1. Molecular beacon fluorescence detection of pseudogene SHMT-psI using two molecular beacon probes, E8/E10 and E9/E10

2. Evanescent Wave Sensing

3. Cladding Core Exciting Laser Fluorescence Generated at Surface Fluorescence coupled into fiber. Fluorophore labeled Phospho-STAT3 antibodies. Phospho-STAT3 Phospho-STAT3 antibodies (IgG) Detection of Phosphorylated STAT3 Protein by Fiber-Based Sensor STAT - Signal Transducer and Activator of Transcription protein

4. Kretschmann (ATR) geometry used to excite surface plasmons Surface Plasmon Sensors

5. Surface plasmon resonance curves. The left-hand side curve is for just the silver film (labeled Ag); the right-hand side shows the curve (labeled Ag/p-4-BCMU) shifted on the deposition of a monolayer Langmuir-Blodgett film of poly-4-BCMU on the silver film

6. Close packing of colloidal nanospheres to form a photonic crystal of close-packed colloidal array. (Left) Atomic Force Microscope (AFM) image of the surface layer. (Right) Scanning Electron Microscope (SEM) image of a cross-section (Markowicz and Prasad; unpublished). Photonic Crystal Sensors

7. Visible extinction spectra showing how diffraction depends on the glucose concentration for a 125µm- thick PCCA glucose sensor. The ordinate is given as –log T, where T is the transmittance. The PCCA expands for concentrations between 0.1 and 0.5 mM glucose.

9. Schematics of a five parameter flow cytometer showing details of collection scheme.

10. Various parameters monitored

12. Applications HIV Monitoring Leukemia or Lymphoma Immunophenotyping Organ Transplant Monitoring DNA Analysis for Tumor Ploidy and SPF Primary and Secondary Immunodeficiency Bacterial contamination in food and water

13. Biophotonics Opportunities Modeling of light propagation in tissue In-vivo Bioimaging, Spectroscopy, and Optical Biopsy Real-Time monitoring of Drug Interactions Optical Biopsy Nano-Biophotonic Probes Multiphoton Processes for Biotechnology Femtolaser based biotechnology\ Nanoarray multianalytic sensors Multiparameter Molecular Flowcytometry Single Molecule Biofunctions Nanomedicine

14. Acknowledgements Researchers at the Institute:  Prof. E. Bergey  Prof. A. Cartwright  Prof. M. Swihart  Prof. E. Furlani  Dr. A. Kachynski  Dr. A. Kuzmin  Dr. Y. Sahoo  Dr. H. Pudavar  Dr. T. Ohulchanskyy  Dr. D. Bharali  Dr. D. Lucey  Dr. K. Baba  Dr. J. Liu DURINT/AFSOR  Dr. Charles Lee Outside Collaborators  Prof. R.Boyd  Prof. J.Haus  Prof. J M J Frechet  Prof. M. Stachowiak  Dr. A. Oseroff  Dr. R. Pandey  Dr. J. Morgan  Dr. P Dandona

15. “Lighting the Way to Technology through Innovation” The Institute for Lasers, Photonics and Biophotonics University at Buffalo Biophotonics P.N.Prasad www.biophotonics.buffalo.edu