Mendelian Genetics Mendel studied a number of characteristics in pea plants including: Height - short or TALL Seed color - green or YELLOW

16. Mendelian Genetics Mendel studied a number of characteristics in pea plants including: Height - short or TALL Seed color - green or YELLOW Seed shape - wrinkled or ROUND

17. Mendelian Genetics Mendel studied a number of characteristics in pea plants including: Height - short or TALL Seed color - green or YELLOW Seed shape - wrinkled or ROUND Seed coat color - white or GRAY

18. Mendelian Genetics Mendel studied a number of characteristics in pea plants including: Height - short or TALL Seed color - green or YELLOW Seed shape - wrinkled or ROUND Seed coat color - white or GRAY Pod shape - constricted or SMOOTH

19. Mendelian Genetics Mendel studied a number of characteristics in pea plants including: Height - short or TALL Seed color - green or YELLOW Seed shape - wrinkled or ROUND Seed coat color - white or GRAY Pod shape - constricted or SMOOTH Pod color - yellow or GREEN

20. Mendelian Genetics Mendel studied a number of characteristics in pea plants including: Height - short or TALL Seed color - green or YELLOW Seed shape - wrinkled or ROUND Seed coat color - white or GRAY Pod shape - constricted or SMOOTH Pod color - yellow or GREEN Flower position - terminal or AXIAL

21. Mendelian Genetics We will work with the following three: Height - short or TALL Seed color - green or YELLOW Seed shape - wrinkled or ROUND Seed coat color - white or GRAY Pod shape - constricted or SMOOTH Pod color - yellow or GREEN Flower position - terminal or AXIAL

22. Mendel’s work led him to the understanding that traits such as plant height are carried in pairs of information not by single sets of information. -Carrying the information are chromosomes. -Chromosomes are made up of sections called genes. -Genes are made up of DNA

24. DNA D.N.A. - Deoxyribonucleic Acid Molecule made of: 1. Deoxy Sugar 2. Combination of four nitrogen bases Either:a. Guanine b. Cytocine c. Thymine d. Adenine The sum total of combinations that these four bases are capable of creating are greater than all the stars visible in the night time sky

25. DNA Nitrogen bases pair up –Cytosine & Guanine –Thymine & Adenine Pairing creates a ladder shape Angle of bonds creates a twist Ladder and Twist produces the famous “Double Helix”

26. DNA DNA resides in all cells –Inside the nucleus Each strand forms a chromosome Cell Nucleus DNA

27. DNA is found in all living cells –It controls all functions inside a cell –It stores all the genetic information for an entire living organism –Single cell like an amoeba –Multi cell like a human

28. Genetics Small sections of DNA are responsible for a “trait”. These small sections are called “Genes”. –Gene - A segment of DNA that codes for a specific trait –Trait - A characteristic an organism can pass on to it’s offspring through DNA Gene

29. Phenotype –Physical characteristics

30. Genotype Phenotype –Physical characteristics Genotype –Genes we inherit from our parents

31. Phenotype –Facial structure Notice the similarities:

32. Phenotype –Facial structure –Eyes Notice the similarities:

33. Phenotype –Facial structure –Eyes –Smile Notice the similarities:

34. Phenotype –Facial structure –Eyes –Smile –Ears Notice the similarities:

35. Phenotype –Facial structure –Eyes –Smile –Ears –Nose Notice the similarities:

36. Phenotype –Facial structure –Eyes –Smile –Ears –Nose –Neck Notice the similarities:

37. Genetics There are three basic kinds of genes: –Dominant - A gene that is always expressed and hides others –Recessive - A gene that is only expressed when a dominant gene isn’t present –Codominant - Genes that work together to produce a third trait

38. Predicting Inheritance To determine the chances of inheriting a given trait, scientists use Punnett squares and symbols to represent the genes. UPPERCASE letters are used to represent dominant genes. lowercase letters are used to represent recessive genes.

39. Predicting Inheritance For example: T = represents the gene for TALL in pea plants t = represents the gene for short in pea plants So: TT & Tt both result in a TALL plant, because T is dominant over t. t is recessive. tt will result in a short plant. Remember there are two genes for every trait! One from each parent.

40. Predicting Inheritance For example: T = represents the gene for TALL in pea plants t = represents the gene for short in pea plants So: TT & Tt both result in a TALL plant, because T is dominant over t. t is recessive. tt will result in a short plant. Remember there are two genes for every trait! Mendels’ Principle of Dominance Some genes (alleles) are dominant and others are recessive. The phenotype (trait) of a dominant gene will be seen when it is paired with a recessive gene.

41. Predicting Inheritance Let’s cross a totally dominant tall plant (TT) with a short plant (tt). Each plant will give only one of its’ two genes to the offspring or F 1 generation. TT x tt TTtt

42. Predicting Inheritance Let’s cross a totally dominant tall plant (TT) with a short plant (tt). Each plant will give only one of its’ two genes to the offspring or F 1 generation. TT x tt TTtt Mendels’ “Law” of Segregation Each gene (allele) separates from the other so that the offspring get only one gene from each parent for a given trait.

43. Punnett Squares TtTtTt The genes from one parent go here. The genes from the other parent go here.

44. Punnett Squares TT tTtTtTt t

45. Punnett Squares TT tTtTtTt tTtTt

46. Punnett Squares TT tTtTtTtTt tTtTtTtTt

47. TT tTt tTtTtTtTt

48. Punnett Squares TT tTt t

49. Punnett Squares TT tTt t F 1 generation

50. Interpreting the Results The genotype for all the offspring is Tt. The genotype ratio is: Tt - 4/4 The phenotype for all the offspring is tall. The phenotype ratio is: tall - 4/4

51. So Let’s Apply What We Know

52. Genetics Dominant and Recessive Genes A dominant gene will always mask a recessive gene. A “widows peak” is dominant, not having a widows peak is recessive. If one parent contributes a gene for a widows peak, and the other parent doesn’t, the off- spring will have a widows peak. Widows Peak

53. Genetics We can use a “Punnet Square” to determine what pairs of genes Lilly has Wwww Wwww w w Ww Assume Lilly is heterozygous Ww Assume Herman is homoozygous recessive ww A Punnet Square begins with a box 2 x 2 One gene is called an “allele” One parents pair is split into alleles on top, the other along the side Each allele is crossed with the other allele to predict the traits of the offspring

54. Genetics Notice that when Lilly is crossed with Herman, we would predict that half the offspring would be “Ww”, the other half would be “ww” Half “Ww”, Heterozygous, and will have a widows peak Half “ww”, Homozygous, and will not have a widows peak Wwww Wwww w w Ww

55. Genetics Punnet Square - A tool we use for predicting the traits of an offspring –Letters are used as symbols to designate genes –Capital letters are used for dominant genes –Lower case letters are used for recessive genes –Genes always exist in pairs

56. Genetics A Widows Peak, dominant, would be symbolized with a capital “W”, while no widows peak, recessive, would be symbolized with a lower case “w”. Father - No Widows Peak - w Mother - Has a Widows Peak - W

57. Genetics All organisms have two copies of each gene, one contributed by the father, the other contributed by the mother. Homozygous - Two copies of the same gene Heterozygous - Two different genes

58. Genetics For the widows peak: WW - has a widows peak Homozygous dominant Ww - has a widows peak Heterozygous ww - no widows peak Homozygous recessive

59. Genetics Since Herman has no widows peak, he must be “ww”, since Lilly has a widows peak she could be either “WW” or “Ww” Definitely ww Homozygous recessive Either Ww Heterozygous or WW Homozygous dominant

60. Genetics Another possibility is that Lilly might be “WW”, homozygous dominant. Ww w w WW Assume Lilly is homozygous dominant WW Assume Herman is homoozygous ww Ww Notice that all the offspring are heterozygous and will have a widows peak

61. Genetics So which is true? Is Lilly homozygous dominant (WW) or is she heterozygous (Ww)? Ww w w WW ww Wwww w w Ww

62. Genetics Ww w w WW ww Wwww w w Ww If Lilly were heterozygous, then 1 / 2 of their offspring should have a widows peak, 1 / 2 shouldn’t If Lilly were homozygous, all of their children will have a widows peak

63. Genetics Recall that Herman and Lilly had another offspring, Marylin. She had no widows peak, therefore, Lilly must be heterozygous.

64. Genetics So, back to the original question. What color hair will the offspring of Prince Charming and Snow White have?

65. Genetics Hair color is different from widows peak, no color is truly dominant. –Brown and blond are the two, true traits –Homozygous conditions produce either brown or blond hair –Heterozygous conditions produce red hair

66. Genetics For Snow White to have brown hair she must be homozygous dominant, “BB”, a blond Prince Charmin must be homozygous recessive, “bb”. Bb b b BB

67. Genetics Hair color is a perfect example of a trait Prince Charming is blond Snow White has dark hair What color hair should their children have?

68. Genetics All the offspring from Prince Charming and Snow White will therefore be heterozygous, “Bb”, and since hair color is codominant….. all their children will have red hair. +

69. http://www.bozemanscience.com /beginners-guide-to-punnett- squares

70. TEST CROSS Cross between an organism with an unknown genotype and an organism with the recessive phenotype. http://www.youtube.com/watch?v=IK5QW 1UfAuI

71. Complete page test cross on page 185

72. Cell Division (Meiosis) 1. A process of cell division where the number of chromasomes is cut in half 2. Occurs in gonads (testes, ovaries, stamens, etc) 3. Makes gametes (sperm, ova, pollen, etc)