1. Cell Biology I. Overview II. Membranes: How Matter Get in and Out of Cells III. Cellular Respiration IV. Photosynthesis V. DNA, RNA, and Chromosome Structure VI. Protein Synthesis VII. Cellular Reproduction

2. VII. CELL REPRODUCTION Overview: Why Reproduce?

3. VII. CELL REPRODUCTION Overview: Why Reproduce? X X X X Reproduction is selected for, by definition.

4. - WHY?? Bigger is better…. As cells increase in size, they decrease in efficiency.

5. - WHY?? Bigger is better…. So selection favors growth… But as cells increase in size, they decrease in efficiency.

6. The “surface area to volume ratio” decreases as something increases in size…. SA/V = 6SA/V = 3SA/V = 1.5

7. The “surface area to volume ratio” decreases as something increases in size…. The surface area – the membrane – limits the rate of supply of nutrients to the cell. The volume – where all the enzymes are – represents potential production and ‘demand’ for nutrients. SA/V = 6SA/V = 3SA/V = 1.5

8. So, as something gets larger, the volume increases more than the surface area… and the demand for nutrients (to meet peak productivity) grows faster than the rate at which the more slowly increasing SA can supply them. So, supply fails to meet demand, and the cell cannot meet peak productivity… it becomes less efficient. SA/V = 6SA/V = 3SA/V = 1.5

9. - WHY?? Bigger is better…. So selection favors growth… But as cells increase in size, they decrease in efficiency. So, to get bigger, most organisms increase cell number, not cell size….

10. - WHY?? And with many cells, cell specialization can increase efficiency at the organismal level.

11. LE 12-5 G1G1 G2G2 S (DNA synthesis) INTERPHASE Cytokinesis MITOTIC (M) PHASE Mitosis VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle

12. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle 1. Interphase: a. G1: high metabolic activity (protein synthesis) chromosomes diffuse; one DNA double helix per chromosome

13. VIII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle 1. Interphase: Some cell types are "stuck" in this stage when they mature... it is only "stem cells" that keep dividing. In some tissues, all stem cells eventually mature, so the tissue can't regenerate (neurons)

14. LE 12-15 G1G1 G 1 checkpoint G1G1 G0G0 If a cell receives a go-ahead signal at the G 1 checkpoint, the cell continues on in the cell cycle. If a cell does not receive a go-ahead signal at the G 1 checkpoint, the cell exits the cell cycle and goes into G 0, a nondividing state.

17. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle 1. Interphase a. G1 b. S Chromosome duplication (including DNA synthesis) Centromere Sister chromatids

18. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle 1. Interphase a. G1 b. S c. G2

19. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle 1. Interphase 2. Mitosis

20. VIII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1. Initiation - Replisomes land at "origin" - They have a variety of enzymes that work together, including: HELICASE that unzips the double helix. PRIMASE, an RNA POLYMERASE, that starts replication DNA POLYMERASES that make DNA.

21. 5' 3' - HELICASE separates strands

22. - PRIMASE lays down RNA from 5' 3' (like transcription) 5' 3'

23. 5' 3' - HELICASE separates strands - PRIMASE lays down RNA from 5' 3' (like transcription) - DNA POLYMERASE - 'knocks' primase off and adds DNA bases to the free 3' OH group....

24. 5' 3' So, into each 'replication fork',there is "continuous synthesis" on one strand "FORK" VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork

25. 5' 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork What about the other strands?

26. 5' 3' VIII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork What about the other strands? PROBLEM!!! Only 5' phosphates available.....so they can't be extended 5'

27. 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork The process must start again.... 5' Primase makes RNA 3'

28. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork The process must start again.... 5' 3' 5' DNA POLY adds DNA

29. 5' 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork And what happens when more DNA is opened up???? 5' 3' 5'

30. 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork Continuous synthesis can continue from 3' end of DNA 5' 3' 5'

31. 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork But Primase must begin the process on "lagging" strand 5' 3' 5'

32. 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork...then DNA POLY can add DNA bases to "primer" 5' 3' 5'

33. 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork And this happens at the other fork...... DNA is opened... 5' 3' 5' 3'

34. 5' 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork Continuous synthesis occurs from the free 3' end 5' 3' 5' 3'

35. 5' 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork Discontinuous synthesis on "lagging strand" (Primase, etc) 5' 3' 5' 3' 5'

36. 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork So, BOTH STRAND HAVE "OKAZAKI FRAGMENTS...." 5' 3' 5' 3' 5'

37. 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork NOW WHAT??? We have RNA in the DNA double helix 5' 3' 5' 3' 5'

38. 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork Repair DNA Polymerases cut RNA out and add DNA to 3' 3'

39. 5' 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork 3.DNA Repair Repair DNA Polymerases cut RNA out and add DNA to 3' 3'

40. 5' 3' VIII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork 3.DNA Repair Final linkage between DNA frag's made by LIGASE 3' 5'3' 5' 3' H2OH2O

41. 5' 3' VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork 3.DNA Repair Final linkage between DNA frag's made by LIGASE 3' 5'3' 5' H2OH2O REPLICATION COMPLETE

42. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication 1.Initiation 2.Replication at the fork 3.DNA Repair Semi-conservative replication

43. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication C. Mitosis

44. INTERPHASEPROPHASEPROMETAPHASE LE 12-6aa Centrosomes (with centriole pairs Chromatin (duplicated) Early mitotic spindle Nucleus Nuclear envelope Plasma membrane Aster Centromere Chromosome, consisting of two sister chromatids Fragments of nuclear envelope Kinetochore Nonkinetochore microtubules Kinetochore microtubule

45. LE 12-6ba Centrosome at one spindle pole Metaphase plate Spindle Cleavage furrow Daughter chromosomes Nucleolus forming Nuclear envelope forming METAPHASEANAPHASETELOPHASE

46. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication C. Mitosis G 2 OF INTERPHASEPROPHASEPROMETAPHASE

47. LE 12-6da METAPHASEANAPHASE TELOPHASE AND CYTOKINESIS VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication C. Mitosis

48. LE 12-10 Nucleus Cell plate Chromosomes Nucleolus Chromatin condensing 10 µm Prophase. The chromatin is condensing. The nucleolus is beginning to disappear. Although not yet visible in the micrograph, the mitotic spindle is starting to form. Prometaphase. We now see discrete chromosomes; each consists of two identical sister chromatids. Later in prometaphase, the nuclear envelope will fragment. Metaphase. The spindle is complete, and the chromosomes, attached to microtubules at their kinetochores, are all at the metaphase plate. Anaphase. The chromatids of each chromosome have separated, and the daughter chromosomes are moving to the ends of the cell as their kinetochore micro- tubules shorten. Telophase. Daughter nuclei are forming. Meanwhile, cytokinesis has started: The cell plate, which will divide the cytoplasm in two, is growing toward the perimeter of the parent cell. VII. CELL REPRODUCTION Overview: Why Reproduce? A. The Cell Cycle B. DNA Replication C. Mitosis

49. LE 12-9a Cleavage furrow 100 µm Contractile ring of microfilaments Daughter cells Cleavage of an animal cell (SEM)

50. LE 12-9b 1 µm Daughter cells Cell plate formation in a plant cell (TEM) New cell wall Cell plate Wall of parent cell Vesicles forming cell plate